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| 21. |  | BATISTA, J. S. da S.; BARCELLOS, F. G.; MENNA, P.; BALLATI, P. A.; HUNGRIA, M. Confirmação da transferência horizontal de genes simbióticos entre gêneros distintos de rizóbios pela caracterização da estirpe de Sinorhizobium fredii CPAC 402, isolada de nódulo de soja nos cerrados. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 31., 2007, Gramado. Conquistas e desafios da ciência do solo brasileira : livro de resumos... Gramado: UFRGS: SBCS, 2007. 1 CD-ROM. Pdf. 7324-1775.| Biblioteca(s): Embrapa Soja. |
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| 24. | | MENNA, P.; HUNGRIA, M.; BARCELLOS, F. G.; BANGEL, E. V.; HESS, P. N.; MARTÍNEZ-ROMERO, E. Molecular phylogeny based on the 16S rRNA gene of elite rhizobial strains used in Brazilian commercial inoculants. Systematic and Applied Microbiology, Stuttgart, v.29, n. 4, p. 315-332, Jun. 2006.| Biblioteca(s): Embrapa Soja. |
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| 25. | | DELAMUTA, J. R. M.; RIBEIRO, R. A.; MENNA, P.; BANGEL, E. V.; HUNGRIA, M. Multilocus sequence analysis (MLSA) of Bradyrhizobium strains: revealing high diversity of tropical diazotrophic symbiotic bacteria. Brazilian Journal of Microbiology, São Paulo, v. 43, n.2, p. 698-710, Apr./June 2012.| Biblioteca(s): Embrapa Soja. |
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| 29. | | BARCELLOS, F. G.; MENNA, P.; BATISTA, J. S. da S.; HUNGRIA, M. Transferência horizontal de genes entre diferentes espécies de rizóbios nos solos dos cerrados. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 27.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 11.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 9.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 6., 2006, Bonito, MS. A busca das raízes: anais. Dourados: Embrapa Agropecuária Oeste, 2006. 1 CD-ROM. (Embrapa Agropecuária Oeste. Documentos, 82).| Biblioteca(s): Embrapa Soja. |
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| 31. | | BATISTA, J. S. S.; MENNA, P.; BARCELLOS, F. G.; MENDES, I. C.; HUNGRIA, M. Variabilidade de estirpes de Bradyrhizobium japonicum e B. elkanii após sete anos de introdução em um solo dos cerrados. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 27.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 11.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 9.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 6., 2006, Bonito, MS. A busca das raízes: anais. Dourados: Embrapa Agropecuária Oeste, 2006. 1 CD-ROM. (Embrapa Agropecuária Oeste. Documentos, 82).| Biblioteca(s): Embrapa Soja. |
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| 32. | | BARCELLOS, F. G.; DELAMUTA, J. R. M.; HUNGRIA, M.; MENNA, P.; BATISTA, J. S. da S.; RIBEIRO, R. A. Taxonomia bacteriana - aspectos atuais e perspectivas. In: YAMADA-OGATTA, S. F.; NAKAZATO, G.; FURNALETTO, M. C.; NOGUEIRA, M. A. (Org.). Tópicos especiais em microbiologia. Londrina: UEL, 2015. p. 7-28.| Biblioteca(s): Embrapa Soja. |
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| 33. | | HUNGRIA, M.; MENNA, P.; GERMANO, M. G.; CHUEIRE, L. M. O.; BANGEL, E. V.; CAMPO, R. J. Diversity of a Brazilian collection of rhizobial strains. In: WANG, Y. P.; LIN, M.; TIAN, Z. X.; ELMERICH, C.; NEWTON. W. E. (Ed). Biological nitrogen fixation, sustainable agriculture and the environment. Dordrecht: Springer, 2005. p. 413. Proceedingsof the 14th International Nitrogen Fixation Congress.| Biblioteca(s): Embrapa Soja. |
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| 34. | | MENNA, P.; BARCELLOS, F. G.; BATISTA, J. S. da S.; BANGEL, E.; CAMPO, R. J.; HUNGRIA, M. Análise filogenética de rizóbios, utilizados em inoculantes comerciais brasileiros, com base no sequenciamento do gene ribossomal 16S. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 2005, Londrina. Resumos expandidos. Londrina: Embrapa Soja, 2005. p.128-133. (Embrapa Soja. Documentos, 268).| Biblioteca(s): Embrapa Soja. |
