| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Unidades Centrais. |
|
Data corrente: |
22/04/2016 |
|
Data da última atualização: |
22/04/2016 |
|
Autoria: |
GRESSLER, L. T.; VARGAS, A. C. de; COSTA, M. M. da; SUTILI, F. J.; SCHWAB, M.; PEREIRA, D. I. B.; SANGIONI, L. A.; BOTTON, S. de A. |
|
Afiliação: |
LETÍCIA T. GRESSLER, UFSM; AGUEDA C. DE VARGAS, UFSM; MATEUS M. DA COSTA, UNIVASF; FERNANDO JONAS SUTILI, UFSM; MARCELO SCHWAB, UFSM; DANIELA ISABEL B. PEREIRA, UFPEL; LUÍS ANTONIO SANGIONI, UFSM; SÔNIA DE A. BOTTON, UFSM. |
|
Título: |
Biofilm formation by Rhodococcus equi and putative association with macrolide resistance. |
|
Ano de publicação: |
2015 |
|
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n. 10, p. 835-841, out. 2015. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Rhodococcus equi is a facultative intracellular pathogen, which cause severe pyogranulomatous pneumonia in foals and tuberculosis-like lesions in humans. Its ability to form biofilm was described in strains isolated from chronic diseases associated to treatment failures in humans. This study aimed to verify the biofilm formation by 113 R. equi isolated from equine samples (clinical and fecal) using two different methods (biofilm--culturing with and without additional glucose and epifluorescence microscopy). We also aimed to determine the efficacy of azithromycin, clarithromycin and erythromycin on R. equi in established biofilm. We found 80.5% (26/41) and 63% (58/72) biofilm--positive isolates, in fecal and clinical samples, respectively. The additional glucose increased the biofilm formation by R. equi fecal samples, but not by clinical samples. The antimicrobials tested herein were not able to eradicate R. equi in biofilm even at higher concentrations. This is the first study showing the biofilm formation by R. equi isolated from equine samples. Our findings indicate that R. equi biofilm-producers may be more resistant to the antimicrobials evaluated. Further studies are warranted to test this hypothesis. |
|
Palavras-Chave: |
DAPI; Macrolídeos; Resistência antimicrobiana. |
|
Thesagro: |
Biofilme. |
|
Thesaurus Nal: |
Antibiotic resistance; Biofilm; Macrolides; Rhodococcus equi. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/142499/1/Biofilm-formation.pdf
|
|
Marc: |
LEADER 02088naa a2200301 a 4500
001 2043798
005 2016-04-22
008 2015 bl uuuu u00u1 u #d
100 1 $aGRESSLER, L. T.
245 $aBiofilm formation by Rhodococcus equi and putative association with macrolide resistance.
260 $c2015
520 $aRhodococcus equi is a facultative intracellular pathogen, which cause severe pyogranulomatous pneumonia in foals and tuberculosis-like lesions in humans. Its ability to form biofilm was described in strains isolated from chronic diseases associated to treatment failures in humans. This study aimed to verify the biofilm formation by 113 R. equi isolated from equine samples (clinical and fecal) using two different methods (biofilm--culturing with and without additional glucose and epifluorescence microscopy). We also aimed to determine the efficacy of azithromycin, clarithromycin and erythromycin on R. equi in established biofilm. We found 80.5% (26/41) and 63% (58/72) biofilm--positive isolates, in fecal and clinical samples, respectively. The additional glucose increased the biofilm formation by R. equi fecal samples, but not by clinical samples. The antimicrobials tested herein were not able to eradicate R. equi in biofilm even at higher concentrations. This is the first study showing the biofilm formation by R. equi isolated from equine samples. Our findings indicate that R. equi biofilm-producers may be more resistant to the antimicrobials evaluated. Further studies are warranted to test this hypothesis.
650 $aAntibiotic resistance
650 $aBiofilm
650 $aMacrolides
650 $aRhodococcus equi
650 $aBiofilme
653 $aDAPI
653 $aMacrolídeos
653 $aResistência antimicrobiana
700 1 $aVARGAS, A. C. de
700 1 $aCOSTA, M. M. da
700 1 $aSUTILI, F. J.
