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| Registros recuperados : 138 | |
| 42. |  | MORAES, E. A.; OLIVEIRA, J. E.; MATTOSO, L. H. C.; MARTINS, M. A. Liberação de rodamina-b por nanofibras de poli(épsilon-caprolactona) obtidas pela técnica de fiação por sopro em solução. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 6., 2012, Fortaleza. Anais... São Carlos: Embrapa Instrumentação; Fortaleza: Embrapa Agroindústria Tropical, 2012. p. 333-335. Editores: Maria Alice Martins, MOrsyleide de Freitas Rosa, Men de Sá Moreira de Souza Filho, Nicodemos Moreira dos Santos Junior, Odílio Benedito Garrido de Assis, Caue Ribeiro, Luiz Henrique Capparelli Mattoso.| Biblioteca(s): Embrapa Instrumentação. |
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| 44. | | PEREIRA, J.; VIEIRA, I. F.; MORAES, E. A.; REGO, A. S. Níveis de sulfato de zinco em milho (Zea mays) em solos de campo cerrado. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 8, n. 7, p. 187-191, 1973. (Agronomia). Título em inglês: Levels of zinc sulphate in field corn (Zea mays) grown in campo-cerrados.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 46. | | MORAES, E. A. de; OLIVEIRA, J. E. de; MEDEIROS, E. S. de; MATTOSO, L. H. C. Otimização da morfologia de nanofibras poliméricas produzidas por fiação por sopro em solução. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 2., 2010, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação Agropecuária: Embrapa Pecuária Sudeste, 2010. p. 102. (Embrapa Instrumentação Agropecuária. Documentos, 50).| Biblioteca(s): Embrapa Instrumentação. |
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| 50. | | PEIXOTO, N.; BRAZ, L. T.; BANZATTO, D. A.; MORAES, E. A.; MOREIRA, F. da M. Resposta de feijão-vagem a diferentes níveis de fertilidade. Horticultura Brasileira, Brasília, v. 20, n. 4, p. 593-596, dez. 2002.| Biblioteca(s): Embrapa Hortaliças. |
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| 51. | | OLIVEIRA, J. E.; MORAES, E. A.; MARCONCINI, J. M.; MATTOSO, L. H. C.; GLENN, G. M.; MEDEIROS, E. S. Properties of poly(lactic acid) and poly(ethylene oxide) solvent polymer mixtures and nanofibers made by solution blow spinning. Journal of Applied Polymer Science, New York, v. 129, n. 6, p. 3672-3681, 2013.| Biblioteca(s): Embrapa Instrumentação. |
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| 52. | | SILVA, K. F.; BIANCHIN, I.; CATTO, J. B.; SCZESNY-MORAES, E. A.; HONER, M. R.; PAIVA, F. Identificação das espécies de parasitos gastrintestinais de ovinos e avaliação da resistência a anti-helmínticos no Mato Grosso do Sul. In: JORNADA CIENTÍFICA DA EMBRAPA GADO DE CORTE, 1., 2005, Campo Grande, MS: Embrapa Gado de Corte, 2005. 1 p.| Biblioteca(s): Embrapa Gado de Corte. |
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| 55. | | BRANCIO, P. A.; NASCIMENTO JUNIOR, D. do; REGAZZI, A. J.; MORAES, E. A. de; LEITE, G. G. Avaliacao de pastagem nativa dos cerrados submetida a queima anual. 1. Composicao botanica da dieta de bovinos. Revista Brasileira de Zootecnia, Vicosa, v.26, n.3, p.429-437, 1997.| Biblioteca(s): Embrapa Cerrados. |
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| 56. | | BRANCIO, P. A.; NASCIMENTO JUNIOR, D. do; MORAES, E. A. de; REGAZZI, A. J.; LEITE, G. G. Avaliacao de pastagem nativa dos cerrados submetida a queima anual. 2. Qualidade da dieta de bovinos. Revista Brasileira de Zootecnia, Vicosa, v.26, n.3, p.438-442, 1997.| Biblioteca(s): Embrapa Cerrados. |
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| 57. | | SCZESNY-MORAES, E. A.; BIANCHIN, I; PAIVA, F.; SILVA, K. F.; CATTO, J. B.; HONER, M. R. Isolamento e criopreservação de larvas de TRICHOSTRONGYLUS AXEI (NEMATODA, TRICHOSTRONGYLIDAE). In: JORNADA CIENTÍFICA DA EMBRAPA GADO DE CORTE, 1., 2005, Campo Grande, MS: Embrapa Gado de Corte, 2005. 1 p.| Biblioteca(s): Embrapa Gado de Corte. |
