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| Registros recuperados : 199 | |
| 84. |  | CATARINO, A. M.; FAJARDO, T. V. M.; PIO-RIBEIRO, G.; NICKEL, O.; REVERS, L. F. Absolute quantification of grapevine leafroll-associated virus 4 by Taqman real time RT-PCR in infected grapevines. Journal of the Brazilian Society for Virology, Novo Hamburgo, v. 18, p. 184-185, 2013. Suplemento. Resumo publicado nos Annals of XXIV Brazilian Congress of Virology & VIII Mercosur Meeting of Virology September, 01-04, 2013, Porto Seguro, Bahia.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 85. | | DE VILLA, F. Z.; ECKERT, C.; NICKEL, O.; FAJARDO, T. V. M. Avaliação de longo prazo do efeito de eliminação de Apple stem grooving virus por quimioterapia em macieira cv. Royal Gala. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA UVA E VINHO, 12., ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 8., 2014, Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2014. p. 12| Biblioteca(s): Embrapa Uva e Vinho. |
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| 88. | | FAJARDO, T. V. M.; NASCIMENTO, M. B.; EIRAS, M.; NICKEL, O.; PIO-RIBEIRO, G. Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil. Ciência Rural, Santa Maria, v. 45, n. 12, p. 2197-2200, dez. 2015.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 89. | | MOURA, C. J. M. de; FAJARDO, T. V. M.; EIRAS, M.; NICKEL, O. Levantamento e caracterização molecular de vírus e viroide em vinhedos no Município de São Roque, SP. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 14. ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 10., 2016, Bento Gonçalves. Resumos...Bento Gonçalves, RS: Embrapa uva e Vinho, 2016. p. 26. (Embrapa uva e Vinho. Documentos, 99)| Biblioteca(s): Embrapa Uva e Vinho. |
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| 92. | | RADAELLI, P.; FAJARDO, T. V. M.; NICKEL, O.; EIRAS, M.; PIO-RIBEIRO, G. Coat protein gene expression of Grapevine virus B in Escherichia coli and polyclonal antibody production. Tropical Plant Pathology, Brasília, DF, v. 33, p. S286, 2008. Suplemento. Resumo.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 96. | | RADAELLI, P.; FAJARDO, T. V. M.; EIRAS, M.; KUHN, G. B.; PIO-RIBEIRO, G.; NICKEL, O. Caracterização molecular parcial do gene da proteína capsidial do Grapevine fanleaf virus. Fitopatologia Brasileira, Brasília, DF, v. 32, p. S 129 ago. 2007. Suplemento. Edição dos resumos do XL Congresso Brasileiro de Fitopatologia, Maringá, 13 a 17 de agosto de 2007. Resumo 97.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 97. | | NASCIMENTO, M. B.; FAJARDO, T. V. M.; EIRAS, M.; NICKEL, O.; PIO-RIBEIRO, G. Caracterização molecular parcial de um isolado de Prunus necrotic ringspot virus de roseira. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA UVA E VINHO, 12., ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 8., 2014, Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2014. p. 11.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 98. | | MEISSNER FILHO, P. E.; SOUZA JÚNIOR, M. T.; DANTAS, J. L. L.; NICKEL, O.; GONSALVES, D. Avaliação da resistência de mamoeiros transgênicos a um isolado do Papaya risgspot virus. Fitopatologia Brasileira, Brasília, DF, v. 29, supl., p. S145, ago. 2004. Edição dos Resumos do XXXVII Congresso Brasileiro de Fitopatologia, Gramado, RS, ago. 2004.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 99. | | NICKEL, O.; FAJARDO, T. V. M.; BERNARDI, J.; SINSKI, I.; SILVA, F. N. DA; BOGO, A. Ocorrência de vírus em morangos no Rio Grande do Sul e detecção biológica e molecular. In: SIMPÓSIO NACIONAL DO MORANGO, 3.; ENCONTRO SOBRE PEQUENAS FRUTAS E FRUTAS NATIVAS DO MERCOSUL, 2., 2006, Pelotas. Resumos... Pelotas: Embrapa Clima Temperado, 2006. (Embrapa Clima Temperado. Documentos, 167). 1 CD-ROM. Resumo.| Biblioteca(s): Embrapa Uva e Vinho. |
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| Registros recuperados : 199 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
23/10/2017 |
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Data da última atualização: |
06/05/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
FAJARDO, T. V. M.; SILVA, F. N.; EIRAS, M.; NICKEL, O. |
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Afiliação: |
THOR VINICIUS MARTINS FAJARDO, CNPUV; Fábio N. Silva, Universidade do Estado de Santa Catarina, Lages, SC 88520-000, Brazil; Marcelo Eiras, Instituto Biológico de São Paulo, São Paulo, SP 04014-002, Brazil; OSMAR NICKEL, CNPUV. |
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Título: |
High-throughput sequencing applied for the identification of viruses infecting grapevines in Brazil and genetic variability analysis. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Tropical Plant Pathology, v. 52, p. 250-260, 2017. |
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DOI: |
10.1007/s40858-017-0142-8 |
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Idioma: |
Português |
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Conteúdo: |
