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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
15/06/2021 |
Data da última atualização: |
15/09/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SIMÕES, L. M. S.; SANTOS, A. P. C.; BOTTINO, M. P.; LIMA, E. A.; FERNANDES, U. R.; ORLANDI, R. E.; RODRIGUES, S. A. D.; CAIXETA, F. M.; ALVES, N. G.; SOUZA, J. C.; QUINTAO, C. C. R.; CAMARGO, L. S. A.; DODE, M. A. N.; SALES, J. N. S. |
Afiliação: |
L. M. S. SIMÕES, UFLA; A. P. C. SANTOS, UFLA; M. P. BOTTINO, UFLA; E. A. LIMA, UFLA; U. R. FERNANDES, UFLA; R. E. ORLANDI, UFLA; S. A. D. RODRIGUES, UNB; F. M. CAIXETA, UNB; N. G. ALVES, UFLA; J. C. SOUZA, UFLA; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; MARGOT ALVES NUNES DODE, Cenargen; J. N. S. SALES, UFLA. |
Título: |
Nuclear maturation kinetics and in vitro fertilization of immature bovine oocytes injected into pre-ovulatory follicles. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Theriogenology, v. 166, p. 90-96, 2021. |
DOI: |
https://doi.org/10.1016/j.theriogenology.2020.12.021 |
Idioma: |
Inglês |
Conteúdo: |
The maturation kinetics and in vitro fertilization of immature bovine oocytes injected by the intrafollicular oocyte injection (IFOT) technique into pre-ovulatory follicles of previously synchronized cows were evaluated. In Experiment 1, grade I, II and III cumulus-oocyte complexes (COCs) were randomly distributed to one of three Groups: Matvitro22 (COCs matured in vitro for 22 h), MatFol20 and MatFol28 (COCs matured in vivo after being injected into a pre-ovulatory follicle of previously synchronized cows for 19.8 ± 0.1 h and 28.3 ± 0.1 h, respectively). Cows received 12.5 mg of LH (Lutropin, Bioniche, Canada) at the time of IFOT in the MatFol20 Group or 10 h after IFOT in the MatFol28 Group. MatFol20 and MatFol28 COCs were aspirated approximately 20 h after the LH injection for nuclear maturation kinetics and recovery rate assessment. In Experiment 2, grade I, II, and III COCs were randomly distributed into two Groups: Matvitro22 Group, COCs were matured and fertilized in vitro, and MatFol20 Group, COCs were matured as in the MatFol20 Group in Experiment 1, but COCs were fertilized in vitro. Putative zygotes were classified as fertilized, unfertilized or polyspermic. In Experiment 1, the recovery rate was lower (P < 0.001) in the MatFol20 Group (52.9%, 91/172) compared with MatFol28 (72.9%, 113/155). Rate of oocytes in germinal vesicle stage, metaphase I, anaphase I and telophase I were similar among Groups. However, oocytes matured in vivo for 28.3 h had lower rate of metaphase II (P ¼ 0.001) and greater rates of degenerated (P ¼ 0.001) and parthenogenetically activated (P ¼ 0.001) oocytes. In experiment 2, the rates of polyspermy and degenerated were similar between Groups. However, the rate of fertilized oocytes was greater (P ¼ 0.05) in oocytes in the MatFol20 Group. It is concluded that oocyte in vivo maturation for 19.8 h after IFOT does not compromise the nuclear maturation kinetics and increases in vitro fertilization rates. However, the extra 10 h of intra-follicular incubation time decreased oocyte viability. MenosThe maturation kinetics and in vitro fertilization of immature bovine oocytes injected by the intrafollicular oocyte injection (IFOT) technique into pre-ovulatory follicles of previously synchronized cows were evaluated. In Experiment 1, grade I, II and III cumulus-oocyte complexes (COCs) were randomly distributed to one of three Groups: Matvitro22 (COCs matured in vitro for 22 h), MatFol20 and MatFol28 (COCs matured in vivo after being injected into a pre-ovulatory follicle of previously synchronized cows for 19.8 ± 0.1 h and 28.3 ± 0.1 h, respectively). Cows received 12.5 mg of LH (Lutropin, Bioniche, Canada) at the time of IFOT in the MatFol20 Group or 10 h after IFOT in the MatFol28 Group. MatFol20 and MatFol28 COCs were aspirated approximately 20 h after the LH injection for nuclear maturation kinetics and recovery rate assessment. In Experiment 2, grade I, II, and III COCs were randomly distributed into two Groups: Matvitro22 Group, COCs were matured and fertilized in vitro, and MatFol20 Group, COCs were matured as in the MatFol20 Group in Experiment 1, but COCs were fertilized in vitro. Putative zygotes were classified as fertilized, unfertilized or polyspermic. In Experiment 1, the recovery rate was lower (P < 0.001) in the MatFol20 Group (52.9%, 91/172) compared with MatFol28 (72.9%, 113/155). Rate of oocytes in germinal vesicle stage, metaphase I, anaphase I and telophase I were similar among Groups. However, oocytes matured in vivo for 28.3 h had lower rate of met... Mostrar Tudo |
