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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sul. Para informações adicionais entre em contato com cppsul.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sul. |
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Data corrente: |
23/01/2019 |
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Data da última atualização: |
23/01/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
NICIURA, S. C. M.; CRUVINEL, G. G.; MORAES, C. V.; DONATONI, F. A. B.; MALAGO JUNIOR, W.; BENAVIDES, M. V.; CHAGAS, A. C. de S. |
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Afiliação: |
SIMONE CRISTINA MEO NICIURA, CPPSE; Giovanna Gabrielle Cruvinel, UNICEP; Caroline Valério Moraes, UFSCar; FLAVIA ALINE BRESSANI DONATONI, CPPSE; WILSON MALAGO JUNIOR, CPPSE; MAGDA VIEIRA BENAVIDES, CPPSUL; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
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Título: |
PCR-based genotyping of SNP markers in sheep. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Molecular Biology Reports, v. 45, n. 4, p. 651-654, Aug. 2018. |
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DOI: |
https://doi.org/10.1007/s11033-018-4206-8 |
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Idioma: |
Inglês |
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Conteúdo: |
Single nucleotide polymorphisms (SNPs) are the main type of variation in genome, enabling them to be associated with traits of economic importance in livestock. Genome-wide association studies (GWAS) have led to the discovery of SNPs associated with desirable traits in sheep. However, in these studies, SNPs are genotyped by high-throughput methods in genome scale, which are expensive and require sophisticated equipment and analysis methods. Therefore, the goal of this study was to develop a reliable, rapid, and inexpensive polymerase chain reaction (PCR)-based method to genotype a medium number of animals for a few candidate SNPs previously associated with desirable phenotypes in sheep by GWAS, using markers associated with gastrointestinal nematode resistance as a model. DNA extracted from white-blood cells of 150 sheep was submitted to PCR amplification followed by agarose gel electrophoresis and determination of banding pattern. Tetra-primer ARMS-PCR was successfully optimized after changes in annealing temperature; annealing and extension times; concentration of MgCl2 and DNA; ratios of inner, outer, forward and reverse primer; and addition of adjuvants, for genotyping the OAR2_14765360, OAR6_81718546, OAR11_62887032, and OAR12_69606944 SNPs in sheep. An extensive optimization of tetra-primer ARMS-PCR resulted in a suitable, simple, cost-effective PCR-based method of genotyping four SNP markers previously detected by chip arrays. |
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Palavras-Chave: |
PCR RFLP; Resistência nematódeo gastrintestinal; Tetra primer ARMS PCR. |
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Thesagro: |
Marcador Molecular; Ovino. |
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Thesaurus Nal: |
Genome. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02277naa a2200277 a 4500
001 2104694
005 2019-01-23
008 2018 bl --- 0-- u #d
024 7 $ahttps://doi.org/10.1007/s11033-018-4206-8$2DOI
100 1 $aNICIURA, S. C. M.
245 $aPCR-based genotyping of SNP markers in sheep.$h[electronic resource]
260 $c2018
520 $aSingle nucleotide polymorphisms (SNPs) are the main type of variation in genome, enabling them to be associated with traits of economic importance in livestock. Genome-wide association studies (GWAS) have led to the discovery of SNPs associated with desirable traits in sheep. However, in these studies, SNPs are genotyped by high-throughput methods in genome scale, which are expensive and require sophisticated equipment and analysis methods. Therefore, the goal of this study was to develop a reliable, rapid, and inexpensive polymerase chain reaction (PCR)-based method to genotype a medium number of animals for a few candidate SNPs previously associated with desirable phenotypes in sheep by GWAS, using markers associated with gastrointestinal nematode resistance as a model. DNA extracted from white-blood cells of 150 sheep was submitted to PCR amplification followed by agarose gel electrophoresis and determination of banding pattern. Tetra-primer ARMS-PCR was successfully optimized after changes in annealing temperature; annealing and extension times; concentration of MgCl2 and DNA; ratios of inner, outer, forward and reverse primer; and addition of adjuvants, for genotyping the OAR2_14765360, OAR6_81718546, OAR11_62887032, and OAR12_69606944 SNPs in sheep. An extensive optimization of tetra-primer ARMS-PCR resulted in a suitable, simple, cost-effective PCR-based method of genotyping four SNP markers previously detected by chip arrays.
