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| 2. |  | RIBEIRO, N. R.; SILVA, J. F. V.; FRANCISCO, A.; FAVORETO, L.; TOLEDO, A. M.; CAMPOS, A. S. de. Resistência de genótipos de espécies de vegetais semeados na entressafra aos nematóides Meloidogyne incognita e M. javanica. In: CONGRESSO BRASILEIRO DE NEMATOLOGIA, 24., 2003, Petrolina. [Anais...]. Petrolina: Sociedade Brasileira de Nematologia: Embrapa Semi-Árido, 2003. p. 101. Editado por Wellington Antonio Moreira, Lúcia Helena Piedade Kiill.| Biblioteca(s): Embrapa Soja. |
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| 3. | | OLIVEIRA, R. J. P. de; SILVA, S. D. dos A. e; CAMPOS, A. S. de; FONSECA, E. R.; HÄRTER, A. Período de colheita de pinhão-manso em Pelotas/RS, na safra 2013/14. In: SIMPÓSIO ESTADUAL DE AGROENERGIA; REUNIÃO TÉCNICA DE AGROENERGIA - RS, 5.; ENCONTRO DE ENERGIAS RENOVÁVEIS NA AGRICULTURA FAMILIAR, 2., Pelotas, 2014. Anais... Pelotas: Embrapa Clima Temperado, 2014. 1 pen card.| Biblioteca(s): Embrapa Clima Temperado. |
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| 6. | | CAMPOS, A. S. de; CENTENO, A.; ANDRES, A.; PARFITT, J. M. B.; MÉLLO-ARAUJO, L. B.; BUENO. M. V.; PINTO, M. A. B.; MARTINS, M. B.; VEBER, P. M.; SCIVITTARO, W. B. Utilização da tecnologia sulco-camalhão na produção de soja e milho em Terras Baixas do Rio Grande do Sul. Pelotas: Embrapa Clima Temperado, 2021. 30 p. (Embrapa Clima Temperado. Documentos, 506). ODS 2.| Biblioteca(s): Embrapa Clima Temperado. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste; Embrapa Pecuária Sul. |
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Data corrente: |
16/03/2020 |
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Data da última atualização: |
20/04/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
HAEHLING, M. B. V.; CRUVINEL, G. G.; TOSCANO, J. H. B.; GIRALDELO, L. A.; SANTOS, I. B. dos; ESTEVES, S. N.; BENAVIDES, M. V.; BARIONI JUNIOR, W.; NICIURA, S. C. M.; CHAGAS, A. C. de S. |
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Afiliação: |
MAREI BORSCH VON HAEHLING, UNESP; GIOVANNA GABRIELLE CRUVINEL, UNICEP; JOÃO HENRIQUE BARBOSA TOSCANO, UNESP; LUCIANA APARECIDA GIRALDELO, UNICEP; ISABELLA BARBOSA DOS SANTOS, UNESP; SERGIO NOVITA ESTEVES, CPPSE; MAGDA VIEIRA BENAVIDES, CPPSUL; WALDOMIRO BARIONI JUNIOR, CPPSE; SIMONE CRISTINA MEO NICIURA, CPPSE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
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Título: |
Four single nucleotide polymorphisms (SNPs) are associated with resistance and resilience to Haemonchus contortus in Brazilian Morada Nova sheep. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Veterinary Parasitology, v. 279, 2020, 109053. |
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Páginas: |
11 p. |
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ISSN: |
0304-4017 |
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DOI: |
https://doi.org/10.1016/j.vetpar.2020.109053 |
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Idioma: |
Inglês |
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Conteúdo: |
Gastrointestinal nematodes are a major constraint in sheep production. Breeding for resistance has proven to be an effective and feasible approach to address this problem. The use and investigation of genetic markers for resistance traits could accelerate genetic progress and lead to a better understanding of underlying molecular mechanisms. Thus, the aim of this study was to evaluate if five single nucleotide polymorphisms SNPs OAR2_14765360, OAR6_81718546, OAR11_62887032, OAR12_69606944 and OAR15_59871543 are associated with resistance and resilience traits in a flock of the Morada Nova sheep breed. Lambs were submitted to two consecutive parasite challenges by oral infection with 4000 infective larvae L3) of Haemonchus contortus. Fecal egg counts (FEC), packed cell volume (PVC) and body weight were measured every one or two weeks for 42 days in each trial. DNA samples from 287 lambs, 131 ewes and 4 rams were amplified by ARMS-PCR or PCR-RFLP and genotypes were determined. Analysis of variance (ANOVA) was used for association analyses between genotypes and phenotypes. In case of significant association, the allele substitution effect was calculated based on a linear model. OAR2_14765360 and OAR12_69606944 were associated with FEC, and OAR12_69606944 also had significant effects on PCV and weight gain, showing favourable associations of the CC genotype with all evaluated traits. Both OAR6_81718546 and OAR11_62887032 were associated with weight gain, and OAR6_81718546 had an additional effect on PCV. OAR15_59871543 was not polymorphic in the population. OAR6_81718546 and OAR12_69606944 presented significant allele substitution effects of -1.06 ± 0.52 kg for the T allele on final body weight and 0.74 ± 0.32 for the C allele in PCV of the same sampling date, respectively. This is the first report of SNPs associated with gastrointestinal nematode resistance in this sheep