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41. | | FERREIRA, G. B.; ÁVILA, A. C. de; NAGATA, T.; INOUE-NAGATA, A. K. Characterization of two potato virus y isolates collected in tomato and pepper plants. Virus Reviews & Research, Florianópolis, v. 9, p. 250-251, 2004. Suplement 1. Resumo. Trabalho apresentado no 15º National Meeting of Virology, São Pedro, 2004. Biblioteca(s): Embrapa Hortaliças. |
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43. | | ROCHA, W. B.; NAGATA, T.; GIORDANO, L. B.; PEREIRA, W.; INOUE-NAGATA, A. K. Susceptibility of weeds to a tomato begomovirus. Virus: Reviews & Research, Florianópolis, v. 8, p. 187, set. 2003. Suplemento 1. Trabalho apresentado no 14. National Meeting of Virology, 2003, Florianópolis, SC, Brasil. Resumo. Biblioteca(s): Embrapa Hortaliças. |
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49. | | SILVA, J. M. F.; BLAWID, R.; NAGATA, T.; FAJARDO, T. V. M. In silico detection of a defective genomic RNA of Grapevine leafroll-associated virus 4 strain 5 in High-Throughput Sequencing data. In:Congress of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine (ICVG), 19., 2018, Santiago, Chile. Anais... santiago, Chile: ICGV, April, 2018. p. 162-163. Biblioteca(s): Embrapa Uva e Vinho. |
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50. | | LUCINDA, N.; ORÍLIO, A. F.; SILVA, A. K. F.; INOUE-NAGATA, A. K.; NAGATA, T. Sequence analysis of the 5' terminal genomic region of Arracacha mottle virus. Tropical Plant Pathology, Brasília, DF, v. 33, p. S291, ago. 2008. Suplemento. Resumo VIR 026. Trabalho apresentado no 41. Congresso Brasileiro de Fitopatologia, 41. Annual Meeting of the Brazilian Phytopathological Society, Belo Horizonte, 2008. Biblioteca(s): Embrapa Hortaliças. |
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51. | | SILVA, A. K.; ROCHA, W. B.; LUCINDA, N.; INOUE-NAGATA, A. K.; NAGATA, T. Sequence analysis of the region near to the 5' genomic end of Pepper yellow mosaic virus. Tropical Plant Pathology, Brasília, DF, v. 33, p. S291, ago. 2008. Suplemento. Resumo VIR 027. Trabalho apresentado no 41. Congresso Brasileiro de Fitopatologia, 41. Annual Meeting of the Brazilian Phytopathological Society, Belo Horizonte, 2008. Biblioteca(s): Embrapa Hortaliças. |
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52. | | SOUZA, T. T. C.; ALVES, D. M. T.; INOUE-NAGATA, A. K.; NAGATA, T. Sequence and transient expression of groel protein isolated from whitefly endosybiont for use of geminivirus control. Virus Reviews & Research, Florianópolis, v. 9, p. 241, 2004. Suplement 1. Resumos. Trabalho apresentado no 15º National Meeting of Virology, São Pedro, 2004. Biblioteca(s): Embrapa Hortaliças. |
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53. | | QUADROS, A. F. F.; KAUFFMANN, C. M.; BOARI, A. de J.; BLAWID, R.; NAGATA, T. Sequenciamento do genoma de um Cytorhabdovirus infectando patchouli. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 51., 2019, Recife. Anais... Brasília, DF: Sociedade Brasileira de Fitopatologia, 2019. p. 836. Biblioteca(s): Embrapa Amazônia Oriental. |
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55. | | TAVARES, M. L.; BLAWID, R.; NAGATA, T.; NAGATA, A. K. I. Immunostaining of root tissue of nicotiana benthamiana infected with pepper risngpot virus. Virus Reviews and Research, v. 20, n. 2, p. 137, 2016. Edição dos resumos do XXVII Brazilian Congress of Virology & XI Mercosur Meeting of Virology, Pirienópolis, GO, 2016. p. 141. Na publicação: Inoue Nagata, A. K. Resumo PIV 223. Biblioteca(s): Embrapa Hortaliças. |
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Registros recuperados : 220 | |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Hortaliças. |
Data corrente: |
05/12/2007 |
Data da última atualização: |
24/01/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
Internacional - A |
Autoria: |
FERREIRA, P. de T de O.; LEMOS, T. O.; NAGATA, T.; INOUE-NAGATA, A. K. |
Afiliação: |