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| 35. | | VESPERO, E. C.; MENNA, P.; BARCELLOS, F. G.; PERUGINI, M. R. E.; CUNHA, M. H.; SARIDAKIS, H. O. Epidemiologia molecular de isolados clínicos de Klebsiella pneumoniae produtora de esbl. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 24., 2007, Brasília, DF. Anais... Brasília, DF: Sociedade Brasileira de Microbiologia, 2007. 1 CD-ROM. Nome correto do quinto autor HUNGRIA, M.| Biblioteca(s): Embrapa Soja. |
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| 36. | | BATISTA, J. S.; BARCELLOS, F. G.; MENNA, P.; CHUEIRE, L. M. O.; MENDES, I. C.; HUNGRIA, M. Evidências em nível molecular da importância da reinoculação da soja. In: CONGRESSO BRASILEIRO DE SOJA, 4., 2006, Londrina. Resumos... Londrina: Embrapa Soja, 2006. p. 180. Organizado por Odilon Ferreira Saraiva, Simone Ery Grosskopf.| Biblioteca(s): Embrapa Soja. |
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| 37. | | BATISTA, J. S.; HUNGRIA, M.; BARCELLOS, F. G.; MENNA, P.; CHUEIRE, L. M. O.; MENDES, I. C. Evidências em nível molecular da importância da reinoculação da soja. In: CONGRESO DE SOJA DEL MERCOSUR, 3., 2006, Rosário. Mercosoja 2006: mesas científico-técnicas, resúmenes expandidos / comunicaciones. Rosário: Associación de la Cadena de Soja Argentina, 2006. p. 673.| Biblioteca(s): Embrapa Soja. |
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| 38. | | HUNGRIA, M.; GERMANO, M. G.; MENNA, P.; GALLI-TERASAWA, L. V.; CHUEIRE, L. M. de O.; BANGEL, E. V.; MENDES, I. C.; CAMPO, R. J. Diversity of rhizobia in brazilian soils. In: INTERNATIONAL CONGRESS ON NITROGEN FIXATION, 14., Beijing. Program and abstract book... [S.l.: s.n., 2004]. p. 131.| Biblioteca(s): Embrapa Soja. |
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| 39. | | HUNGRIA, M.; BARCELLOS, F. G.; MENDES, I. C.; CHUEIRE, L. M. de O.; RIBEIRO, R. A.; BATISTA, J. S. da S.; MENNA, P.; DELAMUTA, J. R. M. Introdução, estabelecimento e adaptação de Bradirrizóbios simbiontes da soja em a solos brasileiros. In: YAMADA-OGATTA, S. F.; NAKAZATO, G.; FURNALETTO, M. C.; NOGUEIRA, M. A. (Org.). Tópicos especiais em microbiologia. Londrina: UEL, 2015. p. 243-261.| Biblioteca(s): Embrapa Soja. |
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| 40. | | BANGEL, E. V.; MEYER, J. V.; OLIVEIRA, A. M. R.; SILVA, G. M.; ÁVILA, L. D.; MESSA, L.; AQUINO, A. S.; HUNGRIA, M.; CHUEIRE, L. M.; MENNA, P. Metodologias de caracterização para o monitoramento das estirpes liofillzadas SEMIA, recomendadas na produção de inoculantes. In: REUNIÃO DA REDE DE LABORATÓRIOS PARA RECOMENDAÇÃO, PADRONIZAÇÃO E DIFUSÃO DE TECNOLOGIA DE INOCULANTES MICROBIANOS DE INTERESSE AGRÍCOLA (RELARE), 13., 2006, Londrina. Anais... Londrina: Embrapa Soja, 2007. p. 43. (Embrapa Soja. Documentos, 290). Organizado por Rubens José Campo, Mariângela Hungria.| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 47 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
22/12/2005 |
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Data da última atualização: |
22/12/2005 |
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Autoria: |
HUNGRIA, M.; MENNA, P.; GERMANO, M. G.; CHUEIRE, L. M. O.; BANGEL, E. V.; CAMPO, R. J. |
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Título: |
Diversity of a Brazilian collection of rhizobial strains. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
In: WANG, Y. P.; LIN, M.; TIAN, Z. X.; ELMERICH, C.; NEWTON. W. E. (Ed). Biological nitrogen fixation, sustainable agriculture and the environment. Dordrecht: Springer, 2005. p. 413. |
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Idioma: |
Inglês |
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Notas: |
Proceedingsof the 14th International Nitrogen Fixation Congress. |
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Conteúdo: |
