Data/hora: 29/05/2024 06:20:22

Biblioteca(s): 	Embrapa Pecuária Sudeste.	
Tipo da produção científica: 	Artigo em Periódico Indexado	
Circulação/Nível: 	A - 1	
Data corrente: 	28/08/2018	
Data da última atualização: 	03/01/2019	
Autoria: 	LOPES, P. D.; OKINO, C. H.; FERNANDO, F. S.; PAVANI, C.; CASAGRANDE, V. M.; LOPEZ, R. F. V.; MONTASSIER, M. F. de; MONTASSIER, H. J.	
Afiliação: 	Priscila Diniz Lopes, UNESP; CINTIA HIROMI OKINO, CPPSE; Filipe Santos Fernando, UNESP; Caren Pavani, UNESP; Viviane Mariguela Casagrande, UNESP; Renata F. V. Lopez, USP; Maria de Fátima Silva Montassier, UNESP; Helio José Montassier, UNESP.	
Título: 	Inactivated infectious bronchitis virus vaccine encapsulated in chitosan nanoparticles induces mucosal immune responses and effective protection against challenge.	
Ano de publicação: 	2018	
Fonte/Imprenta: 	Vaccine, v. 36, p. 2630-2636, abr. 2018.	
Volume: 	36	
Idioma: 	Inglês	
Conteúdo: 	Avian infectious bronchitis virus (IBV) is one of the most important viral diseases of poultry. The mucosa of upper respiratory tract, specially the trachea, is the primary replication site for this virus. However, conventional inactivate IBV vaccines usually elicit reduced mucosal immune responses and local protection. Thus, an inactivated IBV vaccine containing BR-I genotype strain encapsulated in chitosan nanoparticles (IBV-CS) was produced by ionic gelation method to be administered by oculo-nasal route to chickens. IBV-CS vaccine administered alone resulted in markedly mucosal immune responses, characterized by high levels of anti-IBV IgA isotype antibodies and IFNc gene expression at 1dpi. The association of live attenuated Massachusetts IBV and IBV-CS vaccine also induced strong mucosal immune responses, though a switch from IgA isotype to IgG was observed, and IFNc gene expression peak was late (at 5 dpi). Efficacy of IBV-CS was evaluated by tracheal ciliostasis analysis, histopathology examination, and viral load determination in the trachea and kidney. The results indicated that IBV-CS vaccine administered alone or associated with a live attenuated heterologous vaccine induced both humoral and cellmediated immune responses at the primary site of viral replication, and provided an effective protection against IBV infection at local (trachea) and systemic (kidney) sites.	
Thesagro: 	Bronquite; Vacina; Vacina Inativada.	
Thesaurus Nal: 	Inactivated vaccines; Infectious bronchitis; Infectious bronchitis virus; Mucosal immunity.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Pecuária Sudeste (CPPSE)	
Exemplares: 	CPPSE - (24422-1) - UPC - AP - DD - PROCI-2018.00041 - LOP - 2018.00053 - DIS