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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
27/04/2011 |
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Data da última atualização: |
29/04/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
GOMES, L. M. C.; CASTRO, A. V.; DIAS, L. O.; GOMES, F. P.; ALMEIDA, A-A. F.; PIROVANI, C. P.; GESTEIRA, A. da S. |
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Afiliação: |
Luana Mahé Costa Gomes, UESC; A. V. CASTRO, UESC; L. O. DIAS, UESC; Fábio Pinto Gomes, UESC; Alex-Alan Furtado Almeida, UESC; Carlos Priminho Pirovani, UESC; ABELMON DA SILVA GESTEIRA, CNPMF. |
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Título: |
Cadmium-induced changes in leaf protein profile of Caesalpinia peltophoroides Benth (sibipiruna): protein extraction and bidimentional eletrophoresis. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos... [S. l.]: Sociedade Brasileira de Genética, 2011. 1 CD-ROM. |
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Idioma: |
Português |
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Notas: |
pdf 34634 |
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Conteúdo: |
The specie C.peltophoroides is used as bioindicator of environmental pollution, being suitable for revegetation of degraded areas. Works related to cadmium (Cd) tolerance in this species are still being initiated. In this way, analysis of protein profile aims to assist in the identification of stress-induced protein expression. Seedlings growing in nutrient solution were submitted to two treatments: no Cd and high Cd concentration (32 mg L-1) in a completely randomized design. Leaves from the two treatments were collected at different times of exposure to Cd (0, 6, 12, 24, 48, 72 and 96 h) and fixed in liquid N2. All samples were freeze dried, macerated and washed with TCA/acetone, TCA/water and finally acetone. Proteins were quantified using 2-D Quanti kit (GE Healthcare) and analyzed by SDS-PAGE and 2D-PAGE. Preliminary analysis of proteins in SDS-PAGE showed several bands below 25 kDa, when compared with the control (no Cd), in differents exposure times. The two samples from different exposure times to Cd (24 and 72 h) were selected after analysis of SDS-PAGE and were used in two-dimensional electrophoresis. For isoelectric focusing, we used 380 ?g of protein sample diluted in rehydration buffer strips on linear 13 cm (pH 4-7) using equipment IPGphor 3. The second dimension was performed on polyacrylamide gels with 12.5% in the system Hoefer SE 600 Ruby ?. The gels were fixed with 10% acetic acid and 40% ethanol, stained with Coomassie Brilliant Blue and faded washes in Milli-Q water until the appearance of spots. The gel images were captured in 2D Scanner Magic and analyzed with the software ImageMaster 2D Platinum (Amersham Biosciences) and manual detection. In 2D-PAGE spots were visualized 99 spots (41 differentially expressed) in the 24 h and 122 spots (29 differentially expressed) in the 72 h, both with quality. Most intense spots were analyzed in the Expasy Tagident (www.expasy.ch/), based on its mass and isoelectric point. Taxonomic group and keywords were used to search in the protein database. Preliminary results identified proteins such as: aquaporins, heat shock (HSP), cold and drought-regulated protein (CORA), photosystem I assembly, NAD(P)H-quinone oxidoreductase subunit and cytochrome b6-f complex subunit in Populus, Arabidopsis and Pisum sativum. Some spots were not found in the database. Spots will be selected for the protein sequencing by mass spectrometry. This is a pioneering study involving proteomic data in order to support the hypothesis that C. peltophoroides can be considered a promising species in tolerance to Cd stress. MenosThe specie C.peltophoroides is used as bioindicator of environmental pollution, being suitable for revegetation of degraded areas. Works related to cadmium (Cd) tolerance in this species are still being initiated. In this way, analysis of protein profile aims to assist in the identification of stress-induced protein expression. Seedlings growing in nutrient solution were submitted to two treatments: no Cd and high Cd concentration (32 mg L-1) in a completely randomized design. Leaves from the two treatments were collected at different times of exposure to Cd (0, 6, 12, 24, 48, 72 and 96 h) and fixed in liquid N2. All samples were freeze dried, macerated and washed with TCA/acetone, TCA/water and finally acetone. Proteins were quantified using 2-D Quanti kit (GE Healthcare) and analyzed by SDS-PAGE and 2D-PAGE. Preliminary analysis of proteins in SDS-PAGE showed several bands below 25 kDa, when compared with the control (no Cd), in differents exposure times. The two samples from different exposure times to Cd (24 and 72 h) were selected after analysis of SDS-PAGE and were used in two-dimensional electrophoresis. For isoelectric focusing, we used 380 ?g of protein sample diluted in rehydration buffer strips on linear 13 cm (pH 4-7) using equipment IPGphor 3. The second dimension was performed on polyacrylamide gels with 12.5% in the system Hoefer SE 600 Ruby ?. The gels were fixed with 10% acetic acid and 40% ethanol, stained with Coomassie Brilliant Blue and faded washes in M... Mostrar Tudo |
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Palavras-Chave: |
Environmental pollution; Proteomic. |
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Thesaurus Nal: |
abiotic stress; heavy metals; phytoremediation. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03475nam a2200253 a 4500
001 1886916
005 2011-04-29
008 2011 bl uuuu u00u1 u #d
100 1 $aGOMES, L. M. C.
