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| Registros recuperados : 173 | |
| 9. |  | NICIURA, S. C. M.; THOLON, P. Absence of CpG observed/expected ratio depletion in Haemonchus contortus evidences a non-methylated genome. In: WORKSHOP ON OMICS STRATEGIES APPLIED TO LIVESTOCK SCIENCE, 1., 2017, Piracicaba, SP. Proceedings... São Carlos, SP: Embrapa Pecuária Sudeste, 2017. p. 21. (Embrapa Pecuária Sudeste. Documentos, 125) Editores: Luiz Lehmann Coutinho, ESALQ/USP; Luciana Correia de Almeida Regitano, Embrapa Pecuária Sudeste; Gerson Barreto Mourão, ESALQ/USP; Aline Silva Mello Cesar, ESALQ/USP; Bárbara Silva Vignato, FZEA/USP; Mirele Daiana Poleti,...| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 11. | | CRUVINEL, G. G.; MORAES, C. V.; NICIURA, S. C. M. Genotipagem de marcadores moleculares de resistência ao parasita Haemonchus contortus em ovinos da raça Morada Nova. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 9., 2017, São Carlos, SP. Anais... São Carlos, SP: Embrapa Pecuária Sudeste; Embrapa Instrumentação, 2017. p. 43. (Embrapa Pecuária Sudeste. Documentos, 126). Editores técnicos: Alexandre Berndt, Ana Rita Araujo Nogueira, Bianca Baccili Zanotto Vigna, Juliana Gonçalves Costa, Lea Chapaval, Manuel Antonio Chagas Jacinto, Patricia Menezes Santos.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 12. | | MORAES, C. V.; CRUVINEL, G. G.; NICIURA, S. C. M. Investigação da ocorrência de eventos epigenéticos em Haemonchus contortus em estudo da resistência ao monepantel. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 9., 2017, São Carlos, SP. Anais... São Carlos, SP: Embrapa Pecuária Sudeste; Embrapa Instrumentação, 2017. p. 18. (Embrapa Pecuária Sudeste. Documentos, 126). Editores técnicos: Alexandre Berndt, Ana Rita Araujo Nogueira, Bianca Baccili Zanotto Vigna, Juliana Gonçalves Costa, Lea Chapaval, Manuel Antonio Chagas Jacinto, Patricia Menezes Santos.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 15. | | NICIURA, S. C. M.; PERECIN, F.; SARAIVA, N. Z. Controle epigenético do desenvolvimento e do envelhecimento em mamíferos. In: NICIURA, S. C. M.; SARAIVA, N. Z. (Ed.). Epigenética: bases moleculares, efeitos na fisiologia e na patologia, e implicações para a produção animal e a vegetal. Brasília, DF: Embrapa, 2014. Cap. 7, p. 155-187.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 17. | | CRUVINEL, G. G.; MORAES, C. V.; NICIURA, S. C. M. Variabilidade genética para marcadores SNP associados à resistência a Haemonchus contortus em ovinos Morada Nova. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 10., 2018, São Carlos, SP. Anais... São Carlos, SP: Embrapa Instrumentação; Embrapa Pecuária Sudeste, 2018. p. 19. (Embrapa Instrumentação. Documentos, 68). Editores técnicos: Daniel Souza Corrêa, Elaine Cristina Paris, Maria Alice Martins, Paulino Ribeiro Villas Boas, Wilson Tadeu Lopes da Silva.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 20. | | MARGATHO, L. F. F.; NICIURA, S. C. M.; VERISSIMO, C. J.; MOLENTO, M. B. Situação da resistência à anti-helmínticos em um rebanho no município de Piratinga, SP, Brasil. O Biológico, av. 71, n. 2, p. 119, 2009| Biblioteca(s): Embrapa Pecuária Sudeste. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste; Embrapa Pecuária Sul. |
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Data corrente: |
16/11/2022 |
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Data da última atualização: |
16/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
NICIURA, S. C. M.; OKINO, C. H.; NUCCI, A. DA S.; MALAGO JUNIOR, W.; BENAVIDES, M. V.; ESTEVES, S. N.; CHAGAS, A. C. de S. |
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Afiliação: |
SIMONE CRISTINA MEO NICIURA, CPPSE; CINTIA HIROMI OKINO, CPPSE; ALESSANDRA DA SILVA NUCCI, Centro Universitário Central Paulista; WILSON MALAGO JUNIOR, CPPSE; MAGDA VIEIRA BENAVIDES, CPPSUL; SERGIO NOVITA ESTEVES, CPPSE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
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Título: |
Polymorphisms in exon 11 of the mptl-1 gene and monepantel resistance in Haemonchus contortus. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Parasitology Research, v. 121, p. 3581-3588, 2022. |
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DOI: |
https://doi.org/10.1007/s00436-022-07682-6 |
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Idioma: |
Inglês |
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Conteúdo: |