700 1 $aSCHWAB, M.
700 1 $aPEREIRA, D. I. B.
700 1 $aSANGIONI, L. A.
700 1 $aBOTTON, S. de A.
773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 35, n. 10, p. 835-841, out. 2015.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Suínos e Aves. |
|
Data corrente: |
28/11/2019 |
|
Data da última atualização: |
06/12/2019 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 2 |
|
Autoria: |
MOREIRA, G. C. M.; POLETI, M. D.; PÉRTILLE, F.; BOSCHIERO, C.; CESAR, A. S. M.; GODOY, T. F.; LEDUR, M. C.; REECY, J. M.; GARRICK, D. J.; COUTINHO, L. L. |
|
Afiliação: |
GABRIEL COSTA MONTEIRO MOREIRA, USP; USP; USP; CLARISSA BOSCHIERO, USP; ALINE SILVA MELLO CESAR, USP; THAIS FERNANDA GODOY, USP; MONICA CORREA LEDUR, CNPSA; JAMES M. REECY, USP; DORIAN J. GARRICK, USP; LUIZ LEHMANN COUTINHO, USP. |
|
Título: |
Unraveling genomic associations with feed efficiency and body weight traits in chickens through an integrative approach. |
|
Ano de publicação: |
2019 |
|
Fonte/Imprenta: |
BMC Genetics, v. 20, n. 83, 2019. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract Background: Feed efficiency and growth rate have been targets for selection to improve chicken production. The incorporation of genomic tools may help to accelerate selection. We genotyped 529 individuals using a high-density SNP chip (600 K, Affymetrix®) to estimate genomic heritability of performance traits and to identify genomic regions and their positional candidate genes associated with performance traits in a Brazilian F2 Chicken Resource population. Regions exhibiting selection signatures and a SNP dataset from resequencing were integrated with the genomic regions identified using the chip to refine the list of positional candidate genes and identify potential causative mutations. Results: Feed intake (FI), feed conversion ratio (FC), feed efficiency (FE) and weight gain (WG) exhibited low genomic heritability values (i.e. from 0.0002 to 0.13), while body weight at hatch (BW1), 35 days-of-age (BW35), and 41 days-of-age (BW41) exhibited high genomic heritability values (i.e. from 0.60 to 0.73) in this F2 population. Twenty unique 1-Mb genomic windows were associated with BW1, BW35 or BW41, located on GGA1?4, 6?7, 10, 14, 24, 27 and 28. Thirty-eight positional candidate genes were identified within these windows, and three of them overlapped with selection signature regions. Thirteen predicted deleterious and three high impact sequence SNPs in these QTL regions were annotated in 11 positional candidate genes related to osteogenesis, skeletal muscle development, growth, energy metabolism and lipid metabolism, which may be associated with body weight in chickens. Conclusions: The use of a high-density SNP array to identify QTL which were integrated with whole genome sequence signatures of selection allowed the identification of candidate genes and candidate causal variants. One novel QTL was detected providing additional information to understand the genetic architecture of body weight traits. We identified QTL for body weight traits, which were also associated with fatness in the same population. Our findings form a basis for further functional studies to elucidate the role of specific genes in regulating body weight and fat deposition in chickens, generating useful information for poultry breeding programs. MenosAbstract Background: Feed efficiency and growth rate have been targets for selection to improve chicken production. The incorporation of genomic tools may help to accelerate selection. We genotyped 529 individuals using a high-density SNP chip (600 K, Affymetrix®) to estimate genomic heritability of performance traits and to identify genomic regions and their positional candidate genes associated with performance traits in a Brazilian F2 Chicken Resource population. Regions exhibiting selection signatures and a SNP dataset from resequencing were integrated with the genomic regions identified using the chip to refine the list of positional candidate genes and identify potential causative mutations. Results: Feed intake (FI), feed conversion ratio (FC), feed efficiency (FE) and weight gain (WG) exhibited low genomic heritability values (i.e. from 0.0002 to 0.13), while body weight at hatch (BW1), 35 days-of-age (BW35), and 41 days-of-age (BW41) exhibited high genomic heritability values (i.e. from 0.60 to 0.73) in this F2 population. Twenty unique 1-Mb genomic windows were associated with BW1, BW35 or BW41, located on GGA1?4, 6?7, 10, 14, 24, 27 and 28. Thirty-eight positional candidate genes were identified within these windows, and three of them overlapped with selection signature regions. Thirteen predicted deleterious and three high impact sequence SNPs in these QTL regions were annotated in 11 positional candidate genes related to osteogenesis, skeletal muscle development... Mostrar Tudo |
|
Palavras-Chave: |
Características de desempenho; Genomic heritability; Genotypic data; GWAS; Herdabilidade genômica; Performance traits. |
|
Thesagro: |
Frango de Corte; Genoma; Seleção Genótipa. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 03240naa a2200337 a 4500
001 2115438
005 2019-12-06
008 2019 bl uuuu u00u1 u #d
100 1 $aMOREIRA, G. C. M.
