|
|
Registros recuperados : 34 | |
3. | | ROCHA, N. A. S.; LEÃO, B. C. S.; NOGUEIRA, E.; MINGOTI, G. Z. Bovine embryos cultured in vitro in the presence of antioxidants: implications for blastocysts development, quality and cryotolerance. In: INTERNATIONAL SYMPOSIUM ON ANIMAL BIOLOGY OF REPRODUCTION, 4. Animal Reproduction, v.9, n.4, p.829, 2012. (ISABR 2012). Biblioteca(s): Embrapa Pantanal. |
| |
4. | | ROCHA, N. A. S.; LEÃO, B. C. S.; ACCORSI, M. F.; NOGUEIRA, E.; MINGOTI, G. Z. Levels of reactive oxygen species (Ros), apoptosis and cryoresistance of bovine embryos culture in the presence of antioxidants. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 26., 2012, Foz do Iguaçu. Anais. Belo Horizonte: CBRA, 2012. Biblioteca(s): Embrapa Pantanal. |
| |
5. | | ROCHA, N. A. S.; LEÃO, B. C. S.; NOGUEIRA, E.; MINGOTI, G. Z. Supplementation with antioxidants during maturation increases resistance to oxidative stress in bovine embryos pruduced in vitro but does not improve cryotolerance. Animal Reproduction, v. 9. n. 3. p. 681, jul/set, 2012. Publicado no: Proceedings of the 26th Annual Meeting of the Brazilian Embryo Technology Society (SBTE), August 30th to September 2nd, 2012, Foz do Iguaçu, PR, Brazil. Biblioteca(s): Embrapa Pantanal. |
| |
8. | | ROCHA, N. A. de S.; LEÃO, B. C. da S.; ACCORSI, M. F.; NOGUEIRA, E.; MINGOTI, G. Z. Bovine embryo culture in the presence of antioxidants: implications for blastocysts development, cryotolerance and levels of intracellular reactive oxygen species (ROS). Reproduction in Domestic Animals, v. 47, n. 4, p. 531, 2012. Publicado no: Proceedings of the 17th International Congress on Animal Reproduction (ICAR) Vancouver, Canada; 29 July - 2 August 2012. Biblioteca(s): Embrapa Pantanal. |
| |
10. | | MENEGHEL, M.; DALL'ACQUA, P. C.; AMBROGI, M.; LEÃO, B. C. S.; ROCHA-FRIGONI, N. A. S.; MINGOTI, G. Z. Lipid content and cryotolerance of in vitro-produced bovine embryos treated with forskolin before vitrification. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 4, p. 395-400, abril 2017. Título em português: Acúmulo de lipídios intracitoplasmáticos, desenvolvimento e criotolerância de embriões bovinos produzidos in vitro e tratados com diferentes concentrações de forskolin antes da vitrificação. Biblioteca(s): Embrapa Unidades Centrais. |
| |
14. | | ROCHA, N. A. de S.; LEÃO, B. C. da S.; ACCORSI, M. F.; NOGUEIRA, E.; MINGOTI, G. Z. Cultivo in vitro de embriões bovinos na presença de antioxidantes: implicações sobre o desenvolvimento embrionário, criotolerância e níveis intracelulares de espécies reativas de oxigênio. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 26., 2012, Foz do Iguaçu. Anais. Belo Horizonte: CBRA, 2012. Biblioteca(s): Embrapa Pantanal. |
| |
15. | | NOGUEIRA, E.; SILVA, A. S. da; AMARAL, T. B.; ÍTAVO, L. C. V.; DIAS, A. M.; MINGOTI, G. Z. Follicular dynamics and production of oocytes in young Nellore heifers with energetic supplementation. Revista Brasileira de Zootecnia, v. 41, n. 9, p. 2012-2017, 2012. Biblioteca(s): Embrapa Pantanal. |
| |
19. | | MINGOTI, G. Z.; JIMENEZ FILHO, D. L.; FERRAUDO, A.; LEÃO, B. C. S.; ROCHA-FRIGONI, N. A. S.; NOGUEIRA, E.; DUTRA, I. S. Analysis of perception of risk factors for public health in the use of hormones in assisted reproduction programs in cattle. Animal Reproduction, v. 13, n. 3, p. 444, Jul./Sept. 2016. Proceedings of the 30th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Foz do Iguaçu, PR, Brazil, August 25th to 27th, 2016, and 32nd Meeting of the European Embryo Transfer Association (AETE); Barcelona, Spain,... Biblioteca(s): Embrapa Pantanal. |
| |
20. | | LEÃO, B. C. S.; NOGUEIRA, E.; ROCHA, N. A. S.; ACCORSI, M. F.; NEVES, T. V.; MARCOS, M. T. S.; CUNHA JUNIOR, G. F.; MINGOTI, G. Z. Nutritional supplementation of nelore heifers with polyunsaturated fatty acids do not interfere in the development and cryotolerance of in vitro produced embryos. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 26., 2012, Foz do Iguaçu. Anais. Belo Horizonte: CBRA, 2012. Biblioteca(s): Embrapa Pantanal. |
| |
Registros recuperados : 34 | |
|
|
Registro Completo
Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
03/08/2017 |
Data da última atualização: |
29/12/2017 |
Autoria: |
MENEGHEL, M.; DALL'ACQUA, P. C.; AMBROGI, M.; LEÃO, B. C. S.; ROCHA-FRIGONI, N. A. S.; MINGOTI, G. Z. |
Afiliação: |
Melissa Meneghel, Universidade Estadual Paulista-UNESP/Programa de Pós-Graduação em Medicina Veterinária (Reprodução Animal)/Faculdade de Ciências Agrárias e Veterinárias; Priscila Chediek Dall’Acqua, Universidade Estadual Paulista-UNESP/Programa de Pós-Graduação em Medicina Veterinária (Reprodução Animal)/Faculdade de Ciências Agrárias e Veterinárias; Marcela Ambrogi, Universidade Estadual Paulista-UNESP/Programa de Pós-Graduação em Medicina Veterinária (Reprodução Animal)/Faculdade de Ciências Agrárias e Veterinárias; Beatriz C. S. Leão, Universidade Estadual Paulista-UNESP/Programa de Pós-Graduação em Medicina Veterinária (Reprodução Animal)/Faculdade de Ciências Agrárias e Veterinárias; Nathália A. S. Rocha-Frigoni, Universidade Estadual Paulista-UNESP/Programa de Pós-Graduação em Medicina Veterinária (Reprodução Animal)/Faculdade de Ciências Agrárias e Veterinárias; Gisele Z. Mingoti, Universidade Estadual Paulista-UNESP/Programa de Pós-Graduação em Medicina Veterinária (Reprodução Animal)/Faculdade de Ciências Agrárias e Veterinárias. |
Título: |
Lipid content and cryotolerance of in vitro-produced bovine embryos treated with forskolin before vitrification. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 4, p. 395-400, abril 2017. |
Idioma: |
Inglês |
Notas: |
Título em português: Acúmulo de lipídios intracitoplasmáticos, desenvolvimento e criotolerância de embriões bovinos produzidos in vitro e tratados com diferentes concentrações de forskolin antes da vitrificação. |
Conteúdo: |
The aim of the present study was to evaluate the intracytoplasmic lipid content, development and cryotolerance of in vitro-produced bovine embryos treated with different concentrations of forskolin before vitrification. Embryos were produced from abattoir-derived ovaries and allocated into four groups. In the treatment groups, forskolin was added to the in vitro culture medium on Day 6 and incubated for 24 hours in one of the following concentrations: 2.5μM (Forsk 2.5 group), 5.0μM (Forsk 5.0 group) or 10.0μM (Forsk 10.0 group). Embryos from the control group were cultured without forskolin. On Day 7 of culture, the expanded blastocysts were stained with the lipophilic dye Sudan Black B for determination of the intracytoplasmic lipid content or were cryopreserved via the Vitri- -Ingá® procedure. Although there were no significant differences (P>0.05) in the blastocyst rates between the Control group (44.9%) and the other treatments, the embryo production was lower (P<0.05) in Forsk 10.0 group (38.8%) compared to Forsk 2.5 (50.5%) and Forsk 5.0 (54.7%) groups. The intracytoplasmic lipid content (expressed in arbitrary units of pixels) in blastocysts from the Control group (1.00±0.03) was similar (P>0.05) to that found in Forsk 2.5 (0.92±0.03) and Forsk 10.0 groups (1.06±0.03) groups; however the lipid accumulation in blastocysts from Forsk 5.0 group (0.82±0.04) was lower than in the Control group (P<0.05). Based on these results, Forsk 5.0 treatment was tested for cryotolerance and it was observed that the blastocoel re-expansion rate evaluated 24 hours after warming was greater (P<0.05) in Forsk 5.0 group (72.2%) compared to the Control group (46.2%). In conclusion, pre-treatment with forskolin at a concentration of 5.0 μM for 24 hours before vitrification is effective in reducing the intracytoplasmic lipid content and, consequently, improves cryotolerance of IVP bovine embryos. MenosThe aim of the present study was to evaluate the intracytoplasmic lipid content, development and cryotolerance of in vitro-produced bovine embryos treated with different concentrations of forskolin before vitrification. Embryos were produced from abattoir-derived ovaries and allocated into four groups. In the treatment groups, forskolin was added to the in vitro culture medium on Day 6 and incubated for 24 hours in one of the following concentrations: 2.5μM (Forsk 2.5 group), 5.0μM (Forsk 5.0 