|
|
Registros recuperados : 1 | |
1. | | BEVILAQUA, G. A. P.; MIURA, A. K.; SANTOS, A. C.; INHAIA, A. A.; SCHIAVON, M. S. Diagnóstico da sucessão e renda familiar e manejo da agrobiodiversidade na rede de sementes crioulas e agroecológicas do Rio Grande do Sul. Cadernos de Agroecologia, v. 17, n. 3, 2022. Anais da Reunião Técnica sobre Agroecologia: Agroecologia, Resiliência e Bem Viver, 2021, Pelotas. Biblioteca(s): Embrapa Clima Temperado. |
| |
Registros recuperados : 1 | |
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Suínos e Aves. |
Data corrente: |
13/10/2021 |
Data da última atualização: |
13/10/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PETRY, B.; MOREIRA, G. C. M.; COPOLA, A. G. L.; SOUZA, M. M. de; VEIGA, F. C. da; JORGE, E. C.; PEIXOTO, J. de O.; LEDUR, M. C.; KOLTES, J. E.; COUTINHO, L. L. |
Afiliação: |
BRUNA PETRY, Esalq; GABRIEL COSTA MONTEIRO MOREIRA, Esalq; ALINE GONÇALVES LIO COPOLA, UFMG; MARCELA MARIA DE SOUZA, Iowa State University; FERNANDA CRISTINA DA VEIGA, Esalq; ERIKA CRISTINA JORGE, UFMG; JANE DE OLIVEIRA PEIXOTO, CNPSA; MONICA CORREA LEDUR, CNPSA; JAMES E. KOLTES, Iowa State University; LUIZ LEHMANN COUTINHO, Esalq. |
Título: |
SAP30 gene is a probable regulator of muscle hypertrophy in chickens. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Frontiers in Genetic, v. 12, n. 709937, 2021. |
DOI: |
10.3389/fgene.2021.709937 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Animals with muscle hypertrophy phenotype are targeted by the broiler industry to increase the meat production and the quality of the final product. Studies characterizing the molecular machinery involved with these processes, such as quantitative trait loci studies, have been carried out identifying several candidate genes related to this trait; however, validation studies of these candidate genes in cell culture is scarce. The aim of this study was to evaluate SAP30 as a candidate gene for muscle development and to validate its function in cell culture in vitro. The SAP30 gene was downregulated in C2C12 muscle cell culture using siRNA technology to evaluate its impact on morphometric traits and gene expression by RNA-seq analysis. Modulation of SAP30 expression increased C2C12 myotube area, indicating a role in muscle hypertrophy. RNA-seq analysis identified several upregulated genes annotated in muscle development in treated cells (SAP30-knockdown), corroborating the role of SAP30 gene in muscle development regulation. Here, we provide experimental evidence of the involvement of SAP30 gene as a regulator of muscle cell hypertrophy. |
Palavras-Chave: |
C2C12; Crescimento de células musculares; Hipertrofia; Knockdown; Muscle cell growth; Qualidade da carne; SiRNA. |
Thesagro: |
Frango de Corte; Genética Animal; Genética Molecular; Melhoramento Genético Animal; Produção de Carne. |
Thesaurus NAL: |
Hypertrophy; Skeletal muscle. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02313naa a2200409 a 4500 001 2135302 005 2021-10-13 008 2021 bl uuuu u00u1 u #d 024 7 $a10.3389/fgene.2021.709937$2DOI 100 1 $aPETRY, B. 245 $aSAP30 gene is a probable regulator of muscle hypertrophy in chickens.$h[electronic resource] 260 $c2021 520 $aAbstract: Animals with muscle hypertrophy phenotype are targeted by the broiler industry to increase the meat production and the quality of the final product. Studies characterizing the molecular machinery involved with these processes, such as quantitative trait loci studies, have been carried out identifying several candidate genes related to this trait; however, validation studies of these candidate genes in cell culture is scarce. The aim of this study was to evaluate SAP30 as a candidate gene for muscle development and to validate its function in cell culture in vitro. The SAP30 gene was downregulated in C2C12 muscle cell culture using siRNA technology to evaluate its impact on morphometric traits and gene expression by RNA-seq analysis. Modulation of SAP30 expression increased C2C12 myotube area, indicating a role in muscle hypertrophy. RNA-seq analysis identified several upregulated genes annotated in muscle development in treated cells (SAP30-knockdown), corroborating the role of SAP30 gene in muscle development regulation. Here, we provide experimental evidence of the involvement of SAP30 gene as a regulator of muscle cell hypertrophy. 650 $aHypertrophy 650 $aSkeletal muscle 650 $aFrango de Corte 650 $aGenética Animal 650 $aGenética Molecular 650 $aMelhoramento Genético Animal 650 $aProdução de Carne 653 $aC2C12 653 $aCrescimento de células musculares 653 $aHipertrofia 653 $aKnockdown 653 $aMuscle cell growth 653 $aQualidade da carne 653 $aSiRNA 700 1 $aMOREIRA, G. C. M. 700 1 $aCOPOLA, A. G. L. 700 1 $aSOUZA, M. M. de 700 1 $aVEIGA, F. C. da 700 1 $aJORGE, E. C. 700 1 $aPEIXOTO, J. de O. 700 1 $aLEDUR, M. C. 700 1 $aKOLTES, J. E. 700 1 $aCOUTINHO, L. L. 773 $tFrontiers in Genetic$gv. 12, n. 709937, 2021.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Suínos e Aves (CNPSA) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|