PAULO DE TARSO DE OLIVEIRA FERREIRA; THAÍS OLIVEIRA LEMOS, UNIVERSIDADE CATÓLICA DE BRASÍLIA; TATSUYA NAGATA, UNIVERSIDADE CATÓLICA DE BRASÍLIA; ALICE KAZUKO INOUE NAGATA, CNPH. |
Título: |
One-step cloning approach for construction of agroinfectious begomovirus clones. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Journal of Virological Methods, Amsterdam, v. 147, n. 2, p. 351-354, Feb. 2008. |
ISSN: |
1879-0984 |
DOI: |
10.1016/j.jviromet.2007.10.001 |
Idioma: |
Inglês |
Conteúdo: |
Most begomoviruses have a bipartite genome containing two circular ssDNA segments (DNA-A and DNA-B). Routine infectious clone construction relies upon cloning of the whole genome, which is then subcloned as a tandem one-and-half or two genome- (containing two replication origins) cassette into a vector prior to agro-inoculation. The construction of cassettes containing two replication origins is, however, a time-consuming process. Here an improved method for rapid construction of agroinfectious begomovirus clones is described. Total DNA was extracted from a tomato plant infected with Tomato golden vein virus and viral ssDNA molecules were amplified using phi-29 bacteriophage polymerase. High molecular weight DNA was partially digested with a single cutting restriction endonuclease (BamHI) and DNA fragments containing genome dimers were ligated into pCAMBIA0380, and used to transform Escherichia coli cells. This transformation yielded clones containing either DNA-A or DNA-B dimers. One clone each was used to transform Agrobacterium tumefaciens cells. DNA-A and DNA-B transformants were mixed and inoculated into test plants. All inoculated plants (tomato and Nicotiana benthamiana) became infected, confirming the infectivity of the clones. This approach was proven to be extremely fast and useful for the production of infectious clones. |
Palavras-Chave: |
Cultivar Santa Clara. |
Thesagro: |
Agrobacterium Tumefaciens; Clonagem; Tomate. |
Thesaurus NAL: |
Begomovirus; Cloning (plants); Solanum lycopersicum. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02164naa a2200265 a 4500 001 1780604 005 2024-01-24 008 2008 bl uuuu u00u1 u #d 022 $a1879-0984 024 7 $a10.1016/j.jviromet.2007.10.001$2DOI 100 1 $aFERREIRA, P. de T de O. 245 $aOne-step cloning approach for construction of agroinfectious begomovirus clones.$h[electronic resource] 260 $c2008 520 $aMost begomoviruses have a bipartite genome containing two circular ssDNA segments (DNA-A and DNA-B). Routine infectious clone construction relies upon cloning of the whole genome, which is then subcloned as a tandem one-and-half or two genome- (containing two replication origins) cassette into a vector prior to agro-inoculation. The construction of cassettes containing two replication origins is, however, a time-consuming process. Here an improved method for rapid construction of agroinfectious begomovirus clones is described. Total DNA was extracted from a tomato plant infected with Tomato golden vein virus and viral ssDNA molecules were amplified using phi-29 bacteriophage polymerase. High molecular weight DNA was partially digested with a single cutting restriction endonuclease (BamHI) and DNA fragments containing genome dimers were ligated into pCAMBIA0380, and used to transform Escherichia coli cells. This transformation yielded clones containing either DNA-A or DNA-B dimers. One clone each was used to transform Agrobacterium tumefaciens cells. DNA-A and DNA-B transformants were mixed and inoculated into test plants. All inoculated plants (tomato and Nicotiana benthamiana) became infected, confirming the infectivity of the clones. This approach was proven to be extremely fast and useful for the production of infectious clones. 650 $aBegomovirus 650 $aCloning (plants) 650 $aSolanum lycopersicum 650 $aAgrobacterium Tumefaciens 650 $aClonagem 650 $aTomate 653 $aCultivar Santa Clara 700 1 $aLEMOS, T. O. 700 1 $aNAGATA, T. 700 1 $aINOUE-NAGATA, A. K. 773 $tJournal of Virological Methods, Amsterdam$gv. 147, n. 2, p. 351-354, Feb. 2008.
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