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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
21/06/2019 |
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Data da última atualização: |
18/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, W. R. de; PACHECO, T. F.; DUARTE, K. E.; SAMPAIO, B. L.; OLIVEIRA, P. A. de; MARTINS, P. M.; SANTIAGO, T. R.; FORMIGHIERI, E. F.; VINECKY, F.; RIBEIRO, A. P.; DIAS, B. B. A.; KOBAYASHI, A. K.; MITCHELL, R. A. C.; RODRIGUES, D. de S.; MOLINARI, H. B. C. |
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Afiliação: |
Wagner Rodrigo de Souza, Bolsista da Embrapa Agroenergia; THALYTA FRAGA PACHECO, CNPAE; Karoline Estefani Duarte, Bolsista da Embrapa Agroenergia; Bruno Leite Sampaio, Bolsista da Embrapa Agroenergia; PATRICIA ABRAO DE OLIVEIRA MOLINARI, CNPAE; Polyana Kelly Martins, Bolsista da Embrapa Agroenergia; Thaís Ribeiro Santiago, Bolsista da Embrapa Agroenergia; EDUARDO FERNANDES FORMIGHIERI, CNPAE; Felipe Vinecky; Ana Paula Ribeiro; BARBARA ANDRADE DIAS BRITO DA CUNHA, CNPAE; ADILSON KENJI KOBAYASHI, CNPAE; Rowan Andrew Craig Mitchell; DASCIANA DE SOUSA RODRIGUES, CNPAE; HUGO BRUNO CORREA MOLINARI, CNPAE. |
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Título: |
Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibility. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Biotechnology for Biofuels, v. 12, n. 111, p. 1-14, 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Sugarcane (Saccharum spp.) covers vast areas of land (around 25 million ha worldwide), and its processing is already linked into infrastructure for producing bioethanol in many countries. This makes it an ideal candidate for improving composition of its residues (mostly cell walls), making them more suitable for cellulosic ethanol production. In this paper, we report an approach to improving saccharification of sugarcane straw by RNAi silencing of the recently discovered BAHD01 gene responsible for feruloylation of grass cell walls. Results: We identified six BAHD genes in the sugarcane genome (SacBAHDs) and generated five lines with substantially decreased SacBAHD01 expression. To find optimal conditions for determining saccharification of sugarcane straw, we tried multiple combinations of solvent and temperature pretreatment conditions, devising a predictive model for finding their effects on glucose release. Under optimal conditions, demonstrated by Organosolv pretreatment using 30% ethanol for 240 min, transgenic lines showed increases in saccharification efficiency of up to 24%. The three lines with improved saccharification efficiency had lower cell-wall ferulate content but unchanged monosaccharide and lignin compositions. Conclusions: The silencing of SacBAHD01 gene and subsequent decrease of cell-wall ferulate contents indicate a promising novel biotechnological approach for improving the suitability of sugarcane residues for cellulosic ethanol production. In addition, the Organosolv pretreatment of the genetically modified biomass and the optimal conditions for the enzymatic hydrolysis presented here might be incorporated in the sugarcane industry for bioethanol production. Keywords: Sugarcane, MenosBackground: Sugarcane (Saccharum spp.) covers vast areas of land (around 25 million ha worldwide), and its processing is already linked into infrastructure for producing bioethanol in many countries. This makes it an ideal candidate for improving composition of its residues (mostly cell walls), making them more suitable for cellulosic ethanol production. In this paper, we report an approach to improving saccharification of sugarcane straw by RNAi silencing of the recently discovered BAHD01 gene responsible for feruloylation of grass cell walls. Results: We identified six BAHD genes in the sugarcane genome (SacBAHDs) and generated five lines with substantially decreased SacBAHD01 expression. To find optimal conditions for determining saccharification of sugarcane straw, we tried multiple combinations of solvent and temperature pretreatment conditions, devising a predictive model for finding their effects on glucose release. Under optimal conditions, demonstrated by Organosolv pretreatment using 30% ethanol for 240 min, transgenic lines showed increases in saccharification efficiency of up to 24%. The three lines with improved saccharification efficiency had lower cell-wall ferulate content but unchanged monosaccharide and lignin compositions. Conclusions: The silencing of SacBAHD01 gene and subsequent decrease of cell-wall ferulate contents indicate a promising novel biotechnological approach for improving the suitability of sugarcane residues for cellulosic ethanol productio... Mostrar Tudo |
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Palavras-Chave: |
Cell-wall acylation; Lignocellulosic feedstock. |
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Thesaurus Nal: |
Biofuels; Biomass; Sugarcane. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/198722/1/Silencing-of-a-BAHD-acyltransferase.pdf
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Marc: |