Chemical treatments are the main strategy to control gastrointestinal nematodes in sheep, and the emergence of anthelmintic resistance, as consequence, results in control failures and leads to economic losses. Thus, molecular tests may constitute an excellent tool for the early detection of anthelmintic resistance-related mutations. Thus, a polymerase chain reaction (PCR)-based genotyping assay followed by polyacrylamide gel electrophoresis (PAGE) was developed to detect polymorphisms in exon 11 of the acetylcholine receptor monepantel-1 gene (mptl-1) that were previously associated with monepantel resistance through a genome-wide study in Haemonchus contortus. DNA samples recovered from individual and pooled third-stage larvae from two susceptible feld-derived isolates and fve (three in vivo-derived and two feld-derived) resistant populations were used. New polymorphisms, including a 6-bp deletion and a 3-bp insertion, were detected in resistant individuals. These indels, confrmed using sequencing of cloned PCR products, are predicted to result in amino acid changes in transmembrane domain 2 (TMD2) of the MPTL-1 protein. The two susceptible isolates showed only the presence of the wild-type allele (100%), whereas lower frequencies of the wild-type allele were detected in monepantel-resistant populations (11.1 to 66.7%). These fndings report new polymorphisms in the mptl-1 gene, validate the results obtained through genomic mapping for monepantel resistance, and provide a PCR-based assay to genotype indels located in exon 11 of mptl-1 in H. contortus. MenosChemical treatments are the main strategy to control gastrointestinal nematodes in sheep, and the emergence of anthelmintic resistance, as consequence, results in control failures and leads to economic losses. Thus, molecular tests may constitute an excellent tool for the early detection of anthelmintic resistance-related mutations. Thus, a polymerase chain reaction (PCR)-based genotyping assay followed by polyacrylamide gel electrophoresis (PAGE) was developed to detect polymorphisms in exon 11 of the acetylcholine receptor monepantel-1 gene (mptl-1) that were previously associated with monepantel resistance through a genome-wide study in Haemonchus contortus. DNA samples recovered from individual and pooled third-stage larvae from two susceptible feld-derived isolates and fve (three in vivo-derived and two feld-derived) resistant populations were used. New polymorphisms, including a 6-bp deletion and a 3-bp insertion, were detected in resistant individuals. These indels, confrmed using sequencing of cloned PCR products, are predicted to result in amino acid changes in transmembrane domain 2 (TMD2) of the MPTL-1 protein. The two susceptible isolates showed only the presence of the wild-type allele (100%), whereas lower frequencies of the wild-type allele were detected in monepantel-resistant populations (11.1 to 66.7%). These fndings report new polymorphisms in the mptl-1 gene, validate the results obtained through genomic mapping for monepantel resistance, and provide a PC... Mostrar Tudo |
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Palavras-Chave: |
Amino acetonitrile derivatives; Anthelmintic resistance; PCR. |
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Thesagro: |
Haemonchus Contortus. |
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Thesaurus NAL: |
Genotyping. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02402naa a2200265 a 4500
001 2148276
005 2022-11-16
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s00436-022-07682-6$2DOI
100 1 $aNICIURA, S. C. M.
245 $aPolymorphisms in exon 11 of the mptl-1 gene and monepantel resistance in Haemonchus contortus.$h[electronic resource]
260 $c2022
520 $aChemical treatments are the main strategy to control gastrointestinal nematodes in sheep, and the emergence of anthelmintic resistance, as consequence, results in control failures and leads to economic losses. Thus, molecular tests may constitute an excellent tool for the early detection of anthelmintic resistance-related mutations. Thus, a polymerase chain reaction (PCR)-based genotyping assay followed by polyacrylamide gel electrophoresis (PAGE) was developed to detect polymorphisms in exon 11 of the acetylcholine receptor monepantel-1 gene (mptl-1) that were previously associated with monepantel resistance through a genome-wide study in Haemonchus contortus. DNA samples recovered from individual and pooled third-stage larvae from two susceptible feld-derived isolates and fve (three in vivo-derived and two feld-derived) resistant populations were used. New polymorphisms, including a 6-bp deletion and a 3-bp insertion, were detected in resistant individuals. These indels, confrmed using sequencing of cloned PCR products, are predicted to result in amino acid changes in transmembrane domain 2 (TMD2) of the MPTL-1 protein. The two susceptible isolates showed only the presence of the wild-type allele (100%), whereas lower frequencies of the wild-type allele were detected in monepantel-resistant populations (11.1 to 66.7%). These fndings report new polymorphisms in the mptl-1 gene, validate the results obtained through genomic mapping for monepantel resistance, and provide a PCR-based assay to genotype indels located in exon 11 of mptl-1 in H. contortus.
650 $aGenotyping
650 $aHaemonchus Contortus
653 $aAmino acetonitrile derivatives
653 $aAnthelmintic resistance
653 $aPCR
700 1 $aOKINO, C. H.
700 1 $aNUCCI, A. DA S.
700 1 $aMALAGO JUNIOR, W.
700 1 $aBENAVIDES, M. V.
700 1 $aESTEVES, S. N.
700 1 $aCHAGAS, A. C. de S.
773 $tParasitology Research$gv. 121, p. 3581-3588, 2022.
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