245 $aUnraveling genomic associations with feed efficiency and body weight traits in chickens through an integrative approach.$h[electronic resource]
260 $c2019
520 $aAbstract Background: Feed efficiency and growth rate have been targets for selection to improve chicken production. The incorporation of genomic tools may help to accelerate selection. We genotyped 529 individuals using a high-density SNP chip (600 K, Affymetrix®) to estimate genomic heritability of performance traits and to identify genomic regions and their positional candidate genes associated with performance traits in a Brazilian F2 Chicken Resource population. Regions exhibiting selection signatures and a SNP dataset from resequencing were integrated with the genomic regions identified using the chip to refine the list of positional candidate genes and identify potential causative mutations. Results: Feed intake (FI), feed conversion ratio (FC), feed efficiency (FE) and weight gain (WG) exhibited low genomic heritability values (i.e. from 0.0002 to 0.13), while body weight at hatch (BW1), 35 days-of-age (BW35), and 41 days-of-age (BW41) exhibited high genomic heritability values (i.e. from 0.60 to 0.73) in this F2 population. Twenty unique 1-Mb genomic windows were associated with BW1, BW35 or BW41, located on GGA1?4, 6?7, 10, 14, 24, 27 and 28. Thirty-eight positional candidate genes were identified within these windows, and three of them overlapped with selection signature regions. Thirteen predicted deleterious and three high impact sequence SNPs in these QTL regions were annotated in 11 positional candidate genes related to osteogenesis, skeletal muscle development, growth, energy metabolism and lipid metabolism, which may be associated with body weight in chickens. Conclusions: The use of a high-density SNP array to identify QTL which were integrated with whole genome sequence signatures of selection allowed the identification of candidate genes and candidate causal variants. One novel QTL was detected providing additional information to understand the genetic architecture of body weight traits. We identified QTL for body weight traits, which were also associated with fatness in the same population. Our findings form a basis for further functional studies to elucidate the role of specific genes in regulating body weight and fat deposition in chickens, generating useful information for poultry breeding programs.
650 $aFrango de Corte
650 $aGenoma
650 $aSeleção Genótipa
653 $aCaracterísticas de desempenho
653 $aGenomic heritability
653 $aGenotypic data
653 $aGWAS
653 $aHerdabilidade genômica
653 $aPerformance traits
700 1 $aPOLETI, M. D.
700 1 $aPÉRTILLE, F.
700 1 $aBOSCHIERO, C.
700 1 $aCESAR, A. S. M.
700 1 $aGODOY, T. F.
700 1 $aLEDUR, M. C.
700 1 $aREECY, J. M.
700 1 $aGARRICK, D. J.
700 1 $aCOUTINHO, L. L.
773 $tBMC Genetics$gv. 20, n. 83, 2019.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Suínos e Aves (CNPSA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
