Data/hora: 01/06/2024 17:24:21

Biblioteca(s): | Embrapa Agroenergia.
Tipo da produção científica: | Artigo em Anais de Congresso
Data corrente: | 23/09/2019
Data da última atualização: | 23/09/2019
Autoria: | GREGORINE, L. F.; MONCLARO, A. V.; SILVA, C. de O. G.; RODRIGUES, K. B.; PEIXOTO, J. S. G.; ABDELNUR, P. V.; FAVARO, L. C. de L.
Afiliação: | Lucas F. Gregorine; Antonielle V. Monclaro; Caio de Oliveira Gorgulho Silva, Consultor da Embrapa Agroenergia; Kelly B. Rodrigues; Jéssica S. G. Peixoto, UnB; PATRICIA VERARDI ABDELNUR, CNPAE; LEIA CECILIA DE LIMA FAVARO, CNPAE.
Título: | Heterologous expression of a new lytic polysaccharide monooxygenase from Hahella ganghwensis and their functional characterization.
Ano de publicação: | 2019
Fonte/Imprenta: | In: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 22.; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, 13., 2019, Uberlandia, MG. [Anais ...]. São Paulo: Associação Brasileira de Engenharia Química, 2019.
Idioma: | Inglês
Conteúdo: | The powerful class of oxidative enzymes, lytic polysaccharide monooxygenases (LPMOs) - also named Auxiliary Activity (AA) - are able to oxidize recalcitrant polysaccharides on lignocellulosic biomass. In this work, we successfully expressed three catalytic domains from bacterial LPMOs in the yeast Komagataella phaffii: domain MdAA10.1-SD (from Moritella dasanensis), domain VmAA10.2-SD (from Verrucosispora maris), and domain HgAA10.1-SD (from Hahella ganghwensis). Heterologous expression was analyzed by SDS-PAGE, Western-Blot, and Dot-Blot, while functional activity of these proteins was investigated by a combination of mass spectrometric and chromatographic methods. The recombinant LPMO catalytic domain HgAA10.1-SD from H. ganghwensis, a C1-oxidizer, was able to promote an oxidative cleavage of phosphoric-acid swollen cellulose (PASC) substrate in the presence of ascorbic acid as an electron donor, showing its potential for cellulose depolymerization.
Palavras-Chave: | Chromatographic; Spectrometric.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/202240/1/107193-786998-field-submission-fulltext-file1.pdf
Registro original: | Embrapa Agroenergia (CNPAE)
Exemplares: | CNPAE - (3494-1) - UPC - AA - DD -  -  -  - DIS