Data/hora: 11/06/2024 22:42:36

Biblioteca(s): | Embrapa Soja.
Data corrente: | 08/03/2005
Data da última atualização: | 11/07/2017
Autoria: | BINNECK, E.; SILVA, J. F. V.; NEUMAIER, N.; FARIAS, J. R. B.; NEPOMUCENO, A. L.
Afiliação: | ELISEU BINNECK, CNPSO; JOAO FLAVIO VELOSO SILVA, GPR; NORMAN NEUMAIER, CNPSO; JOSE RENATO BOUCAS FARIAS, CNPSO; ALEXANDRE LIMA NEPOMUCENO, CNPSO.
Título: | VSQual: a visual system to assist DNA sequencing quality control.
Ano de publicação: | 2004
Fonte/Imprenta: | Genetics and Molecular Research, Ribeirão Preto, v. 3, n. 4, p. 474-482, 2004.
Idioma: | Inglês
Conteúdo: | A lack of plant software tools that support small- to medium-scale DNA sequencing efforts is a major hindrance for recording and using laboratory workflow information to monitor the overall quality of data production. Here we describe VSQual, a set of Perl programs intended to provide simple and powerful tools to check several quality features of the sequencing data generated by automated DNA sequencing machines. The core program of VSQual is a flexible Perl-based pipeline, designed to be accessible and useful for both programmers and non-programmers. This pipeline directs the processing steps and can be easily customized for laboratory needs. Basically, the raw DNA sequencing trace files are processed by Phred and Cross_match, then the outputs are parsed, reformatted into Web-based graphical reports, and added to a Web site structure. The result is a set of real time sequencing reports easily accessible and understood by common laboratory people. These reports facilitate the monitoring of DNA sequencing as well as the management of laboratory workflow, significantly reducing operational costs and ensuring high quality and scientifically reliable results.
Palavras-Chave: | Bioinformática.
Categoria do assunto: | --
Registro original: | Embrapa Soja (CNPSO)

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