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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
04/10/2016 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
POPPL, A. G.; VALLE, S. C.; GONZÁLEZ, F. H. D.; KUCHARSKI, L. C.; SILVA, R. S. M. da. |
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Afiliação: |
ÁLAN G. POPPL, UFRGS; SANDRA C. VALLE, UFRGS; FÉLIX H. D. GONZÁLEZ, UFRGS; LUIZ C. KUCHARSKI, UFRGS; ROSELIS S. M. DA SILVA, UFRGS. |
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Título: |
Insulin binding characteristics in canine muscle tissue: effects of the estrous cycle phases. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 8, p. 761-766, ago. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Hormonal fluctuations during the different estrous cycle are a well-recognized cause of insulin resistance in bitches, and little is known about insulin receptor binding or post-binding defects associated with insulin resistance in dogs. To evaluate insulin binding characteristics in muscle tissue of bitches during the estrous cycle, 17 owned bitches were used in the study (six in anestrus, five in estrus, and six in diestrus). An intravenous glucose tolerance test (IVGTT) was performed in all patients by means of injection of 1mL/kg of a glucose 50% solution (500mg/kg), with blood sample collection for glucose determination at 0, 3, 5, 7, 15, 30, 45 and 60 minutes after glucose infusion. Muscle samples, taken after spaying surgery, were immediately frozen in liquid nitrogen and then stored at -80 ºC until the membranes were prepared by sequential centrifugation after being homogenized. For binding studies, membranes were incubated in the presence of 20,000cpm of human 125I-insulin and in increasing concentrations of unlabeled human regular insulin for cold saturation. The IVGTT showed no differences among bitches during the estrous cycle regarding baseline glycemia or glycemic response after glucose infusion. Two insulin binding sites - high-affinity and low-affinity ones - were detected by Scatchard analysis, and significant statistical differences were observed in the dissociation constant (Kd1) and maximum binding capacity (Bmax1) of the high-affinity binding sites. The Kd1 for the anestrus group (6.54±2.77nM/mg of protein) was smaller (P<0.001) than for the estrus (28.54±6.94nM/mg of protein) and diestrus (15.56±3.88nM/mg of protein) groups. Bmax1 in the estrus (0.83±0.42nM/mg of protein) and diestrus (1.24±0.24nM/mg of protein) groups were also higher (P<0.001) than the values observed in anestrus (0.35±0.06nM/mg of protein). These results indicate modulation of insulin binding characteristics during different phases of the estrous cycle in dogs, showing that muscle insulin binding affinity for its receptor is reduced during estrus and diestrus. However, this poor hormone-receptor affinity is compensated for by a greater total binding capacity, once there is no difference in patients? glycemic response after an intravenous glucose load. MenosHormonal fluctuations during the different estrous cycle are a well-recognized cause of insulin resistance in bitches, and little is known about insulin receptor binding or post-binding defects associated with insulin resistance in dogs. To evaluate insulin binding characteristics in muscle tissue of bitches during the estrous cycle, 17 owned bitches were used in the study (six in anestrus, five in estrus, and six in diestrus). An intravenous glucose tolerance test (IVGTT) was performed in all patients by means of injection of 1mL/kg of a glucose 50% solution (500mg/kg), with blood sample collection for glucose determination at 0, 3, 5, 7, 15, 30, 45 and 60 minutes after glucose infusion. Muscle samples, taken after spaying surgery, were immediately frozen in liquid nitrogen and then stored at -80 ºC until the membranes were prepared by sequential centrifugation after being homogenized. For binding studies, membranes were incubated in the presence of 20,000cpm of human 125I-insulin and in increasing concentrations of unlabeled human regular insulin for cold saturation. The IVGTT showed no differences among bitches during the estrous cycle regarding baseline glycemia or glycemic response after glucose infusion. Two insulin binding sites - high-affinity and low-affinity ones - were detected by Scatchard analysis, and significant statistical differences were observed in the dissociation constant (Kd1) and maximum binding capacity (Bmax1) of the high-affinity binding sites. The ... Mostrar Tudo |
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Palavras-Chave: |
Insulin sensitivity; Receptor tirosina quinase; Resistência insulinica; Tyrosine kinase receptors. |
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Thesagro: |
Cão; Ciclo estral; Musculo. |
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Thesaurus Nal: |
diabetes mellitus; Dogs; Estrous cycle; Insulin resistance; Muscle tissues. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/148294/1/Insulin-binding-characteristics.pdf
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Marc: |
LEADER 03212naa a2200313 a 4500
001 2054054
005 2017-12-29
008 2016 bl uuuu u00u1 u #d
100 1 $aPOPPL, A. G.