The Leguminosae is one of the largest plant families with over 18,000 species classified into around 650 genera: most are capable of effective symbioses with rhizobial strains. Howerver, despite reports of high strain diversity, especially in the tropics, there are less than 40 described rhizobial species. Ribosomal sequences, emphasinzing 16S rRNA gene ( 1.5 kb), are the method of choice for tracing bacteria phylogenies. Speciation of genera can be enhanced by analaysis of other ribosomal genes because both the 23S rRNA ( 2.3 kb) and the 16S-23S rRNA intergenic space (ITS) show higher variability than 16S rRNA. Analyses of rhizobial ribosomal genes have used RFLP-PCR, as well as partial or complete sequencing of bases. In Brazil, there are 200 rhizobial strains recommended for 95 legume species, but little is known of their genetic diversity. A Brazilian culture collection of 119 strains of Bradyrhizobium, isolated from thirty-three legume species, representing nine tribes and all three subfamilies, were analyzed by RFLP-PCR of the 16S rRNA, 23S rRNA and ITS region, each with three restriction enzymes. 43 of those atrains are recommended in Brazil as the most effective for 31 host legumes. For the 16S rRNA gene, reference strains of B. japonicum fit into two major clusters at 50% similarity, whereas two other cluster (at 53% similarity) were composed of strains of B. elkanii; all strains were joined at a final level of similarity of 28%. The higher variability in the ITS and 23rRNA resulted in final grouping at very low level of similarities, 27% and 16% respectively. Considering the three ribosomal regions, two great groups were visualized, related to B. japonicum (with most strains from soybean) and B. elkanii (most strains from indigenous legumes), at similarity levels of 54 and 46%, respectively, and at least two new clusters that might represent new species. The complete 16S rRNA sequence for another set of 80 rhizobial strains from 30 different legume species (23 recommended as most effective) were clustered within the genera Rhizobium, Sinorhizobium, Mesorhizobium and Bradyrhizobium. Three strains, recommended for Clitoria fairchildian, Piptadenia gonoacantha and Ormosia nitida, were clustered with Burkholderia sp. strain TJ182. This collection of strains indicates an extremely high level of genetic diversity in the tropics. MenosThe Leguminosae is one of the largest plant families with over 18,000 species classified into around 650 genera: most are capable of effective symbioses with rhizobial strains. Howerver, despite reports of high strain diversity, especially in the tropics, there are less than 40 described rhizobial species. Ribosomal sequences, emphasinzing 16S rRNA gene ( 1.5 kb), are the method of choice for tracing bacteria phylogenies. Speciation of genera can be enhanced by analaysis of other ribosomal genes because both the 23S rRNA ( 2.3 kb) and the 16S-23S rRNA intergenic space (ITS) show higher variability than 16S rRNA. Analyses of rhizobial ribosomal genes have used RFLP-PCR, as well as partial or complete sequencing of bases. In Brazil, there are 200 rhizobial strains recommended for 95 legume species, but little is known of their genetic diversity. A Brazilian culture collection of 119 strains of Bradyrhizobium, isolated from thirty-three legume species, representing nine tribes and all three subfamilies, were analyzed by RFLP-PCR of the 16S rRNA, 23S rRNA and ITS region, each with three restriction enzymes. 43 of those atrains are recommended in Brazil as the most effective for 31 host legumes. For the 16S rRNA gene, reference strains of B. japonicum fit into two major clusters at 50% similarity, whereas two other cluster (at 53% similarity) were composed of strains of B. elkanii; all strains were joined at a final level of similarity of 28%. The higher variability in the ITS ... Mostrar Tudo |
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Marc: |
LEADER 03068naa a2200193 a 4500
001 1468692
005 2005-12-22
008 2005 bl --- 0-- u #d
100 1 $aHUNGRIA, M.
