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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
22/02/2017 |
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Data da última atualização: |
22/02/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MILLER, A. M.; FIGUEIREDO, J. E. F.; LINDE, G. A.; COLAUTO, N. B.; PACOLA-MEIRELLES, L. D. |
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Afiliação: |
Universidade Estadual de Londrina; JOSE EDSON FONTES FIGUEIREDO, CNPMS; Universidade Paranaense; Universidade Paranaense; Universidade Estadual de Londrina. |
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Título: |
Characterization of the inaA gene and expression of ice nucleation phenotype in Pantoea ananatis isolates from Maize White Spot disease. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Genetics and Molecular Research, Ribeirão Preto, v. 15, n. 1, p. 1-8, 2016. |
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DOI: |
10.4238/gmr.15017863 |
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Idioma: |
Inglês |
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Conteúdo: |
Maize White Spot (MWS), a foliar disease caused by Pantoea ananatis, could cause up to 60% yield loss. Some strains of P. ananatis harboring the ice nucleation gene inaA catalyze the formation of ice nuclei, causing tissue damage at temperatures slightly below freezing. Little is known about the relationship between the presence of the ina gene in this maize pathogen and its expression during the phenomenon of ice nucleus formation. Here, we attempted to verify the presence of the inaA gene and the expression of phenotype in vitro. The identity of the isolates and the presence of the inaA gene were determined by P. ananatis speciesspecific primers. The expression of the inaA gene was assessed in vitro by the visualization of ice-crystal formation in water at subzero temperatures. A total of ninety P. ananatis isolates from MWS lesions were characterized. The presence of the inaA gene was confirmed by gel electrophoresis of the 350-400-bp PCR products. The inaA primers did not lead to DNA fragment amplification in three isolates. The ice nucleation phenotype was expressed in 83.34% of the isolates carrying the inaA gene. Our study showed that the ice nucleation in P. ananatis isolated from MWS lesions was dependent on the presence of a functional ina gene in the genome. We also found evidence indicating that some P. ananatis strains have a mutated form of the inaA gene, producing a non-functional ice nucleation protein. This is the first report on inaA gene characterization in P. ananatis isolates from Maize White Spot. MenosMaize White Spot (MWS), a foliar disease caused by Pantoea ananatis, could cause up to 60% yield loss. Some strains of P. ananatis harboring the ice nucleation gene inaA catalyze the formation of ice nuclei, causing tissue damage at temperatures slightly below freezing. Little is known about the relationship between the presence of the ina gene in this maize pathogen and its expression during the phenomenon of ice nucleus formation. Here, we attempted to verify the presence of the inaA gene and the expression of phenotype in vitro. The identity of the isolates and the presence of the inaA gene were determined by P. ananatis speciesspecific primers. The expression of the inaA gene was assessed in vitro by the visualization of ice-crystal formation in water at subzero temperatures. A total of ninety P. ananatis isolates from MWS lesions were characterized. The presence of the inaA gene was confirmed by gel electrophoresis of the 350-400-bp PCR products. The inaA primers did not lead to DNA fragment amplification in three isolates. The ice nucleation phenotype was expressed in 83.34% of the isolates carrying the inaA gene. Our study showed that the ice nucleation in P. ananatis isolated from MWS lesions was dependent on the presence of a functional ina gene in the genome. We also found evidence indicating that some P. ananatis strains have a mutated form of the inaA gene, producing a non-functional ice nucleation protein. This is the first report on inaA gene characterization i... Mostrar Tudo |
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Palavras-Chave: |
Bactérias fitopatogênicas; Doença foliar; Fenótipo INA; Variabilidade. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/156438/1/Characterization-inaA.pdf
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Marc: |
LEADER 02317naa a2200229 a 4500
001 2065205
005 2017-02-22
008 2016 bl uuuu u00u1 u #d
024 7 $a10.4238/gmr.15017863$2DOI
100 1 $aMILLER, A. M.
