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| Registros recuperados : 190 | |
| 61. |  | NESHICH, G.; JARDINE, J. G.; BERNARDES, R.; MAZONI, I.; MANCINI, A. L.; SILVEIRA, C. da. "Cloud computation" na forma de serviços WEB para um banco de dados federativo STING_RDB. In: SIMPÓSIO SOBRE INOVAÇÃO E CRIATIVIDADE CIENTÍFICA NA EMBRAPA, 1., 2008, Brasília, DF. Comunicações Selecionadas. Brasília, DF: Embrapa, 2008. Não paginado.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 63. | | MELO, R. C.; MAZONI, I.; NESHICH, G.; SANTORO, M. M.; MEIRA JÚNIOR, W. Contacts as the key elements for comparing two protein structures. In: ANNUAL INTERNATIONAL CONFERENCE ON INTELLIGENT SYSTEMS FOR MOLECULAR BIOLOGY, 14.; ANNUAL AB3C CONFERENCE, 2., 2006, Fortaleza. Conference Program... Fortaleza: ISCB, 2006. Não paginado. ISMB, X-MEETING 2006. Poster I-6.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 65. | | SILVA, M. C. M. da; NESHICH, G.; CHRISPEELS, M.; HONIG, B.; SA, M. F. G. de. Molecular basis of the interactions between insect x-amylases and inhibitors. In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUIMICA E BIOLOGIA MOLECULAR, 28., 1999, Caxambu, MG. Programa e resumos. Caxambu: Sociedade Brasileira de Bioquimica e Biologia Molecular, 1999. p.35E-63.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 66. | | FERRAZ, L. F. C.; SOARDI, F.; FALCÃO, P.; NESHICH, G.; MELLO, M. P. de. Molecular homologous modeling of 3B-HSD2 mutant enzyme: structure-function aspects of Pro222GLN mutation correlates with the experimental data from a patient with congenital adrenal hyperplasia. In: ANNUAL INTERNATIONAL CONFERENCE ON INTELLIGENT SYSTEMS FOR MOLECULAR BIOLOGY, 14.; ANNUAL AB3C CONFERENCE, 2., 2006, Fortaleza. Conference Program... Fortaleza: ISCB, 2006. Não paginado. ISMB, X-MEETING 2006. Poster D-12.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 67. | | MARCELLINO, L. H.; NESHICH, G.; GROSSI DE SA, M. F.; KREBBERS, E.; GANDER, E. S. Modified 2S albumins with improved tryptophan content are correctly expressed in transgenic tobacco plants. FEBS Letters, Amsterdam, v.385, n.3, p.154-158, 1996. p.154-158| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 69. | | SALIM, J. A.; BORRO, L.; MAZONI, I.; YANO, I. H.; JARDINE, J. G.; NESHICH, G. Multiple structure single parameter: analysis of a single protein nano environment descriptor characterizing a shared loci on structurally aligned proteins. Bioinformatics, v. 32, n. 12, p. 1885-1887, 2016.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 72. | | MAZONI, I.; BORRO, L. C.; ALVARENGA, D.; JARDINE, J. G.; NESHICH, G. Studying structure function relationship of proteins using "remediated" PDB files. In: ANNUAL MEETING OF SBBQ, 37.; CONGRESS OF THE PAN-AMERICAN ASSOCIATION FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, 11, 2008, Águas de Lindóia. Program and index... Águas de Lindóia, Program and index... Águas de Lindóia: SBBq, 2008. Não paginado. PABMB.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 73. | | MAZONI, I.; BORRO, L. C.; JARDINE, J. G.; YANO, I. H.; SALIM, J. A.; NESHICH, G. Study of specific nanoenvironments containing [alfa]-helices in all-[alfa] and ([alfa]+[beta])+([alfa]/[beta]) proteins. Plos One, v. 13, n. 7, p. 1-25, 2018. Artigo e0200018.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Territorial. |
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| 79. | | FERNANDEZ, J. H.; MELLO, M. O; GALGARO, L.; TANAKA, A. S.; SILVA-FILHO, M. C.; NESHICH, G. Proteinase inhibition using small Bowman-Birk-type structures. Genetics and Molecular Research, v. 6, n. 4, p. 846-858, 2007.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 80. | | SANTOS, M. T.; FERRAZ, L. F. C.; REIS, F. C.; FALCÃO, P. R. K.; NESHICH, G.; OTTOBONI, L. M. M. Análise estrutural e funcional da proteína CMP quinase de Acidithiobacillus ferrooxidans. In: CONGRESSO BRASILEIRO DE GENÉTICA, 51., 2005, Águas de Lindóia. Resumos... Águas de Lindóias: SBG, 2005. p. 1075. Na publicação: Falcão, PK.| Biblioteca(s): Embrapa Agricultura Digital. |
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| Registros recuperados : 190 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Meio Ambiente. |
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Data corrente: |
20/04/2006 |