245 $aCadmium-induced changes in leaf protein profile of Caesalpinia peltophoroides Benth (sibipiruna)$bprotein extraction and bidimentional eletrophoresis.
260 $aIn: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos... [S. l.]: Sociedade Brasileira de Genética, 2011. 1 CD-ROM.$c2011
500 $apdf 34634
520 $aThe specie C.peltophoroides is used as bioindicator of environmental pollution, being suitable for revegetation of degraded areas. Works related to cadmium (Cd) tolerance in this species are still being initiated. In this way, analysis of protein profile aims to assist in the identification of stress-induced protein expression. Seedlings growing in nutrient solution were submitted to two treatments: no Cd and high Cd concentration (32 mg L-1) in a completely randomized design. Leaves from the two treatments were collected at different times of exposure to Cd (0, 6, 12, 24, 48, 72 and 96 h) and fixed in liquid N2. All samples were freeze dried, macerated and washed with TCA/acetone, TCA/water and finally acetone. Proteins were quantified using 2-D Quanti kit (GE Healthcare) and analyzed by SDS-PAGE and 2D-PAGE. Preliminary analysis of proteins in SDS-PAGE showed several bands below 25 kDa, when compared with the control (no Cd), in differents exposure times. The two samples from different exposure times to Cd (24 and 72 h) were selected after analysis of SDS-PAGE and were used in two-dimensional electrophoresis. For isoelectric focusing, we used 380 ?g of protein sample diluted in rehydration buffer strips on linear 13 cm (pH 4-7) using equipment IPGphor 3. The second dimension was performed on polyacrylamide gels with 12.5% in the system Hoefer SE 600 Ruby ?. The gels were fixed with 10% acetic acid and 40% ethanol, stained with Coomassie Brilliant Blue and faded washes in Milli-Q water until the appearance of spots. The gel images were captured in 2D Scanner Magic and analyzed with the software ImageMaster 2D Platinum (Amersham Biosciences) and manual detection. In 2D-PAGE spots were visualized 99 spots (41 differentially expressed) in the 24 h and 122 spots (29 differentially expressed) in the 72 h, both with quality. Most intense spots were analyzed in the Expasy Tagident (www.expasy.ch/), based on its mass and isoelectric point. Taxonomic group and keywords were used to search in the protein database. Preliminary results identified proteins such as: aquaporins, heat shock (HSP), cold and drought-regulated protein (CORA), photosystem I assembly, NAD(P)H-quinone oxidoreductase subunit and cytochrome b6-f complex subunit in Populus, Arabidopsis and Pisum sativum. Some spots were not found in the database. Spots will be selected for the protein sequencing by mass spectrometry. This is a pioneering study involving proteomic data in order to support the hypothesis that C. peltophoroides can be considered a promising species in tolerance to Cd stress.
650 $aabiotic stress
650 $aheavy metals
650 $aphytoremediation
653 $aEnvironmental pollution
653 $aProteomic
700 1 $aCASTRO, A. V.
700 1 $aDIAS, L. O.
700 1 $aGOMES, F. P.
700 1 $aALMEIDA, A-A. F.
700 1 $aPIROVANI, C. P.