breed. Our findings support the existence of quantitatice trait loci (QTL) for resistance and resilience in linkage disequilibrium with the polymorphic SNPs and suggest their future use for explorations of these traits in Morada Nova sheep. MenosGastrointestinal nematodes are a major constraint in sheep production. Breeding for resistance has proven to be an effective and feasible approach to address this problem. The use and investigation of genetic markers for resistance traits could accelerate genetic progress and lead to a better understanding of underlying molecular mechanisms. Thus, the aim of this study was to evaluate if five single nucleotide polymorphisms SNPs OAR2_14765360, OAR6_81718546, OAR11_62887032, OAR12_69606944 and OAR15_59871543 are associated with resistance and resilience traits in a flock of the Morada Nova sheep breed. Lambs were submitted to two consecutive parasite challenges by oral infection with 4000 infective larvae L3) of Haemonchus contortus. Fecal egg counts (FEC), packed cell volume (PVC) and body weight were measured every one or two weeks for 42 days in each trial. DNA samples from 287 lambs, 131 ewes and 4 rams were amplified by ARMS-PCR or PCR-RFLP and genotypes were determined. Analysis of variance (ANOVA) was used for association analyses between genotypes and phenotypes. In case of significant association, the allele substitution effect was calculated based on a linear model. OAR2_14765360 and OAR12_69606944 were associated with FEC, and OAR12_69606944 also had significant effects on PCV and weight gain, showing favourable associations of the CC genotype with all evaluated traits. Both OAR6_81718546 and OAR11_62887032 were associated with weight gain, and OAR6_81718546 had an... Mostrar Tudo |
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Palavras-Chave: |
Host resistance; Morada Nova sheep; Parasite challenge; SNPs. |
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Thesaurus NAL: |
Genetic markers. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
Marc: |
LEADER 03156naa a2200325 a 4500
001 2121271
005 2020-04-20
008 2020 bl uuuu u00u1 u #d
022 $a0304-4017
024 7 $ahttps://doi.org/10.1016/j.vetpar.2020.109053$2DOI
100 1 $aHAEHLING, M. B. V.
245 $aFour single nucleotide polymorphisms (SNPs) are associated with resistance and resilience to Haemonchus contortus in Brazilian Morada Nova sheep.$h[electronic resource]
260 $c2020
300 $a11 p.
520 $aGastrointestinal nematodes are a major constraint in sheep production. Breeding for resistance has proven to be an effective and feasible approach to address this problem. The use and investigation of genetic markers for resistance traits could accelerate genetic progress and lead to a better understanding of underlying molecular mechanisms. Thus, the aim of this study was to evaluate if five single nucleotide polymorphisms SNPs OAR2_14765360, OAR6_81718546, OAR11_62887032, OAR12_69606944 and OAR15_59871543 are associated with resistance and resilience traits in a flock of the Morada Nova sheep breed. Lambs were submitted to two consecutive parasite challenges by oral infection with 4000 infective larvae L3) of Haemonchus contortus. Fecal egg counts (FEC), packed cell volume (PVC) and body weight were measured every one or two weeks for 42 days in each trial. DNA samples from 287 lambs, 131 ewes and 4 rams were amplified by ARMS-PCR or PCR-RFLP and genotypes were determined. Analysis of variance (ANOVA) was used for association analyses between genotypes and phenotypes. In case of significant association, the allele substitution effect was calculated based on a linear model. OAR2_14765360 and OAR12_69606944 were associated with FEC, and OAR12_69606944 also had significant effects on PCV and weight gain, showing favourable associations of the CC genotype with all evaluated traits. Both OAR6_81718546 and OAR11_62887032 were associated with weight gain, and OAR6_81718546 had an additional effect on PCV. OAR15_59871543 was not polymorphic in the population. OAR6_81718546 and OAR12_69606944 presented significant allele substitution effects of -1.06 ± 0.52 kg for the T allele on final body weight and 0.74 ± 0.32 for the C allele in PCV of the same sampling date, respectively. This is the first report of SNPs associated with gastrointestinal nematode resistance in this sheep breed. Our findings support the existence of quantitatice trait loci (QTL) for resistance and resilience in linkage disequilibrium with the polymorphic SNPs and suggest their future use for explorations of these traits in Morada Nova sheep.
650 $aGenetic markers
653 $aHost resistance
653 $aMorada Nova sheep
653 $aParasite challenge
653 $aSNPs
700 1 $aCRUVINEL, G. G.
700 1 $aTOSCANO, J. H. B.
700 1 $aGIRALDELO, L. A.
700 1 $aSANTOS, I. B. dos
700 1 $aESTEVES, S. N.
700 1 $aBENAVIDES, M. V.
700 1 $aBARIONI JUNIOR, W.
700 1 $aNICIURA, S. C. M.
700 1 $aCHAGAS, A. C. de S.
773 $tVeterinary Parasitology$gv. 279, 2020, 109053.
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