245 $aInsulin binding characteristics in canine muscle tissue$beffects of the estrous cycle phases.
260 $c2016
520 $aHormonal fluctuations during the different estrous cycle are a well-recognized cause of insulin resistance in bitches, and little is known about insulin receptor binding or post-binding defects associated with insulin resistance in dogs. To evaluate insulin binding characteristics in muscle tissue of bitches during the estrous cycle, 17 owned bitches were used in the study (six in anestrus, five in estrus, and six in diestrus). An intravenous glucose tolerance test (IVGTT) was performed in all patients by means of injection of 1mL/kg of a glucose 50% solution (500mg/kg), with blood sample collection for glucose determination at 0, 3, 5, 7, 15, 30, 45 and 60 minutes after glucose infusion. Muscle samples, taken after spaying surgery, were immediately frozen in liquid nitrogen and then stored at -80 ºC until the membranes were prepared by sequential centrifugation after being homogenized. For binding studies, membranes were incubated in the presence of 20,000cpm of human 125I-insulin and in increasing concentrations of unlabeled human regular insulin for cold saturation. The IVGTT showed no differences among bitches during the estrous cycle regarding baseline glycemia or glycemic response after glucose infusion. Two insulin binding sites - high-affinity and low-affinity ones - were detected by Scatchard analysis, and significant statistical differences were observed in the dissociation constant (Kd1) and maximum binding capacity (Bmax1) of the high-affinity binding sites. The Kd1 for the anestrus group (6.54±2.77nM/mg of protein) was smaller (P<0.001) than for the estrus (28.54±6.94nM/mg of protein) and diestrus (15.56±3.88nM/mg of protein) groups. Bmax1 in the estrus (0.83±0.42nM/mg of protein) and diestrus (1.24±0.24nM/mg of protein) groups were also higher (P<0.001) than the values observed in anestrus (0.35±0.06nM/mg of protein). These results indicate modulation of insulin binding characteristics during different phases of the estrous cycle in dogs, showing that muscle insulin binding affinity for its receptor is reduced during estrus and diestrus. However, this poor hormone-receptor affinity is compensated for by a greater total binding capacity, once there is no difference in patients? glycemic response after an intravenous glucose load.
650 $adiabetes mellitus
650 $aDogs
650 $aEstrous cycle
650 $aInsulin resistance
650 $aMuscle tissues
650 $aCão
650 $aCiclo estral
650 $aMusculo
653 $aInsulin sensitivity
653 $aReceptor tirosina quinase
653 $aResistência insulinica
653 $aTyrosine kinase receptors
700 1 $aVALLE, S. C.
700 1 $aGONZÁLEZ, F. H. D.
700 1 $aKUCHARSKI, L. C.
700 1 $aSILVA, R. S. M. da
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 8, p. 761-766, ago. 2016.
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Embrapa Unidades Centrais (AI-SEDE) |
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| 4. |  | MIRANDA, A. R. Manejo da colecao de germoplasma-semente. In: PUIGNAU, J.P., ed. Conservacion de germoplasma vegetal. Montevideo: IICA, 1996. p.85-92. (IICA-PROCISUR. Dialogo, 45). p.85-92 (IICA-PROCISUR. Dialogo, 45)| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 17. | | PEREIRA, G. A.; MIRANDA, A. R.; PADILHA, L. S.; MUNDIM, R. C.; ALVES, R. B. N. Conservação Ex situ de variedades tradicionais de milho indígena. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 5., 2000, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2000. p. 60.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 18. | | CÂNDIDO, L. D. W.; DURIGAN, M. F. B.; MIRANDA, A.; COSTA, E.; TEIXEIRA, R. Qualidade de castanhas-do-brasil Bertholletia excelsa H. (Lecythidaceae) cultivadas em três diferentes áreas de Roraima. In: SEMANA NACIONAL DE CIÊNCIA E TECNOLOGIA NO ESTADO DE RORAIMA, SNCT-RR, 9., 2014. Resumos... Boa Vista, RR: UERR, 2014. p. 138| Tipo: Resumo em Anais de Congresso |
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| 19. | | BARILLI, D. R.; PIETROWSKI, V.; WENGRAT, A. P. da S.; MIRANDA, A. M.; RINGENBERG, R. Espécies de moscas-branca associadas à cultura da mandioca (Manihot esculenta Crantz) e parasitoides de Bemisia tuberculata (Bondar, 1923) nos Estados do Mato Grosso do Sul e Paraná. In: CONGRESSO BRASILEIRO DE MANDIOCA, 15., 2013, Salvador. Inovação e sustentabilidade: da raiz ao amido: trabalhos apresentados. Salvador: CBM: Embrapa, 2013. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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