The application of high-throughput sequencing technologies (HTS) enables the recovery of many nucleotide sequence fragments from diseased plants and may help in pathogen identification. This study was designed to identify viruses infecting 15 grapevine (Vitis spp.) samples collected from experimental fields and vine collections and assess the genetic variability of the identified viruses. The virus-enriched dsRNAs were extracted from bark scrapings and sequenced using an Illumina platform. The paired-end reads were analyzed, assembled contigs were generated and identified as related to viruses. Contigs of 14 viruses have been identified, some of them covering large extensions of viral genomes or resulting in assembly of near-complete or complete genomes. Grapevine virus infections are usually mixed and the HTS assays were suitable to identify ten viruses already reported that traditionally infect grapevines in Brazil, one that has been recently identified (Grapevine Syrah virus 1) and others (Grapevine Cabernet Sauvignon reovirus, Grapevine Red Globe virus and Grapevine vein clearing virus) not previously reported in this country. Nucleotide identities among Brazilian isolates identified by HTS and homologous grapevine virus sequences in GenBank were high, ranging from 77% to 99%. Genetic variability analysis of viral sequences obtained by HTS and sequences available in GenBank indicated that the coding regions in the different viral species are under purifying selection, and that recombination events occurred in the majority of the viral species analyzed. The coat protein genes, generally, had lower genetic variability than the replicase and movement protein genes. Keywords Vitis . Diagnosis . HTS . Next-generation sequencing . NGS . Variability MenosThe application of high-throughput sequencing technologies (HTS) enables the recovery of many nucleotide sequence fragments from diseased plants and may help in pathogen identification. This study was designed to identify viruses infecting 15 grapevine (Vitis spp.) samples collected from experimental fields and vine collections and assess the genetic variability of the identified viruses. The virus-enriched dsRNAs were extracted from bark scrapings and sequenced using an Illumina platform. The paired-end reads were analyzed, assembled contigs were generated and identified as related to viruses. Contigs of 14 viruses have been identified, some of them covering large extensions of viral genomes or resulting in assembly of near-complete or complete genomes. Grapevine virus infections are usually mixed and the HTS assays were suitable to identify ten viruses already reported that traditionally infect grapevines in Brazil, one that has been recently identified (Grapevine Syrah virus 1) and others (Grapevine Cabernet Sauvignon reovirus, Grapevine Red Globe virus and Grapevine vein clearing virus) not previously reported in this country. Nucleotide identities among Brazilian isolates identified by HTS and homologous grapevine virus sequences in GenBank were high, ranging from 77% to 99%. Genetic variability analysis of viral sequences obtained by HTS and sequences available in GenBank indicated that the coding regions in the different viral species are under purifying selection, an... Mostrar Tudo |
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Palavras-Chave: |
Diagnosis; HTS; Next-generation sequencing; NGS. |
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Thesaurus NAL: |
variability; Vitis. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/197010/1/Fajardo2017-Article-High-throughputSequencingAppli.pdf
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Marc: |
LEADER 02498naa a2200241 a 4500
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005 2019-05-06
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1007/s40858-017-0142-8$2DOI
100 1 $aFAJARDO, T. V. M.
245 $aHigh-throughput sequencing applied for the identification of viruses infecting grapevines in Brazil and genetic variability analysis.$h[electronic resource]
260 $c2017
520 $aThe application of high-throughput sequencing technologies (HTS) enables the recovery of many nucleotide sequence fragments from diseased plants and may help in pathogen identification. This study was designed to identify viruses infecting 15 grapevine (Vitis spp.) samples collected from experimental fields and vine collections and assess the genetic variability of the identified viruses. The virus-enriched dsRNAs were extracted from bark scrapings and sequenced using an Illumina platform. The paired-end reads were analyzed, assembled contigs were generated and identified as related to viruses. Contigs of 14 viruses have been identified, some of them covering large extensions of viral genomes or resulting in assembly of near-complete or complete genomes. Grapevine virus infections are usually mixed and the HTS assays were suitable to identify ten viruses already reported that traditionally infect grapevines in Brazil, one that has been recently identified (Grapevine Syrah virus 1) and others (Grapevine Cabernet Sauvignon reovirus, Grapevine Red Globe virus and Grapevine vein clearing virus) not previously reported in this country. Nucleotide identities among Brazilian isolates identified by HTS and homologous grapevine virus sequences in GenBank were high, ranging from 77% to 99%. Genetic variability analysis of viral sequences obtained by HTS and sequences available in GenBank indicated that the coding regions in the different viral species are under purifying selection, and that recombination events occurred in the majority of the viral species analyzed. The coat protein genes, generally, had lower genetic variability than the replicase and movement protein genes. Keywords Vitis . Diagnosis . HTS . Next-generation sequencing . NGS . Variability
650 $avariability
650 $aVitis
653 $aDiagnosis
653 $aHTS
653 $aNext-generation sequencing
653 $aNGS
700 1 $aSILVA, F. N.
700 1 $aEIRAS, M.
700 1 $aNICKEL, O.
773 $tTropical Plant Pathology$gv. 52, p. 250-260, 2017.
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Embrapa Uva e Vinho (CNPUV) |
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