Data/hora: 05/06/2024 09:21:45

Biblioteca(s): | Embrapa Gado de Leite; Embrapa Pecuária Sudeste; Embrapa Unidades Centrais.
Data corrente: | 14/06/2018
Data da última atualização: | 06/05/2019
Autoria: | ZAFALON, L. F.; CUNHA, M. L. R. S.; BRANDAO, H. de M.; MOSQUEIRA, V. C. F.; SANTANA, R. C. M.; BARIONI JUNIOR, W.; MARTINS, K. B.; PILON, L. E.
Afiliação: | LUIZ FRANCISCO ZAFALON, CPPSE; Maria L. R. S. Cunha, Instituto de Biociências/Universidade Estadual Paulista - Unesp; HUMBERTO DE MELLO BRANDAO, CNPGL; Vanessa C. F. Mosqueira, Universidade Federal de Ouro Preto; RAUL COSTA MASCARENHAS SANTANA, CPPSE; WALDOMIRO BARIONI JUNIOR, CPPSE; Katheryne B. Martins, Instituto de Biociências/Universidade Estadual Paulista - Unesp; Lucas E. Pilon, Programa de Pós-Graduação em Medicina Veterinária Preventiva/Unesp.
Título: | Relationship between virulence factor genes in coagulase-negative Staphylococcus spp. and failure of antimicrobial treatment of subclinical mastitis in sheep.
Ano de publicação: | 2018
Fonte/Imprenta: | Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 4, p. 579-585, abril 2018
Idioma: | Inglês
Notas: | Título em português: Relação entre genes de fatores de virulência em Staphylococcus spp. coagulase-negativos e a falha do tratamento antimicrobiano da mastite subclínica ovina.
Conteúdo: | Coagulase-negative Staphylococcus spp. (CNS) are the main microorganisms involved in ovine mastitis. Treatment at the end of lactation can contribute towards cure and prevention of subclinical cases during the subsequent lactation. However, virulence factors and resistance mechanisms presented by CNS can decrease cure rates. The aims of the study were to identify the species of CNS in milk of mastitic ewes with and without antimicrobial treatment, and to investigate the presence of genes relating to resistance of ?-lactam antimicrobials, formation of biofilms, production of enterotoxins and production of the toxic shock syndrome toxin. Cases of failure in the treatment were related with the presence/absence of the respective genes. Sixty sheep were divided into three groups: G1, without treatment; G2, animals treated via the intramammary route with 100mg of cloxacillin during drying off; and G3, sheep treated via the intramammary route with 50 mg of nanoparticulate cloxacillin. Milk samples were gathered during drying off and 15 and 30 days after the parturition of the subsequent lactation. The analyses to identify the species of CNS were carried out by means of the internal transcribe spacer technique and the investigation of the genes responsible for the virulence factors and resistance to oxacillin was performed using the polymerase chain reaction (PCR) technique. No sample was positive for the mecA gene. The only gene relating to production of enterotoxins was sec. Among the genes relating to production of biofilm, icaD was the only one identified in the three experimental groups. Staphylococcus warneri was the main species of CNS isolated during the pre and post-partum periods of the sheep. The species carrying genes relating to production of enterotoxins and biofilms were present in uncured sheep.
Palavras-Chave: | Antimicrobial treatment; Biofilms; Coagulase-negative; Staphylococcus spp; Virulence factor genes.
Thesagro: | Bacteriose; Biofilme; Gene; Ovino.
Thesaurus Nal: | Enterotoxins; Mastitis; Sheep.

Categoria do assunto:

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H Saúde e Patologia

URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/178657/1/Relationship-between-virulence-factor-genes.pdf
Registro original: | Embrapa Pecuária Sudeste (CPPSE)

Exemplares:

AI-SEDE - (62713-1) - UPE - AP - DD -  -  -  - DIS
CPPSE - (24430-1) - UPC - AP - DD - PROCI-2018.00046 - ZAF - 2018.00057 - DIS
CNPGL - (24321-1) - UPC - AP - DD -  -  -  - DIS