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Registro Completo
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
26/10/2023 |
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Data da última atualização: |
26/10/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
MORAES, G. S. de; MELLO, J. F. de; BRITO, A. C. de Q.; REIS, A.; MICHEREFF, S. J.; GOMES, A. A. M.; CÂMARA, M. P. S.; VIEIRA, J. C. B; SOUZA-MOTA, C. M. de; MACHADO, A. R. |
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Afiliação: |
GLÍCIA SILVA DE MORAES, UNIVERSIDADE FEDERAL DE PERNAMBUCO; JULIANA FERREIRA DE MELLO, UNIVERSIDADE FEDERAL DE PERNAMBUCO; AMANDA CUPERTINO DE QUEIROZ BRITO, UNIVERSIDADE FEDERAL DE PERNAMBUCO; AILTON REIS, CNPH; SAMI JORGE MICHEREFF, UNIVERSIDADE FEDERAL DE PERNAMBUCO; ANDRÉ ANGELO MEDEIROS GOMES, UNIVERSIDADE FEDERAL DE PERNAMBUCO; MARCOS PAZ SARAIVA CÂMARA, UNIVERSIDADE FEDERAL DE PERNAMBUCO; JANIELE CÁSSIA BARBOSA VIEIRA, UNIVERSIDADE FEDERAL DE PERNAMBUCO; CRISTINA MARIA DE SOUZA-MOTA, UNIVERSIDADE FEDERAL DE PERNAMBUCO; ALEXANDRE REIS MACHADO, UNIVERSIDADE FEDERAL DE PERNAMBUCO. |
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Título: |
Phylogeny of Pseudoperonospora causing downy mildew of Cucurbitaceae in Brazil. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
European Journal of Plant Pathology, v. 167, p. 361-370, 2023. |
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DOI: |
https://doi.org/10.1007/s10658-023-02717-9 |
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Idioma: |
Inglês |
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Conteúdo: |
Downy mildew of cucurbits is a disease caused by the Oomycete Pseudoperonospora cubensis. It can cause considerable losses in cucurbit crops. Phylogenetic studies point to the existence of cryptic species of Pseudoperonospora. The objective of this study was to determine, the phylogeny of Pseudoperonospora species afecting cucurbits in diferent regions of Brazil. For this, leaves with symptoms of downy mildew were collected in areas of cucurbits production in the states of Pernambuco, Minas Gerais, Ceará, and the Distrito Federal. For phylogenetic analyses, DNA extraction was performed, and PCR reactions were carried out using primers DC-6 and LR-0, Cox2-F and Cox2-RC4, OomCox1-levup and OomCox1-levlo, FM79-C1 and FMPHY-10b, for amplifcation of the internal transcribed spacer (ITS) region, the mitochondrial cytochrome c oxidase subunit II (cox2), the mitochondrial cytochrome c oxidase subunit I (cox1) and cox2-cox1 intergenic spacer respectively. The nucleotide sequences were analyzed, edited, and aligned to perform Bayesian Inference analysis. The results of the phylogenetic analyses presented in the cox2-cox1-cox2_1-ITS concatenated tree indicate that in Brazil, the downy mildew of cucurbits is caused by phylogenetically distinct lineages of P. cubensis. |
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Palavras-Chave: |
Oomiceto Pseudoperonospora. |
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Thesagro: |
Cucumis Melo; Cucumis Sativus. |
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Thesaurus NAL: |
Genetic variation; Oomycetes; Peronosporaceae; Pseudoperonospora cubensis; Watermelons. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02290naa a2200337 a 4500
001 2157553
005 2023-10-26
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s10658-023-02717-9$2DOI
100 1 $aMORAES, G. S. de
245 $aPhylogeny of Pseudoperonospora causing downy mildew of Cucurbitaceae in Brazil.$h[electronic resource]
260 $c2023
520 $aDowny mildew of cucurbits is a disease caused by the Oomycete Pseudoperonospora cubensis. It can cause considerable losses in cucurbit crops. Phylogenetic studies point to the existence of cryptic species of Pseudoperonospora. The objective of this study was to determine, the phylogeny of Pseudoperonospora species afecting cucurbits in diferent regions of Brazil. For this, leaves with symptoms of downy mildew were collected in areas of cucurbits production in the states of Pernambuco, Minas Gerais, Ceará, and the Distrito Federal. For phylogenetic analyses, DNA extraction was performed, and PCR reactions were carried out using primers DC-6 and LR-0, Cox2-F and Cox2-RC4, OomCox1-levup and OomCox1-levlo, FM79-C1 and FMPHY-10b, for amplifcation of the internal transcribed spacer (ITS) region, the mitochondrial cytochrome c oxidase subunit II (cox2), the mitochondrial cytochrome c oxidase subunit I (cox1) and cox2-cox1 intergenic spacer respectively. The nucleotide sequences were analyzed, edited, and aligned to perform Bayesian Inference analysis. The results of the phylogenetic analyses presented in the cox2-cox1-cox2_1-ITS concatenated tree indicate that in Brazil, the downy mildew of cucurbits is caused by phylogenetically distinct lineages of P. cubensis.
650 $aGenetic variation
650 $aOomycetes
650 $aPeronosporaceae
650 $aPseudoperonospora cubensis
650 $aWatermelons
650 $aCucumis Melo
650 $aCucumis Sativus
653 $aOomiceto Pseudoperonospora
700 1 $aMELLO, J. F. de
700 1 $aBRITO, A. C. de Q.
700 1 $aREIS, A.
700 1 $aMICHEREFF, S. J.
700 1 $aGOMES, A. A. M.
700 1 $aCÂMARA, M. P. S.
700 1 $aVIEIRA, J. C. B
700 1 $aSOUZA-MOTA, C. M. de
700 1 $aMACHADO, A. R.
773 $tEuropean Journal of Plant Pathology$gv. 167, p. 361-370, 2023.
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