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| 2. |  | MARTINS, L. H. da S.; MOREIRA NETO, J.; LOPES, A. S.; RODRIGUES, A. M. da C.; CARVALHO, A. V.; OLIVEIRA, J. A. R. de; MOREIRA, D. K. T. Study of preparation, composition and moisture sorption isotherm of Amazon River shrimp meal. LWT - Food Science and Technology, v. 79, p. 376-383, June 2017.| Biblioteca(s): Embrapa Amazônia Oriental. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
11/04/2000 |
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Data da última atualização: |
15/10/2004 |
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Autoria: |
NEPOMUCENO, A. L.; NEUMAIER, N.; FARIAS, J. R. B.; CASAGRANDE, E. |
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Título: |
Gene expression in brazilian soybean genotypes tolerant to water deficit. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 6., 1999, Chicago. Proceedings: invited and contributed papers and posters. Chicago: University of Illinois / Soybean Research & Development Council, 1999. |
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Páginas: |
p.439. |
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Idioma: |
Inglês |
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Conteúdo: |
Abiotic stresses such as drought can significantly reduce crop yields and restrict the areas on which crops can be cultivated. The implications of this are tremendous since not only are producers affected but the entire society also. The development of new cultivars more tolerant to water deficits is one of the options to attenuate this problem. However, tolerance to drought in plants clearly is not a simple trait, but a complex of mechanisms working in combination to avoid or to tolerate water deficits. All mechanisms that plants use to tolerate drought do have a molecular/genetic basis. Comparison of gene expression in different genotypes and treatments should provide underlying information needed to analyze the biological processes that control how organisms respond to stress situations. This work has been conducted with the main objective of identifying, cloning and sequencing genes differentially expressed during water deficit in Brazilian soybean genotypes tolerant to drought. Physiological characterization results showed that cultivars BR-4 and OCEPAR 4 have a higher tolerance for periods of drought when compared with cultivars BR-16 and BRAGG. These four soybean genotypes are now been analyzed with the use of Differential Display (DD) a technique that uses mRNA as template to obtain representative cDNAs by reverse transcription and polymerase chain reaction. Differentially expressed cDNAs are extracted from the gel, cloned and sequenced. Earlier work conducted with cotton identified 52 mRNA transcripts differentially expressed during drought. Some of these transcript sequences were transformed in probes to survey Embrapa soybean germplasm bank to see if transcripts of these same gene families are expressed in soybean during water deficit. The differentially expressed mRNA transcripts identified in soybean can also be used as probes to screen germplasm. Plant physiological studies on responses to water deficit, molecular mapping of traits related to drought tolerance and the development of drought tolerant genotypes might be helped by these results. Furthermore, some of the identified genes may show a high potential for plant transformation studies. MenosAbiotic stresses such as drought can significantly reduce crop yields and restrict the areas on which crops can be cultivated. The implications of this are tremendous since not only are producers affected but the entire society also. The development of new cultivars more tolerant to water deficits is one of the options to attenuate this problem. However, tolerance to drought in plants clearly is not a simple trait, but a complex of mechanisms working in combination to avoid or to tolerate water deficits. All mechanisms that plants use to tolerate drought do have a molecular/genetic basis. Comparison of gene expression in different genotypes and treatments should provide underlying information needed to analyze the biological processes that control how organisms respond to stress situations. This work has been conducted with the main objective of identifying, cloning and sequencing genes differentially expressed during water deficit in Brazilian soybean genotypes tolerant to drought. Physiological characterization results showed that cultivars BR-4 and OCEPAR 4 have a higher tolerance for periods of drought when compared with cultivars BR-16 and BRAGG. These four soybean genotypes are now been analyzed with the use of Differential Display (DD) a technique that uses mRNA as template to obtain representative cDNAs by reverse transcription and polymerase chain reaction. Differentially expressed cDNAs are extracted from the gel, cloned and sequenced. Earlier work conducted with c... Mostrar Tudo |
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Palavras-Chave: |
Brasil; Soybean; Tolerancia a seca. |
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Thesagro: |
Soja. |
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Thesaurus NAL: |
Brazil; soil water deficit. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02965naa a2200241 a 4500
001 1461655
005 2004-10-15
008 1999 bl uuuu u00u1 u #d
100 1 $aNEPOMUCENO, A. L.
245 $aGene expression in brazilian soybean genotypes tolerant to water deficit.
260 $c1999
300 $ap.439.
520 $aAbiotic stresses such as drought can significantly reduce crop yields and restrict the areas on which crops can be cultivated. The implications of this are tremendous since not only are producers affected but the entire society also. The development of new cultivars more tolerant to water deficits is one of the options to attenuate this problem. However, tolerance to drought in plants clearly is not a simple trait, but a complex of mechanisms working in combination to avoid or to tolerate water deficits. All mechanisms that plants use to tolerate drought do have a molecular/genetic basis. Comparison of gene expression in different genotypes and treatments should provide underlying information needed to analyze the biological processes that control how organisms respond to stress situations. This work has been conducted with the main objective of identifying, cloning and sequencing genes differentially expressed during water deficit in Brazilian soybean genotypes tolerant to drought. Physiological characterization results showed that cultivars BR-4 and OCEPAR 4 have a higher tolerance for periods of drought when compared with cultivars BR-16 and BRAGG. These four soybean genotypes are now been analyzed with the use of Differential Display (DD) a technique that uses mRNA as template to obtain representative cDNAs by reverse transcription and polymerase chain reaction. Differentially expressed cDNAs are extracted from the gel, cloned and sequenced. Earlier work conducted with cotton identified 52 mRNA transcripts differentially expressed during drought. Some of these transcript sequences were transformed in probes to survey Embrapa soybean germplasm bank to see if transcripts of these same gene families are expressed in soybean during water deficit. The differentially expressed mRNA transcripts identified in soybean can also be used as probes to screen germplasm. Plant physiological studies on responses to water deficit, molecular mapping of traits related to drought tolerance and the development of drought tolerant genotypes might be helped by these results. Furthermore, some of the identified genes may show a high potential for plant transformation studies.
650 $aBrazil
650 $asoil water deficit
650 $aSoja
653 $aBrasil
653 $aSoybean
653 $aTolerancia a seca
700 1 $aNEUMAIER, N.
700 1 $aFARIAS, J. R. B.
700 1 $aCASAGRANDE, E.
773 $tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 6., 1999, Chicago. Proceedings: invited and contributed papers and posters. Chicago: University of Illinois / Soybean Research & Development Council, 1999.
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