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| 58. | | MORAES, E. A.; CARNEIRO, G. E. de S.; CARNEIRO, J. E. de S.; DEL PELOSO, M. J.; RIOS, G. P. Jalo precoce, Xamego, Rudá e Pérola: novas cultivares de feijão para Goiás e Distrito Federal. In: REUNIÃO NACIONAL DE PESQUISA DE FEIJÃO, 5., 1996, Goiânia. Anais. Goiânia: EMBRAPA-CNPAF, 1996. v. 1. p. 390-392. (EMBRAPA-CNPAF. Documentos, 69).| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 59. | | DEL PELOSO, M. J.; TEIXEIRA, S. M.; MORAES, E. A.; SILVA, C. C. da; CHAGAS, F.; FARIA, M. E. de. A cultura do feijão da "seca" nas regiões do Vale do São Patrício e Rio Vermelho, em Goiás, safra 1987/88. Goiânia: EMGOPA, 1989. 16 p. (EMGOPA. Documentos, 11).| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Meio-Norte. |
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| 60. | | DUTRA, L. G.; DYNIA, J. F.; DEL PELOSO, M. J.; SILVEIRA, P. M. da; TEIXEIRA, S. M.; MORAES, E. A. Cultivo do feijoeiro em várzea do Estado de Goiás. In: REUNIÃO SOBRE FEIJAO IRRIGADO (GO, DF, MG, ES, SP, RJ), 1., 1988, Goiânia. Anais. Goiânia: EMBRAPA-CNPAF, 1990. p. 96-97. (EMBRAPA-CNPAF. Documentos, 27).| Biblioteca(s): Embrapa Arroz e Feijão. |
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| Registros recuperados : 138 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
11/01/2018 |
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Data da última atualização: |
27/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
ASCARI, I. J.; ALVES, N. G.; JASMIN, J.; LIMA, R. R.; QUINTAO, C. C. R.; OBERLENDER, G.; MORAES, E. A.; CAMARGO, L. S. de A. |
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Afiliação: |
I. J. ASCARI, UFLA; N. G. ALVES, UFLA; J. JASMIN, UFRJ; Renato Ribeiro Lima, UFLA; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; G. OBERLENDER, IEF Muzambinho, MG; E. A. MORAES, Universidade Federal do Vale do São Francisco; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Addition of insulin-like growth factor I to the maturation medium of bovine oocytes subjected to heat shock: effects on the production of reactive oxygen species, mitochondrial activity and oocyte competence. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Domestic Animal Endocrinology, v. 60, p. 50-60, 2017. |
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DOI: |
10.1016/j.domaniend.2017.03.003 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract This study was performed to investigate the effects of insulin-like growth factor-I (IGF-I) addition to in vitro maturation (IVM) medium on apoptosis, mitochondrial membrane potential, ROS production, and developmental competence of bovine oocytes subjected to heat shock. Two temperatures (conventional: 24 h at 38.5C, or heat shock: 12 h at 41C followed by 12 h at 38.5C) and 3 IGF-I concentrations (0, 25, and 100 ng/mL) were tested during IVM. The oocytes were then fertilized in vitro, and the presumptive zygotes were cultured until reaching the blastocyst stage. There was no interaction between temperature and IGF-I concentration for any variable evaluated (P > 0.05). The addition of IGF-I did not alter the proportion of nuclear maturation, TUNEL-positive oocytes and caspase-3 activity, or blastocyst proportion on Days 7 and 8 post-fertilization. Furthermore, the total number of cells and the number of cells in the inner cell mass (ICM) in the blastocyst were not altered (P > 0.05). However, IGF-I increased (P < 0.05) the mitochondrial membrane potential and the production of ROS in oocytes and decreased (P < 0.05) the proportion of apoptotic cells in the ICM in blastocysts. Heat shock increased (P < 0.05) the proportion of TUNEL-positive oocytes and ROS production and reduced (P < 0.05) the mitochondrial membrane potential. Moreover, heat shock increased (P < 0.05) the apoptosis proportion in the ICM cells. In conclusion, supplementing IVM mediumwith IGF-I may increase the mitochondrial membrane potential and ROS production in oocytes and decrease apoptosis in the ICM in blastocysts. Heat shock for 12 h compromised oocyte developmental competence and increased apoptosis within the ICM cells of the blastocysts. MenosAbstract This study was performed to investigate the effects of insulin-like growth factor-I (IGF-I) addition to in vitro maturation (IVM) medium on apoptosis, mitochondrial membrane potential, ROS production, and developmental