group) or 10.0μM (Forsk 10.0 group). Embryos from the control group were cultured without forskolin. On Day 7 of culture, the expanded blastocysts were stained with the lipophilic dye Sudan Black B for determination of the intracytoplasmic lipid content or were cryopreserved via the Vitri- -Ingá® procedure. Although there were no significant differences (P>0.05) in the blastocyst rates between the Control group (44.9%) and the other treatments, the embryo production was lower (P<0.05) in Forsk 10.0 group (38.8%) compared to Forsk 2.5 (50.5%) and Forsk 5.0 (54.7%) groups. The intracytoplasmic lipid content (expressed in arbitrary units of pixels) in blastocysts from the Control group (1.00±0.03) was similar (P>0.05) to that found in Forsk 2.5 (0.92±0.03) and Forsk 10.0 groups (1.06±0.03) groups; however the lipid accumulation in blastocysts from Forsk 5.0 group (0.82±0.04) was lower than in the Control group (P<0.05). Based on these results, Forsk 5.0 treatment was tested ... Mostrar Tudo |
Palavras-Chave: |
Acúmulo lipídico intracitoplasmático; Criotolerância; Lipídio intracitoplasmático; Produção in vitro; Vitrificação. |
Thesagro: |
Bovino; Embrião. |
Thesaurus NAL: |
forskolin. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/162413/1/Lipid-content-and-cryotolerance.pdf
|
Marc: |
LEADER 03070naa a2200289 a 4500 001 2073597 005 2017-12-29 008 2017 bl uuuu u00u1 u #d 100 1 $aMENEGHEL, M. 245 $aLipid content and cryotolerance of in vitro-produced bovine embryos treated with forskolin before vitrification.$h[electronic resource] 260 $c2017 500 $aTítulo em português: Acúmulo de lipídios intracitoplasmáticos, desenvolvimento e criotolerância de embriões bovinos produzidos in vitro e tratados com diferentes concentrações de forskolin antes da vitrificação. 520 $aThe aim of the present study was to evaluate the intracytoplasmic lipid content, development and cryotolerance of in vitro-produced bovine embryos treated with different concentrations of forskolin before vitrification. Embryos were produced from abattoir-derived ovaries and allocated into four groups. In the treatment groups, forskolin was added to the in vitro culture medium on Day 6 and incubated for 24 hours in one of the following concentrations: 2.5μM (Forsk 2.5 group), 5.0μM (Forsk 5.0 group) or 10.0μM (Forsk 10.0 group). Embryos from the control group were cultured without forskolin. On Day 7 of culture, the expanded blastocysts were stained with the lipophilic dye Sudan Black B for determination of the intracytoplasmic lipid content or were cryopreserved via the Vitri- -Ingá® procedure. Although there were no significant differences (P>0.05) in the blastocyst rates between the Control group (44.9%) and the other treatments, the embryo production was lower (P<0.05) in Forsk 10.0 group (38.8%) compared to Forsk 2.5 (50.5%) and Forsk 5.0 (54.7%) groups. The intracytoplasmic lipid content (expressed in arbitrary units of pixels) in blastocysts from the Control group (1.00±0.03) was similar (P>0.05) to that found in Forsk 2.5 (0.92±0.03) and Forsk 10.0 groups (1.06±0.03) groups; however the lipid accumulation in blastocysts from Forsk 5.0 group (0.82±0.04) was lower than in the Control group (P<0.05). Based on these results, Forsk 5.0 treatment was tested for cryotolerance and it was observed that the blastocoel re-expansion rate evaluated 24 hours after warming was greater (P<0.05) in Forsk 5.0 group (72.2%) compared to the Control group (46.2%). In conclusion, pre-treatment with forskolin at a concentration of 5.0 μM for 24 hours before vitrification is effective in reducing the intracytoplasmic lipid content and, consequently, improves cryotolerance of IVP bovine embryos. 650 $aforskolin 650 $aBovino 650 $aEmbrião 653 $aAcúmulo lipídico intracitoplasmático 653 $aCriotolerância 653 $aLipídio intracitoplasmático 653 $aProdução in vitro 653 $aVitrificação 700 1 $aDALL'ACQUA, P. C. 700 1 $aAMBROGI, M. 700 1 $aLEÃO, B. C. S. 700 1 $aROCHA-FRIGONI, N. A. S. 700 1 $aMINGOTI, G. Z. 773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 37, n. 4, p. 395-400, abril 2017.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|