LEADER 02739naa a2200349 a 4500
001 2109978
005 2019-11-18
008 2019 bl uuuu u00u1 u #d
100 1 $aSOUZA, W. R. de
245 $aSilencing of a BAHD acyltransferase in sugarcane increases biomass digestibility.$h[electronic resource]
260 $c2019
520 $aBackground: Sugarcane (Saccharum spp.) covers vast areas of land (around 25 million ha worldwide), and its processing is already linked into infrastructure for producing bioethanol in many countries. This makes it an ideal candidate for improving composition of its residues (mostly cell walls), making them more suitable for cellulosic ethanol production. In this paper, we report an approach to improving saccharification of sugarcane straw by RNAi silencing of the recently discovered BAHD01 gene responsible for feruloylation of grass cell walls. Results: We identified six BAHD genes in the sugarcane genome (SacBAHDs) and generated five lines with substantially decreased SacBAHD01 expression. To find optimal conditions for determining saccharification of sugarcane straw, we tried multiple combinations of solvent and temperature pretreatment conditions, devising a predictive model for finding their effects on glucose release. Under optimal conditions, demonstrated by Organosolv pretreatment using 30% ethanol for 240 min, transgenic lines showed increases in saccharification efficiency of up to 24%. The three lines with improved saccharification efficiency had lower cell-wall ferulate content but unchanged monosaccharide and lignin compositions. Conclusions: The silencing of SacBAHD01 gene and subsequent decrease of cell-wall ferulate contents indicate a promising novel biotechnological approach for improving the suitability of sugarcane residues for cellulosic ethanol production. In addition, the Organosolv pretreatment of the genetically modified biomass and the optimal conditions for the enzymatic hydrolysis presented here might be incorporated in the sugarcane industry for bioethanol production. Keywords: Sugarcane,
650 $aBiofuels
650 $aBiomass
650 $aSugarcane
653 $aCell-wall acylation
653 $aLignocellulosic feedstock
700 1 $aPACHECO, T. F.
700 1 $aDUARTE, K. E.
700 1 $aSAMPAIO, B. L.
700 1 $aOLIVEIRA, P. A. de
700 1 $aMARTINS, P. M.
700 1 $aSANTIAGO, T. R.
700 1 $aFORMIGHIERI, E. F.
700 1 $aVINECKY, F.
700 1 $aRIBEIRO, A. P.
700 1 $aDIAS, B. B. A.
700 1 $aKOBAYASHI, A. K.
700 1 $aMITCHELL, R. A. C.
700 1 $aRODRIGUES, D. de S.
700 1 $aMOLINARI, H. B. C.
773 $tBiotechnology for Biofuels$gv. 12, n. 111, p. 1-14, 2019.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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Cutter |
Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
29/09/2011 |
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Data da última atualização: |
29/09/2011 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 3 |
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Autoria: |
ROSSI, P.; RUTZ, F.; LIMA, G. J. M. M. de; NUNES, J. K.; ANCIUTI, M. A.; MORAES, P. V. D. de; SILVA, A. G. C. da; SILVEIRA, M. H. D. da; MAIER, J. C. |
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Afiliação: |
PATRICIA ROSSI, Universy of Kentucky; FERNANDO RUTZ, UFPEL; GUSTAVO JULIO MELLO M DE LIMA, CNPSA; JULIANA KLUG NUNES, UFPEL; MARCO ANTONIO ANCIUTI, UFPEL; PEDRO VALERIO DUTRA DE MORAES, Universy de Kentuky; JOÃO GILBERTO CORRÊA DA SILVA, UFPEL; MARIA HELENA DIAS DA SILVEIRA, UTFPR; JOÃO CARLOS MAIER, UFPEL. |
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Título: |
Efeito do adsorvente a base de glucomamano esterificado no desempenho e caracterização visceral de frangos de corte. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Revista Brasileira de Agrociência, Pelotas, v. 16, n. 1-4, p. 91-100, 2010. |
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Idioma: |
Português |
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Notas: |
Projeto: 11.11.11.111. |
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Palavras-Chave: |
Lesão macroscópica. |
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Thesagro: |
Aflatoxina; Frango de Corte; Ganho de Peso; Nutrição Animal; Ração. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/42595/1/Efeito-do-adsorvente.pdf
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Marc: |
LEADER 00903naa a2200289 a 4500
001 1901707
005 2011-09-29
008 2010 bl uuuu u00u1 u #d
100 1 $aROSSI, P.
245 $aEfeito do adsorvente a base de glucomamano esterificado no desempenho e caracterização visceral de frangos de corte.$h[electronic resource]
260 $c2010
500 $aProjeto: 11.11.11.111.
650 $aAflatoxina
650 $aFrango de Corte
650 $aGanho de Peso
650 $aNutrição Animal
650 $aRação
653 $aLesão macroscópica
700 1 $aRUTZ, F.
700 1 $aLIMA, G. J. M. M. de
700 1 $aNUNES, J. K.
700 1 $aANCIUTI, M. A.
700 1 $aMORAES, P. V. D. de
700 1 $aSILVA, A. G. C. da
700 1 $aSILVEIRA, M. H. D. da
700 1 $aMAIER, J. C.
773 $tRevista Brasileira de Agrociência, Pelotas$gv. 16, n. 1-4, p. 91-100, 2010.
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Embrapa Suínos e Aves (CNPSA) |
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