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Registro Completo |
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
05/03/2004 |
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Data da última atualização: |
03/07/2008 |
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Autoria: |
SANTOS, J. R. M.; REIS, A.; BOITEUX, L. S.; GIORDANO, L. B. |
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Afiliação: |
Embrapa Hortaliças, Brasília, DF. |
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Título: |
Fontes de resistência genética múltipla a Fusarium oxysporum F. sp. lycopersici raça 2 e Verticillium dahliae raça 2 em germoplasma de Lycopersicon. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Summa Phytopathologica, Botucatu, v. 30, n. 1, p. 110-111, jan./mar. 2004. |
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Idioma: |
Inglês |
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Notas: |
Resumo. Trabalho apresentado no 27º Congresso Paulista de Fitopatologia, 2003, Botucatu. |
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Palavras-Chave: |
Lycopersicon; Verticillium dahhae. |
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Thesagro: |
Doença; Fusarium Oxysporum Lycopersici; Germoplasma; Resistência. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00824naa a2200229 a 4500
001 1775429
005 2008-07-03
008 2004 bl uuuu u00u1 u #d
100 1 $aSANTOS, J. R. M.
245 $aFontes de resistência genética múltipla a Fusarium oxysporum F. sp. lycopersici raça 2 e Verticillium dahliae raça 2 em germoplasma de Lycopersicon.
260 $c2004
500 $aResumo. Trabalho apresentado no 27º Congresso Paulista de Fitopatologia, 2003, Botucatu.
650 $aDoença
650 $aFusarium Oxysporum Lycopersici
650 $aGermoplasma
650 $aResistência
653 $aLycopersicon
653 $aVerticillium dahhae
700 1 $aREIS, A.
700 1 $aBOITEUX, L. S.
700 1 $aGIORDANO, L. B.
773 $tSumma Phytopathologica, Botucatu$gv. 30, n. 1, p. 110-111, jan./mar. 2004.
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Registro original: |
Embrapa Hortaliças (CNPH) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Amazônia Ocidental; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
04/10/2011 |
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Data da última atualização: |
05/02/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
PROSDOCIMI, P; BITTENCOURT, D.; SILVA, F. R. da; KIRST, M.; MOTA, P. C.; RECH, E. L. |
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Afiliação: |
FRANCISCO PROSDOCIMI, UFRJ, UCB; DANIELA MATIAS DE C BITTENCOURT, CPAA; FELIPE RODRIGUES DA SILVA, CNPTIA; MATIAS KIRST, University of Florida; PAULO C. MOTA, UnB; ELIBIO LEOPOLDO RECH FILHO, CENARGEN. |
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Título: |
Spinning gland transcriptomics from two main Clades of Spiders (Order: Araneae) - insights on their molecular, anatomical and behavioral evolution. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Plos One, San Francisco, v. 6, n. 6, p. 1-15, June 2011. |
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DOI: |
10.1371/journal.pone.0021634 |
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Idioma: |
Inglês |
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Conteúdo: |
Characterized by distinctive evolutionary adaptations, spiders provide a comprehensive system for evolutionary and developmental studies of anatomical organs, including silk and venom production. Here we performed cDNA sequencing using massively parallel sequencers (454 GS-FLX Titanium) to generate ~80,000 reads from the spinning gland of Actinopus spp. (infraorder: Mygalomorphae) and Gasteracantha cancriformis (infraorder: Araneomorphae, Orbiculariae clade). Actinopus spp. retains primitive characteristics on web usage and presents a single undifferentiated spinning gland while the orbiculariae spiders have seven differentiated spinning glands and complex patterns of web usage. MIRA, Celera Assembler and CAP3 software were used to cluster NGS reads for each spider. CAP3 unigenes passed through a pipeline for automatic annotation, classification by biological function, and comparative transcriptomics. Genes related to spider silks were manually curated and analyzed. Although a single spidroin gene family was found in Actinopus spp., a vast repertoire of specialized spider silk proteins was encountered in orbiculariae. Astacin-like metalloproteases (meprin subfamily) were shown to be some of the most sampled unigenes and duplicated gene families in G. cancriformis since its evolutionary split from mygalomorphs. Our results confirm that the evolution of the molecular repertoire of silk proteins was accompanied by the (i) anatomical differentiation of spinning glands and (ii) behavioral complexification in the web usage. Finally, a phylogenetic tree was constructed to cluster most of the known spidroins in gene clades. This is the first