Data/hora: 01/06/2024 13:45:00

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 04/04/2016
Data da última atualização: | 01/08/2018
Autoria: | BRANCO, E.; SILVA, K. S. M.; ASSIS, M. de F.; OLIVEIRA, E. H. C. de; LIMA, A. R. de; AQUINO, A. C. G.; CABRAL, R.; MIGLINO, M. A.
Afiliação: | ÉRICA BRANCO, ISPA/UFRA; KLENA SARGES MARRUAZ SILVA, EC/CENP; MARIA FÁTIMA ASSIS, EC/CENP; EDIVALDO HERCULANO CORRÊA DE OLIVEIRA, EC/CENP; ANA RITA DE LIMA, ISPA/UFRA; ANA CLEIDE GUIMBAL DE AQUINO, ISPA/UFRA; ROSA CABRAL, ISPA/UFRA; MARIA ANGELICA MIGLINO, FMVZ/USP.
Título: | Protocol for collection and deparation of bone marrow mononuclear cells in Chlorocebus aethiops.
Ano de publicação: | 2016
Fonte/Imprenta: | Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 2, p. 119-122, fev. 2016.
Idioma: | Inglês
Conteúdo: | Chlorocebus aethiops is a species of non-human primate frequently used in biomedical research. Some research involves this species as an experimental model for various diseases and possible treatment with stem cells. The bone marrow is one of the main sources of these cells and provides easy access. The aim of this study was to standardize the protocol of collection and separation of bone marrow in C. aethiops. Ten animals were submitted to puncture of bone marrow with access to the iliac crest and cell separation by density gradient. The bone marrow of C. aethiops had an average of 97% viability. From the results achieved, we can conclude that C. aethiops is an excellent model to obtain and isolate mononuclear cells from bone marrow, fostering several studies in the field of cell therapy.
Palavras-Chave: | Célula mononuclear; Célula-tronco; Mononuclear cells.
Thesagro: | Medula óssea.
Thesaurus Nal: | bone marrow; Cercopithecus aethiops; Stem cells.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/141953/1/Protocol-for-collection-and-separation.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (59271-1) - UPE - AP - PP - 636.08905 - P474 -  - DIS