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 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
15/10/2015 |
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Data da última atualização: |
22/02/2016 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
SILVA, E. da; VELASQUEZ, E.; SANTOS, A.; BARTZ, M. L. C.; LAVELLE, P.; BROWN, G. G. |
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Afiliação: |
Elodie da Silva, Acadêmica UFPR; Elena Velasquez, Universidad Nacional de Colombia; Alessandra Santos, UFPR; Marie Luise Carolina Bartz, Universidade Positivo; Patrick Lavelle, Institut de Recherche pour le Développement; GEORGE GARDNER BROWN, CNPF. |
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Título: |
General indicator of soil quality in different land-use systems of South Brazil. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: CONGRESO INTERNACIONAL DE SERVICIOS ECOSISTÉMICOS EN LOS NEOTRÓPICOS, 4., 2015, Mar del Plata. De la investigación a la acción: libro de resúmenes. Mar del Plata: GEAP, 2015. |
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Páginas: |
5 p. |
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Descrição Física: |
Disponível online. |
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Idioma: |
Inglês |
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Notas: |
CISEN 4. |
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Conteúdo: |
Since the degradation of ecosystems is associated with an important loss of soil biodiversity, there is an urgent need for soil quality evaluation in order to identify and develop more sustainable prac-tices. A General Indicator of Soil Quality (GISQ) has been proposed by Velasquez et al. (2007) which combines a set of subindicators. These subindicators may also be considered as indicators of the performance of different ecosystem services (ES) such as soil structure and nutrient cycling. This study aimed to evaluate the impact of different land-use systems on soil quality in a Natural Reserve of South Brazil. Variables associated with soil diversity, fertility, physical properties and aggregate morphology were measured for each system. The highest GISQ average value was calcu-lated for the Forest (0.80), while this system also had the highest values for macrofauna, chemical and physical subindicators. PCA analysis showed a significant separation (p<0.001) of the Soybean crop from the others systems based on the lowest subindicators values. The GISQ reflected the pro-vision of soil ES, showing that better soil quality produces more ES. The methodology used can be applied extensively and allows monitoring of land-use changes through time. It could therefore gui-de the implementation of soil restoration technologies. |
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Palavras-Chave: |
Calidad del suelo; Indicador; Indicator; Land use system; Qualidade do solo; Servicio ecosistémico; Sistema de uso da terra. |
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Thesaurus Nal: |
soil quality. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02298nam a2200289 a 4500
001 2026517
005 2016-02-22
008 2015 bl uuuu u00u1 u #d
100 1 $aSILVA, E. da
245 $aGeneral indicator of soil quality in different land-use systems of South Brazil.$h[electronic resource]
260 $aIn: CONGRESO INTERNACIONAL DE SERVICIOS ECOSISTÉMICOS EN LOS NEOTRÓPICOS, 4., 2015, Mar del Plata. De la investigación a la acción: libro de resúmenes. Mar del Plata: GEAP$c2015
300 $a5 p.$cDisponível online.
500 $aCISEN 4.
520 $aSince the degradation of ecosystems is associated with an important loss of soil biodiversity, there is an urgent need for soil quality evaluation in order to identify and develop more sustainable prac-tices. A General Indicator of Soil Quality (GISQ) has been proposed by Velasquez et al. (2007) which combines a set of subindicators. These subindicators may also be considered as indicators of the performance of different ecosystem services (ES) such as soil structure and nutrient cycling. This study aimed to evaluate the impact of different land-use systems on soil quality in a Natural Reserve of South Brazil. Variables associated with soil diversity, fertility, physical properties and aggregate morphology were measured for each system. The highest GISQ average value was calcu-lated for the Forest (0.80), while this system also had the highest values for macrofauna, chemical and physical subindicators. PCA analysis showed a significant separation (p<0.001) of the Soybean crop from the others systems based on the lowest subindicators values. The GISQ reflected the pro-vision of soil ES, showing that better soil quality produces more ES. The methodology used can be applied extensively and allows monitoring of land-use changes through time. It could therefore gui-de the implementation of soil restoration technologies.
650 $asoil quality
653 $aCalidad del suelo
653 $aIndicador
653 $aIndicator
653 $aLand use system
653 $aQualidade do solo
653 $aServicio ecosistémico
653 $aSistema de uso da terra
700 1 $aVELASQUEZ, E.
700 1 $aSANTOS, A.
700 1 $aBARTZ, M. L. C.
700 1 $aLAVELLE, P.
700 1 $aBROWN, G. G.