large-scale, multi-organism transcriptome for spider spinning glands and a first step into a broad understanding of spider web systems biology and evolution. MenosCharacterized by distinctive evolutionary adaptations, spiders provide a comprehensive system for evolutionary and developmental studies of anatomical organs, including silk and venom production. Here we performed cDNA sequencing using massively parallel sequencers (454 GS-FLX Titanium) to generate ~80,000 reads from the spinning gland of Actinopus spp. (infraorder: Mygalomorphae) and Gasteracantha cancriformis (infraorder: Araneomorphae, Orbiculariae clade). Actinopus spp. retains primitive characteristics on web usage and presents a single undifferentiated spinning gland while the orbiculariae spiders have seven differentiated spinning glands and complex patterns of web usage. MIRA, Celera Assembler and CAP3 software were used to cluster NGS reads for each spider. CAP3 unigenes passed through a pipeline for automatic annotation, classification by biological function, and comparative transcriptomics. Genes related to spider silks were manually curated and analyzed. Although a single spidroin gene family was found in Actinopus spp., a vast repertoire of specialized spider silk proteins was encountered in orbiculariae. Astacin-like metalloproteases (meprin subfamily) were shown to be some of the most sampled unigenes and duplicated gene families in G. cancriformis since its evolutionary split from mygalomorphs. Our results confirm that the evolution of the molecular repertoire of silk proteins was accompanied by the (i) anatomical differentiation of spinning glands and (ii) b... Mostrar Tudo |
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Palavras-Chave: |
Aranhas; Molecular anatomical; Sequencia de DNA; Sequenciamento. |
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Thesaurus NAL: |
Araneae; Sequence analysis; Transcriptomics. |
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Categoria do assunto: |
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G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/56400/1/journal.pone.0021634.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/42811/1/spinningjournal.pone.0021634.pdf
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Marc: |
LEADER 02680naa a2200277 a 4500
001 1920286
005 2018-02-05
008 2011 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pone.0021634$2DOI
100 1 $aPROSDOCIMI, P
245 $aSpinning gland transcriptomics from two main Clades of Spiders (Order$bAraneae) - insights on their molecular, anatomical and behavioral evolution.$h[electronic resource]
260 $c2011
520 $aCharacterized by distinctive evolutionary adaptations, spiders provide a comprehensive system for evolutionary and developmental studies of anatomical organs, including silk and venom production. Here we performed cDNA sequencing using massively parallel sequencers (454 GS-FLX Titanium) to generate ~80,000 reads from the spinning gland of Actinopus spp. (infraorder: Mygalomorphae) and Gasteracantha cancriformis (infraorder: Araneomorphae, Orbiculariae clade). Actinopus spp. retains primitive characteristics on web usage and presents a single undifferentiated spinning gland while the orbiculariae spiders have seven differentiated spinning glands and complex patterns of web usage. MIRA, Celera Assembler and CAP3 software were used to cluster NGS reads for each spider. CAP3 unigenes passed through a pipeline for automatic annotation, classification by biological function, and comparative transcriptomics. Genes related to spider silks were manually curated and analyzed. Although a single spidroin gene family was found in Actinopus spp., a vast repertoire of specialized spider silk proteins was encountered in orbiculariae. Astacin-like metalloproteases (meprin subfamily) were shown to be some of the most sampled unigenes and duplicated gene families in G. cancriformis since its evolutionary split from mygalomorphs. Our results confirm that the evolution of the molecular repertoire of silk proteins was accompanied by the (i) anatomical differentiation of spinning glands and (ii) behavioral complexification in the web usage. Finally, a phylogenetic tree was constructed to cluster most of the known spidroins in gene clades. This is the first large-scale, multi-organism transcriptome for spider spinning glands and a first step into a broad understanding of spider web systems biology and evolution.
650 $aAraneae
650 $aSequence analysis
650 $aTranscriptomics
653 $aAranhas
653 $aMolecular anatomical
653 $aSequencia de DNA
653 $aSequenciamento
700 1 $aBITTENCOURT, D.
700 1 $aSILVA, F. R. da
700 1 $aKIRST, M.
700 1 $aMOTA, P. C.
700 1 $aRECH, E. L.
773 $tPlos One, San Francisco$gv. 6, n. 6, p. 1-15, June 2011.
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Embrapa Amazônia Ocidental (CPAA) |
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