245 $aCharacterization of the inaA gene and expression of ice nucleation phenotype in Pantoea ananatis isolates from Maize White Spot disease.$h[electronic resource]
260 $c2016
520 $aMaize White Spot (MWS), a foliar disease caused by Pantoea ananatis, could cause up to 60% yield loss. Some strains of P. ananatis harboring the ice nucleation gene inaA catalyze the formation of ice nuclei, causing tissue damage at temperatures slightly below freezing. Little is known about the relationship between the presence of the ina gene in this maize pathogen and its expression during the phenomenon of ice nucleus formation. Here, we attempted to verify the presence of the inaA gene and the expression of phenotype in vitro. The identity of the isolates and the presence of the inaA gene were determined by P. ananatis speciesspecific primers. The expression of the inaA gene was assessed in vitro by the visualization of ice-crystal formation in water at subzero temperatures. A total of ninety P. ananatis isolates from MWS lesions were characterized. The presence of the inaA gene was confirmed by gel electrophoresis of the 350-400-bp PCR products. The inaA primers did not lead to DNA fragment amplification in three isolates. The ice nucleation phenotype was expressed in 83.34% of the isolates carrying the inaA gene. Our study showed that the ice nucleation in P. ananatis isolated from MWS lesions was dependent on the presence of a functional ina gene in the genome. We also found evidence indicating that some P. ananatis strains have a mutated form of the inaA gene, producing a non-functional ice nucleation protein. This is the first report on inaA gene characterization in P. ananatis isolates from Maize White Spot.
653 $aBactérias fitopatogênicas
653 $aDoença foliar
653 $aFenótipo INA
653 $aVariabilidade
700 1 $aFIGUEIREDO, J. E. F.
700 1 $aLINDE, G. A.
700 1 $aCOLAUTO, N. B.
700 1 $aPACOLA-MEIRELLES, L. D.
773 $tGenetics and Molecular Research, Ribeirão Preto$gv. 15, n. 1, p. 1-8, 2016.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
29/08/2011 |
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Data da última atualização: |
29/08/2011 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
FAGUNDES, P. R. R.; MAGALHÃES JUNIOR, A. M. de; MOURA NETO, F. P.; PETRINI, J. A.; SEVERO, A.; SILVA, M. G. da. |
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Afiliação: |
PAULO RICARDO REIS FAGUNDES, CPACT; ARIANO MARTINS DE MAGALHAES JUNIOR, CPACT; FRANCISCO PEREIRA MOURA NETO, CNPAF; JOSE ALBERTO PETRINI, CPACT; ALCIDES CRISTIANO MORAIS SEVERO, CPACT; MARCIO GONÇALVES DA SILVA, estagiário CPACT. |
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Título: |
Avaliação de cultivares de arroz irrigado da Embrapa no Rio Grande do Sul, safra 2009/10. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE ARROZ IRRIGADO, 7., 2011, Balneário Camboriú. Racionalizando recursos e ampliando oportunidades: anais. Itajaí: Epagri, 2011. |
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Volume: |
v. 1. |
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Páginas: |
p. 129-132. |
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Idioma: |
Português |
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Conteúdo: |
Anualmente, desenvolve-se um trabalho para acompanhar, avaliar e relatar o comportamento de cultivares de arroz irrigado da Embrapa, nas diferentes regiões de cultivo deste cereal no RS. Neste trabalho são relatados os resultados obtidos na safra 2009/10. |
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Palavras-Chave: |
Interação genótipo x ambiente; Rio Grande do Sul. |
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Thesagro: |
Arroz irrigado; Melhoramento genético vegetal; Oryza sativa; Variedade. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/40657/1/ft129.pdf
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Marc: |
LEADER 01151nam a2200265 a 4500
001 1899059
005 2011-08-29
008 2011 bl uuuu u00u1 u #d
100 1 $aFAGUNDES, P. R. R.
245 $aAvaliação de cultivares de arroz irrigado da Embrapa no Rio Grande do Sul, safra 2009/10.
260 $aIn: CONGRESSO BRASILEIRO DE ARROZ IRRIGADO, 7., 2011, Balneário Camboriú. Racionalizando recursos e ampliando oportunidades: anais. Itajaí: Epagri$c2011
300 $ap. 129-132. v. 1.
490 $vv. 1.
520 $aAnualmente, desenvolve-se um trabalho para acompanhar, avaliar e relatar o comportamento de cultivares de arroz irrigado da Embrapa, nas diferentes regiões de cultivo deste cereal no RS. Neste trabalho são relatados os resultados obtidos na safra 2009/10.
650 $aArroz irrigado
650 $aMelhoramento genético vegetal
650 $aOryza sativa
650 $aVariedade
653 $aInteração genótipo x ambiente
653 $aRio Grande do Sul
700 1 $aMAGALHÃES JUNIOR, A. M. de
700 1 $aMOURA NETO, F. P.
700 1 $aPETRINI, J. A.
700 1 $aSEVERO, A.
700 1 $aSILVA, M. G. da
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Embrapa Arroz e Feijão (CNPAF) |
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