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Data da última atualização: |
31/08/2015 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
JESUS, K. R. E. de; FALCAO, P. R. K.; NESHICH, G. |
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Afiliação: |
KATIA REGINA EVARISTO DE JESUS, CNPMA; PAULA REGINA KUSER FALCAO, CNPTIA; GORAN NESHICH, CNPTIA. |
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Título: |
Structural analyses of CRY 1Ac protein from Bacillus thuringiensis. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
In: X-MEETING INTERNATIONAL CONFERENCE OF THE AB3C, 1., 2005, Caxambu/MG. Presented posters. Caxambu/MG: Associação Brasileira de Bioinformática e Biologia Computacional, 2005. p. 133. |
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Idioma: |
Inglês
Português |
|
Conteúdo: |
Cry protein is a delta endotoxin of the Bacillus family that provides an entomopatogenic activity to Bacilius thuringiensis. These proteins have a specific toxic activity against three types of insect larva: Lepdopthera, Diptera and Coleopthera. The insecticidal toxins are produced during spore formation. When an insect ingests these proteins they are activated by proteolytic cleavage. The toxin, after the ingestion, is solubitized by the alcalin pH in the digestive tract of the target insect. Once activated the endotoxin binds to the gut epithelium and causes cell lysis leading to death. These proteins are the active agents used in the majority of biorational pesticides and insect-resistant transgenic crops. This activated region of the delta endotoxin is composed of three structural domains. Domain I is involved in membrane insertion, pore formation and toxicity. The second and third domains are involved in receptor binding and specifically domain III is important in insect specificity. There are around 120 sequences of Cry toxins, and only five structures were deposited on the Protein Data Bank (PDB). There is a large interest in the toxin Cry 1Ac because it is commonly used to create transgenic plants with insect resistance. A theoretical model of the Cry lAc toxin was obtained on the basis of the coordinates of the insecticidal protein Cry lAa (PDB code:lciy.pdb) [1] as a template. The high sequence identity (73%) and a good correlation coefficient obtained from the elctron density server [2] indicates that 1ciy structure could be used as a template. The model corresponds to residues 33-618 of the primary structure and consists of domains I, II and III. The sequence of domain I is highly conserved, while domain II and III have the lower sequence similarity. The 3D model was construct using Modeller v7.7 [3]. Aimed at verifying whether the amino acid differences in domains II and III could be responsible for insect specifícity, this two structures were structurally aligned and compared with the Sting protein structure analysis program. Knowing that each amino acid substitution could potentially affect the protein structure and function, these amino acids were mapped and evaluated according to: conservation, change in solvent accessibility, side-chain volume change, effect on amino-acid interactions, protein electrostatics, and physicochemical properties of amino acids. Such approach can open a new insight into understanding of specificity that Cry protein has for receptors in plant pests and can help in the design of mutagenesis experiments aimed to elucidate the mechanism of action of the CrylAc toxin. MenosCry protein is a delta endotoxin of the Bacillus family that provides an entomopatogenic activity to Bacilius thuringiensis. These proteins have a specific toxic activity against three types of insect larva: Lepdopthera, Diptera and Coleopthera. The insecticidal toxins are produced during spore formation. When an insect ingests these proteins they are activated by proteolytic cleavage. The toxin, after the ingestion, is solubitized by the alcalin pH in the digestive tract of the target insect. Once activated the endotoxin binds to the gut epithelium and causes cell lysis leading to death. These proteins are the active agents used in the majority of biorational pesticides and insect-resistant transgenic crops. This activated region of the delta endotoxin is composed of three structural domains. Domain I is involved in membrane insertion, pore formation and toxicity. The second and third domains are involved in receptor binding and specifically domain III is important in insect specificity. There are around 120 sequences of Cry toxins, and only five structures were deposited on the Protein Data Bank (PDB). There is a large interest in the toxin Cry 1Ac because it is commonly used to create transgenic plants with insect resistance. A theoretical model of the Cry lAc toxin was obtained on the basis of the coordinates of the insecticidal protein Cry lAa (PDB code:lciy.pdb) [1] as a template. The high sequence identity (73%) and a good correlation coefficient obtained from the elc... Mostrar Tudo |