700 1 $aGESTEIRA, A. da S.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
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Tipo/Formato |
Classificação |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Solos; Embrapa Unidades Centrais. |
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Data corrente: |
05/08/2002 |
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Data da última atualização: |
14/10/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MOREIRA, G. N. C.; BRITTO, D.P-P. de S.; DYNIA, J. F.; BLOISE, R. M. |
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Afiliação: |
GISA NARA C. MOREIRA, EMBRAPA-SNLCS; DIRCE PINTO PACCA DE SOUZA BRITTO, EMBRAPA; JOSÉ FLÁVIO DYNIA, EMBRAPA-SNLCS; RAPHAEL MINOTTI BLOISE, EMBRAPA-SNLCS. |
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Título: |
Fertilidade de solos da região da rodovia transamazônica. III. Ensaios de adubação. |
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Ano de publicação: |
1977 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 12 (único), p. 81-90, 1977. |
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Idioma: |
Português |
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Notas: |
Título em inglês: Fertility of soils in the region of transamazonic highway. III. Fertilizer experiments. |
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Conteúdo: |
Estudou-se, em casa de vegetação, a resposta de sorgo à aplicação de N, P205, K20 e mistura de Mg, S, Zn, B e Mo (M) em dois solos, Pocizálico Vermelho-Amarelo - PVA - e Terra Roxa Estruturada Latossólica - TRE-L - ocorrentes ao longo da Rodovia Transamazônica. Para o primeiro solo --PVA - constatou-se, nas condições do experimento, a necessidade de aplicação de N, P-205 e K20 em níveis de 207, 251 e 191 kg/ha, respectivamente. Observou-se, ainda, resposta à mistura de M9, S e micronutrientes (Zn, B e Mo). A calagem (2 ton/ha), sem o concurso da adubação, não teve efeito nesse tipo de solo. Na TRE-L, que não apresentava alumínio trocável, houve resposta a nitrogênio, fósforo e interação desses macronutrientes, com efeito linear significativo nos três casos. 0 potássio não influiu no aumento da produção. |
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Palavras-Chave: |
Adubação mineral; Experimento em vaso; resposta à adubação mineral em PVA e TRE-L. |
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Thesagro: |
Fertilidade; Solo Tropical; Sorgo. |
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Categoria do assunto: |
--
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/195031/1/Fertilidade-de-solos-da-regiao-da-rodovia-transamazonica.III.pdf
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Marc: |
LEADER 01673naa a2200241 a 4500
001 2135330
005 2021-10-14
008 1977 bl uuuu u00u1 u #d
100 1 $aMOREIRA, G. N. C.
245 $aFertilidade de solos da região da rodovia transamazônica. III. Ensaios de adubação.
260 $c1977
500 $aTítulo em inglês: Fertility of soils in the region of transamazonic highway. III. Fertilizer experiments.
520 $aEstudou-se, em casa de vegetação, a resposta de sorgo à aplicação de N, P205, K20 e mistura de Mg, S, Zn, B e Mo (M) em dois solos, Pocizálico Vermelho-Amarelo - PVA - e Terra Roxa Estruturada Latossólica - TRE-L - ocorrentes ao longo da Rodovia Transamazônica. Para o primeiro solo --PVA - constatou-se, nas condições do experimento, a necessidade de aplicação de N, P-205 e K20 em níveis de 207, 251 e 191 kg/ha, respectivamente. Observou-se, ainda, resposta à mistura de M9, S e micronutrientes (Zn, B e Mo). A calagem (2 ton/ha), sem o concurso da adubação, não teve efeito nesse tipo de solo. Na TRE-L, que não apresentava alumínio trocável, houve resposta a nitrogênio, fósforo e interação desses macronutrientes, com efeito linear significativo nos três casos. 0 potássio não influiu no aumento da produção.
650 $aFertilidade
650 $aSolo Tropical
650 $aSorgo
653 $aAdubação mineral
653 $aExperimento em vaso
653 $aresposta à adubação mineral em PVA e TRE-L
700 1 $aBRITTO, D.P-P. de S.
700 1 $aDYNIA, J. F.
700 1 $aBLOISE, R. M.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 12 (único), p. 81-90, 1977.
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