650 $aGenome
650 $aMarcador Molecular
650 $aOvino
653 $aPCR RFLP
653 $aResistência nematódeo gastrintestinal
653 $aTetra primer ARMS PCR
700 1 $aCRUVINEL, G. G.
700 1 $aMORAES, C. V.
700 1 $aDONATONI, F. A. B.
700 1 $aMALAGO JUNIOR, W.
700 1 $aBENAVIDES, M. V.
700 1 $aCHAGAS, A. C. de S.
773 $tMolecular Biology Reports$gv. 45, n. 4, p. 651-654, Aug. 2018.
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Registro original: |
Embrapa Pecuária Sul (CPPSUL) |
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
18/02/2013 |
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Data da última atualização: |
28/08/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
C - 0 |
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Autoria: |
NAUMOV, A.; OLIVEIRA, R. P. de; PRADO, R. B.; TURETTA, A. P. |
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Afiliação: |
Alexey Naumov, Lomonosov Moscow State University; RONALDO PEREIRA DE OLIVEIRA, CNPS; RACHEL BARDY PRADO, CNPS; ANA PAULA DIAS TURETTA, CNPS. |
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Título: |
Balanced fertilization for sustainable development of agriculture in the savannas of South America: a geographical approach. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Geography Environment Sustainability, v. 5, n. 4, p. 84-95, 2012. |
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DOI: |
https://doi.org/10.24057/2071-9388-2012-5-4-84-95 |
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Idioma: |
Inglês |
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Conteúdo: |
South American countries still possess considerable land reserves for agricultural development. Huge areas of virgin lands and natural pastures have been converted to agricultural crops and suffer increasing pressure. Growing demand for food in the world and attractiveness for capital investment drive this colonization further deep into the regions that cannot be converted to commercial agriculture without serious negative environmental consequences. Agricultural development in these areas neither can be considered economically sustainable, as high production costs sometimes surpass revenues from the harvest. Most of the recently colonized regions in South America are those under savanna landscapes, where low nutrient availability in the soil restricts agricultural use. Understanding the problem of sustainability of agricultural development in savannas is impossible without geographical analysis. Spatial approach at different scales enables precise vision of weak points of recently established agricultural systems and helps to draw solutions to diminish their instability. The objective of this paper was to develop, with GIS tools, a geographically based analysis that could identify the spatial variation and magnitude of soil potassium, potassium uptake, potassium fertilization, and potassium balance in order to improve the efficiency of mineral fertilizers use. |
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Palavras-Chave: |
Sustainable development of agriculture. |
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Thesagro: |
Cerrado. |
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Thesaurus NAL: |
Brazil; mineral fertilizers; savannas; South America. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/142502/1/gi412.pdf
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Marc: |
LEADER 02175naa a2200241 a 4500
001 1949679
005 2023-08-28
008 2012 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.24057/2071-9388-2012-5-4-84-95$2DOI
100 1 $aNAUMOV, A.
245 $aBalanced fertilization for sustainable development of agriculture in the savannas of South America$ba geographical approach.$h[electronic resource]
260 $c2012
520 $aSouth American countries still possess considerable land reserves for agricultural development. Huge areas of virgin lands and natural pastures have been converted to agricultural crops and suffer increasing pressure. Growing demand for food in the world and attractiveness for capital investment drive this colonization further deep into the regions that cannot be converted to commercial agriculture without serious negative environmental consequences. Agricultural development in these areas neither can be considered economically sustainable, as high production costs sometimes surpass revenues from the harvest. Most of the recently colonized regions in South America are those under savanna landscapes, where low nutrient availability in the soil restricts agricultural use. Understanding the problem of sustainability of agricultural development in savannas is impossible without geographical analysis. Spatial approach at different scales enables precise vision of weak points of recently established agricultural systems and helps to draw solutions to diminish their instability. The objective of this paper was to develop, with GIS tools, a geographically based analysis that could identify the spatial variation and magnitude of soil potassium, potassium uptake, potassium fertilization, and potassium balance in order to improve the efficiency of mineral fertilizers use.
650 $aBrazil
650 $amineral fertilizers
650 $asavannas
650 $aSouth America
650 $aCerrado
653 $aSustainable development of agriculture
700 1 $aOLIVEIRA, R. P. de
700 1 $aPRADO, R. B.
700 1 $aTURETTA, A. P.
773 $tGeography Environment Sustainability$gv. 5, n. 4, p. 84-95, 2012.
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