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Palavras-Chave: |
Bioinformática. |
|
Thesagro: |
Bacillus thuringiensis; Proteina. |
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Thesaurus NAL: |
Bioinformatics; Proteins. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/127545/1/2005RA-078.pdf
|
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Marc: |
LEADER 03359nam a2200193 a 4500
001 2021515
005 2015-08-31
008 2005 bl uuuu u00u1 u #d
100 1 $aJESUS, K. R. E. de
245 $aStructural analyses of CRY 1Ac protein from Bacillus thuringiensis.$h[electronic resource]
260 $aIn: X-MEETING INTERNATIONAL CONFERENCE OF THE AB3C, 1., 2005, Caxambu/MG. Presented posters. Caxambu/MG: Associação Brasileira de Bioinformática e Biologia Computacional, 2005. p. 133.$c2005
520 $aCry protein is a delta endotoxin of the Bacillus family that provides an entomopatogenic activity to Bacilius thuringiensis. These proteins have a specific toxic activity against three types of insect larva: Lepdopthera, Diptera and Coleopthera. The insecticidal toxins are produced during spore formation. When an insect ingests these proteins they are activated by proteolytic cleavage. The toxin, after the ingestion, is solubitized by the alcalin pH in the digestive tract of the target insect. Once activated the endotoxin binds to the gut epithelium and causes cell lysis leading to death. These proteins are the active agents used in the majority of biorational pesticides and insect-resistant transgenic crops. This activated region of the delta endotoxin is composed of three structural domains. Domain I is involved in membrane insertion, pore formation and toxicity. The second and third domains are involved in receptor binding and specifically domain III is important in insect specificity. There are around 120 sequences of Cry toxins, and only five structures were deposited on the Protein Data Bank (PDB). There is a large interest in the toxin Cry 1Ac because it is commonly used to create transgenic plants with insect resistance. A theoretical model of the Cry lAc toxin was obtained on the basis of the coordinates of the insecticidal protein Cry lAa (PDB code:lciy.pdb) [1] as a template. The high sequence identity (73%) and a good correlation coefficient obtained from the elctron density server [2] indicates that 1ciy structure could be used as a template. The model corresponds to residues 33-618 of the primary structure and consists of domains I, II and III. The sequence of domain I is highly conserved, while domain II and III have the lower sequence similarity. The 3D model was construct using Modeller v7.7 [3]. Aimed at verifying whether the amino acid differences in domains II and III could be responsible for insect specifícity, this two structures were structurally aligned and compared with the Sting protein structure analysis program. Knowing that each amino acid substitution could potentially affect the protein structure and function, these amino acids were mapped and evaluated according to: conservation, change in solvent accessibility, side-chain volume change, effect on amino-acid interactions, protein electrostatics, and physicochemical properties of amino acids. Such approach can open a new insight into understanding of specificity that Cry protein has for receptors in plant pests and can help in the design of mutagenesis experiments aimed to elucidate the mechanism of action of the CrylAc toxin.
650 $aBioinformatics
650 $aProteins
650 $aBacillus thuringiensis
650 $aProteina
653 $aBioinformática
700 1 $aFALCAO, P. R. K.
700 1 $aNESHICH, G.
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Embrapa Meio Ambiente (CNPMA) |
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