competence of bovine oocytes subjected to heat shock. Two temperatures (conventional: 24 h at 38.5C, or heat shock: 12 h at 41C followed by 12 h at 38.5C) and 3 IGF-I concentrations (0, 25, and 100 ng/mL) were tested during IVM. The oocytes were then fertilized in vitro, and the presumptive zygotes were cultured until reaching the blastocyst stage. There was no interaction between temperature and IGF-I concentration for any variable evaluated (P > 0.05). The addition of IGF-I did not alter the proportion of nuclear maturation, TUNEL-positive oocytes and caspase-3 activity, or blastocyst proportion on Days 7 and 8 post-fertilization. Furthermore, the total number of cells and the number of cells in the inner cell mass (ICM) in the blastocyst were not altered (P > 0.05). However, IGF-I increased (P < 0.05) the mitochondrial membrane potential and the production of ROS in oocytes and decreased (P < 0.05) the proportion of apoptotic cells in the ICM in blastocysts. Heat shock increased (P < 0.05) the proportion of TUNEL-positive oocytes and ROS production and reduced (P < 0.05) the mitochondrial membrane potential. Moreover, heat shock increased (P < 0.05) the apoptosis proportion in the ICM cells. In conclusion, supplementing IVM mediumwith IGF-I may inc... Mostrar Tudo |
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Palavras-Chave: |
Mitochondrial membrane potential; ROS production; Thermoprotection. |
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Thesaurus NAL: |
DNA fragmentation; heat stress. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02716naa a2200277 a 4500
001 2085044
005 2023-01-27
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1016/j.domaniend.2017.03.003$2DOI
100 1 $aASCARI, I. J.
245 $aAddition of insulin-like growth factor I to the maturation medium of bovine oocytes subjected to heat shock$beffects on the production of reactive oxygen species, mitochondrial activity and oocyte competence.$h[electronic resource]
260 $c2017
520 $aAbstract This study was performed to investigate the effects of insulin-like growth factor-I (IGF-I) addition to in vitro maturation (IVM) medium on apoptosis, mitochondrial membrane potential, ROS production, and developmental competence of bovine oocytes subjected to heat shock. Two temperatures (conventional: 24 h at 38.5C, or heat shock: 12 h at 41C followed by 12 h at 38.5C) and 3 IGF-I concentrations (0, 25, and 100 ng/mL) were tested during IVM. The oocytes were then fertilized in vitro, and the presumptive zygotes were cultured until reaching the blastocyst stage. There was no interaction between temperature and IGF-I concentration for any variable evaluated (P > 0.05). The addition of IGF-I did not alter the proportion of nuclear maturation, TUNEL-positive oocytes and caspase-3 activity, or blastocyst proportion on Days 7 and 8 post-fertilization. Furthermore, the total number of cells and the number of cells in the inner cell mass (ICM) in the blastocyst were not altered (P > 0.05). However, IGF-I increased (P < 0.05) the mitochondrial membrane potential and the production of ROS in oocytes and decreased (P < 0.05) the proportion of apoptotic cells in the ICM in blastocysts. Heat shock increased (P < 0.05) the proportion of TUNEL-positive oocytes and ROS production and reduced (P < 0.05) the mitochondrial membrane potential. Moreover, heat shock increased (P < 0.05) the apoptosis proportion in the ICM cells. In conclusion, supplementing IVM mediumwith IGF-I may increase the mitochondrial membrane potential and ROS production in oocytes and decrease apoptosis in the ICM in blastocysts. Heat shock for 12 h compromised oocyte developmental competence and increased apoptosis within the ICM cells of the blastocysts.
650 $aDNA fragmentation
650 $aheat stress
653 $aMitochondrial membrane potential
653 $aROS production
653 $aThermoprotection
700 1 $aALVES, N. G.
700 1 $aJASMIN, J.
700 1 $aLIMA, R. R.
700 1 $aQUINTAO, C. C. R.
700 1 $aOBERLENDER, G.
700 1 $aMORAES, E. A.
700 1 $aCAMARGO, L. S. de A.
773 $tDomestic Animal Endocrinology$gv. 60, p. 50-60, 2017.
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