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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
19/01/2004 |
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Data da última atualização: |
27/07/2009 |
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Autoria: |
AZZOLINI, S. S.; SASAKI, S. D.; TORQUATO, R. J. S.; ANDREOTTI, R.; ANDREOTTI, E.; TANAKA, A. S. |
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Afiliação: |
Universidade Federal de São Paulo. Escola Paulista de Medicina (São Paulo, SP). |
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Título: |
Rhipicephalus sanguineus trypsin inhibitors present in the tick larvae: isolation, characterization, and parcial primary structure determination. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Archives of Biochemistry and Biophysics, New York, n. 417, p. 176-182, 2003. |
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Idioma: |
Inglês |
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Notas: |
CNPGC. |
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Conteúdo: |
Blood sucking animals are a rich source of proteinase inhibitors mainly those thayt interfere in their host hemostatic systems. The tick Rhipicephalus anguineus is an ectoparasite of dogs and other animals. The aims of thos work ere the purification and characterization of serine proteinase inhibitors present in R. sanguineus larvae (Rs TI). The inhibitores (RsTI) were isolated by affinity chromatography on trypsin-sepharose and ion exchange chromatographies in Resource Q and Mono S columns. These Rs'TIs were separated in around 12 different protein peaks, when they showed molecular masses between 8 and 18 kDa, by SDS-PAGE. Purified Rs TIs presented differences in the specificility for differen serine proteinases. s TQ2 was, better inhibitor than RsTIQ7 and RsTISS for neutrophil elastase, plasmin and HuPK with dissociation constants (Kii) of 1.3, 3.2 and 22nM, respectively. Other inhibitors sus as RsTIQ7, Rs TIS3, and affected neutrophil elastase and plasmin with Ki in the nM range The RsTIQ2, RsTIQ7, and Rs TIS5 amino acid sequence data allowed classifying them as members of the Kunitz-type serine proteinas inhibitor family, even though the RsTI role still unknown. Our present results showed that serine proteinase inhibitors from R sangguineus are similar to inhibitors from Boophilus microplus other hard tick species, suggesting a similar role of these inhibitors in hard tick species and also as a potential tool to generate or improve vaccine against different ectoparasites with an unique antigen. MenosBlood sucking animals are a rich source of proteinase inhibitors mainly those thayt interfere in their host hemostatic systems. The tick Rhipicephalus anguineus is an ectoparasite of dogs and other animals. The aims of thos work ere the purification and characterization of serine proteinase inhibitors present in R. sanguineus larvae (Rs TI). The inhibitores (RsTI) were isolated by affinity chromatography on trypsin-sepharose and ion exchange chromatographies in Resource Q and Mono S columns. These Rs'TIs were separated in around 12 different protein peaks, when they showed molecular masses between 8 and 18 kDa, by SDS-PAGE. Purified Rs TIs presented differences in the specificility for differen serine proteinases. s TQ2 was, better inhibitor than RsTIQ7 and RsTISS for neutrophil elastase, plasmin and HuPK with dissociation constants (Kii) of 1.3, 3.2 and 22nM, respectively. Other inhibitors sus as RsTIQ7, Rs TIS3, and affected neutrophil elastase and plasmin with Ki in the nM range The RsTIQ2, RsTIQ7, and Rs TIS5 amino acid sequence data allowed classifying them as members of the Kunitz-type serine proteinas inhibitor family, even though the RsTI role still unknown. Our present results showed that serine proteinase inhibitors from R sangguineus are similar to inhibitors from Boophilus microplus other hard tick species, suggesting a similar role of these inhibitors in hard tick species and also as a potential tool to generate or improve vaccine against different ect... Mostrar Tudo |
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Palavras-Chave: |
Ensyme inhibitors; Trypsina. |
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Thesagro: |
Carrapato; Imunologia; Inibidor de Enzima; Protease; Tripsina. |
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Thesaurus Nal: |
immunology; Rhipicephalus sanguineus; ticks. |
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Categoria do assunto: |
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Marc: |
LEADER 02445naa a2200313 a 4500
001 1325436
005 2009-07-27
008 2003 bl --- 0-- u #d
100 1 $aAZZOLINI, S. S.
245 $aRhipicephalus sanguineus trypsin inhibitors present in the tick larvae$bisolation, characterization, and parcial primary structure determination.
260 $c2003
500 $aCNPGC.
520 $aBlood sucking animals are a rich source of proteinase inhibitors mainly those thayt interfere in their host hemostatic systems. The tick Rhipicephalus anguineus is an ectoparasite of dogs and other animals. The aims of thos work ere the purification and characterization of serine proteinase inhibitors present in R. sanguineus larvae (Rs TI). The inhibitores (RsTI) were isolated by affinity chromatography on trypsin-sepharose and ion exchange chromatographies in Resource Q and Mono S columns. These Rs'TIs were separated in around 12 different protein peaks, when they showed molecular masses between 8 and 18 kDa, by SDS-PAGE. Purified Rs TIs presented differences in the specificility for differen serine proteinases. s TQ2 was, better inhibitor than RsTIQ7 and RsTISS for neutrophil elastase, plasmin and HuPK with dissociation constants (Kii) of 1.3, 3.2 and 22nM, respectively. Other inhibitors sus as RsTIQ7, Rs TIS3, and affected neutrophil elastase and plasmin with Ki in the nM range The RsTIQ2, RsTIQ7, and Rs TIS5 amino acid sequence data allowed classifying them as members of the Kunitz-type serine proteinas inhibitor family, even though the RsTI role still unknown. Our present results showed that serine proteinase inhibitors from R sangguineus are similar to inhibitors from Boophilus microplus other hard tick species, suggesting a similar role of these inhibitors in hard tick species and also as a potential tool to generate or improve vaccine against different ectoparasites with an unique antigen.
650 $aimmunology
650 $aRhipicephalus sanguineus
650 $aticks
650 $aCarrapato
650 $aImunologia
650 $aInibidor de Enzima
650 $aProtease
650 $aTripsina
653 $aEnsyme inhibitors
653 $aTrypsina
700 1 $aSASAKI, S. D.
700 1 $aTORQUATO, R. J. S.
700 1 $aANDREOTTI, R.
700 1 $aANDREOTTI, E.
700 1 $aTANAKA, A. S.
773 $tArchives of Biochemistry and Biophysics, New York$gn. 417, p. 176-182, 2003.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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