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Registro original: |
Embrapa Florestas (CNPF) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Pecuária Sudeste. |
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Data corrente: |
24/06/2013 |
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Data da última atualização: |
12/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
MELO, P. de C.; FERREIRA, L. M.; NADER FILHO, A.; ZAFALON, L. F.; VICENTE, H. I. G.; SOUZA, V. de. |
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Afiliação: |
POLIANA DE CASTRO MELO, Universidade Estadual Paulista; LUCIANO MENEZES FERREIRA, Universidade Estadual Paulista; ANTONIO NADER FILHO, Universidade Estadual Paulista; LUIZ FRANCISCO ZAFALON, CPPSE; HINIG ISA GODOY VICENTE, Secretaria de Agricultura e Abastecimento do Estado de São Paulo; VIVIANE DE SOUZA, CNPC. |
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Título: |
Comparison of methods for the detection of biofilm formation by Staphylococcus aureus isolated from bovine subclinical mastitis. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, v. 44, n. 1, p. 119-124, 2013. |
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DOI: |
https://doi.org/10.1590/S1517-83822013005000031 |
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Idioma: |
Inglês
Português |
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Conteúdo: |
Abstract: Biofilm formation is considered to be a selective advantage for Staphylococcus aureus mastitis isolates by facilitating bacterial persistence in the udder. It requires attachment to mammary epithelium, proliferation and accumulation of cells in multilayers. The objective of this study was to determine the sensitivity and specificity of three techniques for the detection of S. aureus biofilm-positive strains. Two phenotypic tests, including growth on microtitre plates and Congo red agar, were compared with a PCR technique using 94 S. aureus strains obtained from cows with subclinical mastitis from two farms in the state of São Paulo. These strains were characterised by in vitro slime production on Congo red agar, biofilm formation on microtitre plates and the presence of the icaA and icaD genes. The results revealed that 85% of the isolates tested produced slime on the Congo red agar, 98.9% of the isolates produced biofilms in vitro by adhering to sterile 96-well "U" bottom polystyrene tissue culture plates, and 95.7% of the isolates carried the icaA and icaD genes. The results of the phenotypic tests for biofilm formation were compared with those of the molecular analysis, and the sensitivity and specificity of the Congo red agar test were 88.9% and 100%, respectively, while those of the microtitre plate test were 100% and 25%, respectively. When the phenotypic methods for the detection of biofilm producers, namely growth on microtitre plates and Congo red agar, were compared, the sensitivity and specificity were 86% and 100%, respectively. Therefore, growth on Congo red agar and the microtitre plate test are methods that could be used to determine whether an isolate has the potential for biofilm production. MenosAbstract: Biofilm formation is considered to be a selective advantage for Staphylococcus aureus mastitis isolates by facilitating bacterial persistence in the udder. It requires attachment to mammary epithelium, proliferation and accumulation of cells in multilayers. The objective of this study was to determine the sensitivity and specificity of three techniques for the detection of S. aureus biofilm-positive strains. Two phenotypic tests, including growth on microtitre plates and Congo red agar, were compared with a PCR technique using 94 S. aureus strains obtained from cows with subclinical mastitis from two farms in the state of São Paulo. These strains were characterised by in vitro slime production on Congo red agar, biofilm formation on microtitre plates and the presence of the icaA and icaD genes. The results revealed that 85% of the isolates tested produced slime on the Congo red agar, 98.9% of the isolates produced biofilms in vitro by adhering to sterile 96-well "U" bottom polystyrene tissue culture plates, and 95.7% of the isolates carried the icaA and icaD genes. The results of the phenotypic tests for biofilm formation were compared with those of the molecular analysis, and the sensitivity and specificity of the Congo red agar test were 88.9% and 100%, respectively, while those of the microtitre plate test were 100% and 25%, respectively. When the phenotypic methods for the detection of biofilm producers, namely growth on microtitre plates and Congo red agar, we... Mostrar Tudo |
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Palavras-Chave: |
Biofilms; Mastite bovina; Molecular analysis; Phenotypic; Phenotypic and molecular analysis. |
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Thesagro: |
Biofilme; Bovino; Doença animal; Estirpe; Mamite; Staphylococcus aureus. |
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Thesaurus NAL: |
Bovine mastitis; Mammary gland diseases. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/84964/1/PROCI-2013.00046.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/84668/1/API-Comparison-of-methods.pdf
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Marc: |
LEADER 02819naa a2200349 a 4500
001 1960826
005 2023-05-12
008 2013 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/S1517-83822013005000031$2DOI
100 1 $aMELO, P. de C.
245 $aComparison of methods for the detection of biofilm formation by Staphylococcus aureus isolated from bovine subclinical mastitis.$h[electronic resource]
260 $c2013
520 $aAbstract: Biofilm formation is considered to be a selective advantage for Staphylococcus aureus mastitis isolates by facilitating bacterial persistence in the udder. It requires attachment to mammary epithelium, proliferation and accumulation of cells in multilayers. The objective of this study was to determine the sensitivity and specificity of three techniques for the detection of S. aureus biofilm-positive strains. Two phenotypic tests, including growth on microtitre plates and Congo red agar, were compared with a PCR technique using 94 S. aureus strains obtained from cows with subclinical mastitis from two farms in the state of São Paulo. These strains were characterised by in vitro slime production on Congo red agar, biofilm formation on microtitre plates and the presence of the icaA and icaD genes. The results revealed that 85% of the isolates tested produced slime on the Congo red agar, 98.9% of the isolates produced biofilms in vitro by adhering to sterile 96-well "U" bottom polystyrene tissue culture plates, and 95.7% of the isolates carried the icaA and icaD genes. The results of the phenotypic tests for biofilm formation were compared with those of the molecular analysis, and the sensitivity and specificity of the Congo red agar test were 88.9% and 100%, respectively, while those of the microtitre plate test were 100% and 25%, respectively. When the phenotypic methods for the detection of biofilm producers, namely growth on microtitre plates and Congo red agar, were compared, the sensitivity and specificity were 86% and 100%, respectively. Therefore, growth on Congo red agar and the microtitre plate test are methods that could be used to determine whether an isolate has the potential for biofilm production.
650 $aBovine mastitis
650 $aMammary gland diseases
650 $aBiofilme
650 $aBovino
650 $aDoença animal
650 $aEstirpe
650 $aMamite
650 $aStaphylococcus aureus
653 $aBiofilms
653 $aMastite bovina
653 $aMolecular analysis
653 $aPhenotypic
653 $aPhenotypic and molecular analysis
700 1 $aFERREIRA, L. M.
700 1 $aNADER FILHO, A.
700 1 $aZAFALON, L. F.
700 1 $aVICENTE, H. I. G.
700 1 $aSOUZA, V. de
773 $tBrazilian Journal of Microbiology$gv. 44, n. 1, p. 119-124, 2013.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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