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| 1. |  | GOBBO, S. P.; BUENO, I. C. da S.; CABRAL FILHO, S. L. S.; GODOY, P. B. de; ABDALLA, A. L. Comparação entre parâmetros de técnicas in vitro e in situ de alimentos destinados para ruminantes. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 40., 2003, Santa Maria, RS. Otimizando a produção animal: anais. Santa Maria: Sociedade Brasileira de Zootecnia, 2003. 3 f. 1 CD ROM.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
28/08/2018 |
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Data da última atualização: |
15/01/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
OKINO, C. H.; MONTASSIER, M. F. de; OLIVEIRA, A. P. de; MONTASSIER, H. J. |
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Afiliação: |
CINTIA HIROMI OKINO, CPPSE; Maria de Fátima Silva Montassier, UNESP; Andressa Peres de Oliveira, UNESP; Helio José Montassier, UNESP. |
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Título: |
Rapid detection and differentiation of avian infectious bronchitis virus: an application of Mass genotype by melting temperature analysis in RT-qPCR using SYBR Green I. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Veterinary Medical Science, v. 80, p. 725-730, fev. 2018. |
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Volume: |
80 |
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Idioma: |
Inglês |
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Conteúdo: |
A method based on Melting Temperature analysis of Hypervariable regions (HVR) of S1 gene within a RT-qPCR was developed to detect different genotypes of avian infectious bronchitis virus (IBV) and identify the Mass genotype. The method was able to rapidly identify the Mass genotype among IBV field isolates, vaccine attenuated strains and reference M41 strain in allantoic liquid and also directly in tissues. The RT-qPCR developed detected the virus in both tracheal and pulmonary samples from M41-infected or H120-infected birds, in a larger post-infection period compared to detection by standard method of virus isolation. RT-qPCR method tested provided a sensitivity and rapid approach for screening on IBV detection and Mass genotyping from IBV isolates. |
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Thesagro: |
Bronquite. |
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Thesaurus NAL: |
Birds; Bronchitis; Infectious bronchitis; Infectious bronchitis virus. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/181999/1/OKINO-2018.pdf
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Marc: |
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008 2018 bl uuuu u00u1 u #d
100 1 $aOKINO, C. H.
245 $aRapid detection and differentiation of avian infectious bronchitis virus$ban application of Mass genotype by melting temperature analysis in RT-qPCR using SYBR Green I.$h[electronic resource]
260 $c2018
300 $a80
490 $v80
520 $aA method based on Melting Temperature analysis of Hypervariable regions (HVR) of S1 gene within a RT-qPCR was developed to detect different genotypes of avian infectious bronchitis virus (IBV) and identify the Mass genotype. The method was able to rapidly identify the Mass genotype among IBV field isolates, vaccine attenuated strains and reference M41 strain in allantoic liquid and also directly in tissues. The RT-qPCR developed detected the virus in both tracheal and pulmonary samples from M41-infected or H120-infected birds, in a larger post-infection period compared to detection by standard method of virus isolation. RT-qPCR method tested provided a sensitivity and rapid approach for screening on IBV detection and Mass genotyping from IBV isolates.
650 $aBirds
650 $aBronchitis
650 $aInfectious bronchitis
650 $aInfectious bronchitis virus
650 $aBronquite
700 1 $aMONTASSIER, M. F. de
700 1 $aOLIVEIRA, A. P. de
700 1 $aMONTASSIER, H. J.
773 $tVeterinary Medical Science$gv. 80, p. 725-730, fev. 2018.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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