Palavras-Chave: |
Fertilização in-vitro; IFOT; Intra-follicular. |
Thesagro: |
Bovino; Fertilidade Animal. |
Thesaurus Nal: |
Embryo (animal). |
Categoria do assunto: |
-- L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 03144naa a2200361 a 4500 001 2133325 005 2021-09-15 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.theriogenology.2020.12.021$2DOI 100 1 $aSIMÕES, L. M. S. 245 $aNuclear maturation kinetics and in vitro fertilization of immature bovine oocytes injected into pre-ovulatory follicles.$h[electronic resource] 260 $c2021 520 $aThe maturation kinetics and in vitro fertilization of immature bovine oocytes injected by the intrafollicular oocyte injection (IFOT) technique into pre-ovulatory follicles of previously synchronized cows were evaluated. In Experiment 1, grade I, II and III cumulus-oocyte complexes (COCs) were randomly distributed to one of three Groups: Matvitro22 (COCs matured in vitro for 22 h), MatFol20 and MatFol28 (COCs matured in vivo after being injected into a pre-ovulatory follicle of previously synchronized cows for 19.8 ± 0.1 h and 28.3 ± 0.1 h, respectively). Cows received 12.5 mg of LH (Lutropin, Bioniche, Canada) at the time of IFOT in the MatFol20 Group or 10 h after IFOT in the MatFol28 Group. MatFol20 and MatFol28 COCs were aspirated approximately 20 h after the LH injection for nuclear maturation kinetics and recovery rate assessment. In Experiment 2, grade I, II, and III COCs were randomly distributed into two Groups: Matvitro22 Group, COCs were matured and fertilized in vitro, and MatFol20 Group, COCs were matured as in the MatFol20 Group in Experiment 1, but COCs were fertilized in vitro. Putative zygotes were classified as fertilized, unfertilized or polyspermic. In Experiment 1, the recovery rate was lower (P < 0.001) in the MatFol20 Group (52.9%, 91/172) compared with MatFol28 (72.9%, 113/155). Rate of oocytes in germinal vesicle stage, metaphase I, anaphase I and telophase I were similar among Groups. However, oocytes matured in vivo for 28.3 h had lower rate of metaphase II (P ¼ 0.001) and greater rates of degenerated (P ¼ 0.001) and parthenogenetically activated (P ¼ 0.001) oocytes. In experiment 2, the rates of polyspermy and degenerated were similar between Groups. However, the rate of fertilized oocytes was greater (P ¼ 0.05) in oocytes in the MatFol20 Group. It is concluded that oocyte in vivo maturation for 19.8 h after IFOT does not compromise the nuclear maturation kinetics and increases in vitro fertilization rates. However, the extra 10 h of intra-follicular incubation time decreased oocyte viability. 650 $aEmbryo (animal) 650 $aBovino 650 $aFertilidade Animal 653 $aFertilização in-vitro 653 $aIFOT 653 $aIntra-follicular 700 1 $aSANTOS, A. P. C. 700 1 $aBOTTINO, M. P. 700 1 $aLIMA, E. A. 700 1 $aFERNANDES, U. R. 700 1 $aORLANDI, R. E. 700 1 $aRODRIGUES, S. A. D. 700 1 $aCAIXETA, F. M. 700 1 $aALVES, N. G. 700 1 $aSOUZA, J. C. 700 1 $aQUINTAO, C. C. R. 700 1 $aCAMARGO, L. S. A. 700 1 $aDODE, M. A. N. 700 1 $aSALES, J. N. S. 773 $tTheriogenology$gv. 166, p. 90-96, 2021.
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Embrapa Gado de Leite (CNPGL) |
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Biblioteca(s): |
Embrapa Clima Temperado. |
Data corrente: |
15/03/2017 |
Data da última atualização: |
05/04/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
MOURA, G. C. de; VIZZOTTO, M.; PICOLOTTO, L.; ANTUNES, L. E. C. |
Afiliação: |
GISELY CORREA DE MOURA; MARCIA VIZZOTTO, CPACT; LUCIONA PICOLOTTO; LUIS EDUARDO CORREA ANTUNES, CPACT. |
Título: |
Produção, qualidade físico-químico e compostos bioativos de frutos de mirtileiro misty sob diferentes intensidades de poda. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Revista Brasileira de Fruticultura, v. 39, p. 1-6, 2017. |
Idioma: |
Português |
Palavras-Chave: |
Funcionais; Qualidade de fruto. |
Thesagro: |
Manejo. |
Thesaurus NAL: |
Vaccinium. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/157672/1/Marcia-Vizzotto-02.14.01.019.00.04.008-Intensidade-de-poda-RBF.pdf
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Marc: |
LEADER 00619naa a2200193 a 4500 001 2067079 005 2018-04-05 008 2017 bl uuuu u00u1 u #d 100 1 $aMOURA, G. C. de 245 $aProdução, qualidade físico-químico e compostos bioativos de frutos de mirtileiro misty sob diferentes intensidades de poda.$h[electronic resource] 260 $c2017 650 $aVaccinium 650 $aManejo 653 $aFuncionais 653 $aQualidade de fruto 700 1 $aVIZZOTTO, M. 700 1 $aPICOLOTTO, L. 700 1 $aANTUNES, L. E. C. 773 $tRevista Brasileira de Fruticultura$gv. 39, p. 1-6, 2017.
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