245 $aDiversity of a Brazilian collection of rhizobial strains.
260 $c2005
500 $aProceedingsof the 14th International Nitrogen Fixation Congress.
520 $aThe Leguminosae is one of the largest plant families with over 18,000 species classified into around 650 genera: most are capable of effective symbioses with rhizobial strains. Howerver, despite reports of high strain diversity, especially in the tropics, there are less than 40 described rhizobial species. Ribosomal sequences, emphasinzing 16S rRNA gene ( 1.5 kb), are the method of choice for tracing bacteria phylogenies. Speciation of genera can be enhanced by analaysis of other ribosomal genes because both the 23S rRNA ( 2.3 kb) and the 16S-23S rRNA intergenic space (ITS) show higher variability than 16S rRNA. Analyses of rhizobial ribosomal genes have used RFLP-PCR, as well as partial or complete sequencing of bases. In Brazil, there are 200 rhizobial strains recommended for 95 legume species, but little is known of their genetic diversity. A Brazilian culture collection of 119 strains of Bradyrhizobium, isolated from thirty-three legume species, representing nine tribes and all three subfamilies, were analyzed by RFLP-PCR of the 16S rRNA, 23S rRNA and ITS region, each with three restriction enzymes. 43 of those atrains are recommended in Brazil as the most effective for 31 host legumes. For the 16S rRNA gene, reference strains of B. japonicum fit into two major clusters at 50% similarity, whereas two other cluster (at 53% similarity) were composed of strains of B. elkanii; all strains were joined at a final level of similarity of 28%. The higher variability in the ITS and 23rRNA resulted in final grouping at very low level of similarities, 27% and 16% respectively. Considering the three ribosomal regions, two great groups were visualized, related to B. japonicum (with most strains from soybean) and B. elkanii (most strains from indigenous legumes), at similarity levels of 54 and 46%, respectively, and at least two new clusters that might represent new species. The complete 16S rRNA sequence for another set of 80 rhizobial strains from 30 different legume species (23 recommended as most effective) were clustered within the genera Rhizobium, Sinorhizobium, Mesorhizobium and Bradyrhizobium. Three strains, recommended for Clitoria fairchildian, Piptadenia gonoacantha and Ormosia nitida, were clustered with Burkholderia sp. strain TJ182. This collection of strains indicates an extremely high level of genetic diversity in the tropics.
700 1 $aMENNA, P.
700 1 $aGERMANO, M. G.
700 1 $aCHUEIRE, L. M. O.
700 1 $aBANGEL, E. V.
700 1 $aCAMPO, R. J.
773 $tIn: WANG, Y. P.; LIN, M.; TIAN, Z. X.; ELMERICH, C.; NEWTON. W. E. (Ed). Biological nitrogen fixation, sustainable agriculture and the environment. Dordrecht: Springer, 2005. p. 413.
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