|
|
Registro Completo |
Biblioteca(s): |
Embrapa Clima Temperado. |
Data corrente: |
16/12/2014 |
Data da última atualização: |
17/12/2014 |
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
Autoria: |
REISSER JUNIOR, C.; SIMÕES, F. |
Afiliação: |
CARLOS REISSER JUNIOR, CPACT; FABIANO SIMÕES, UERGS. |
Título: |
Irrigação. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
In: RASEIRA, M. do C. B.; PEREIRA, J. F. M.; CARVALHO, F. L. C. (Ed.). Pessegueiro. Brasília, DF: Embrapa, 2014. p. 329-353. |
Idioma: |
Português |
Palavras-Chave: |
Estresse hídrico. |
Thesagro: |
Irrigação; Pessego. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00463naa a2200157 a 4500 001 2002704 005 2014-12-17 008 2014 bl uuuu u00u1 u #d 100 1 $aREISSER JUNIOR, C. 245 $aIrrigação. 260 $c2014 650 $aIrrigação 650 $aPessego 653 $aEstresse hídrico 700 1 $aSIMÕES, F. 773 $tIn: RASEIRA, M. do C. B.; PEREIRA, J. F. M.; CARVALHO, F. L. C. (Ed.). Pessegueiro. Brasília, DF: Embrapa, 2014. p. 329-353.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Clima Temperado (CPACT) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
20/09/2021 |
Data da última atualização: |
28/08/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
RAMOS, E. T. de A.; MENESES, C. H. S. G.; VIDAL, M. S.; BALDANI, J. I. |
Afiliação: |
ELIZABETH TEIXEIRA DE ALMEIDA RAMOS, UFRRJ; CARLOS HENRIQUE SALVINO GADELHA MENESES, UEPB; MARCIA SOARES VIDAL, CNPAB; JOSE IVO BALDANI, CNPAB. |
Título: |
Characterisation and action mode of Gluconacin, a bacteriocin with antimicrobial activity against Xanthomonas albilineans. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Annals of Applied Biology, Annals of Applied Biology, v. 180, p. 163-175. 2022. |
DOI: |
DOI: 10.1111/aab.12721 |
Idioma: |
Inglês |
Conteúdo: |
Gluconacin, a bacteriocin produced by Gluconacetobacter diazotrophicus PAL5, has previously shown a large spectrum of inhibitory activity against beneficial and phytopatho- genic bacteria. The present study proposes a three-dimensional (3D) structure for Gluconacin based on modelling and describes some physicochemical characteristics and the effect of the peptide on cells of the phytopathogenic bacterium Xanthomonas albilineans ICMP196. The Gluconacin 3D structural model results demonstrated the 7 ?helices and 11 ?-sheets. Growth inhibition of the indicator microorganism, X. albilineans, occurred in the first moments of contact with Gluconacin (0.25 ?g ?l 1 ). Treatment of bacterial cells caused significant loss of inorganic phosphate and UV-absorbing materials. Scanning electron microscopy images showed that Gluconacin-treated X. lbilineans cells were fully lysed. These results suggest that the mode of action of this peptide involves altered membrane integrity and increased permeability, resulting in complete cell lysis. Physicochemical characterisation demonstrated stability of biological activity under high temperatures, including autoclaving conditions and low pH. The activity was reduced after treatment with proteases and some surfactants and abolished with ethylenediaminetetraacetic acid (EDTA). The 3D conformation model indicates the possibility of a hydrophobic region, which could possibly interact directly with the cell membrane of target bacteria. In conclusion, the ability of Gluconacin to inhibit growth of phytopathogens of agricultural importance opens up new opportunities for biotechnological applications and, consequently, a reduction in the use of pesticides. MenosGluconacin, a bacteriocin produced by Gluconacetobacter diazotrophicus PAL5, has previously shown a large spectrum of inhibitory activity against beneficial and phytopatho- genic bacteria. The present study proposes a three-dimensional (3D) structure for Gluconacin based on modelling and describes some physicochemical characteristics and the effect of the peptide on cells of the phytopathogenic bacterium Xanthomonas albilineans ICMP196. The Gluconacin 3D structural model results demonstrated the 7 ?helices and 11 ?-sheets. Growth inhibition of the indicator microorganism, X. albilineans, occurred in the first moments of contact with Gluconacin (0.25 ?g ?l 1 ). Treatment of bacterial cells caused significant loss of inorganic phosphate and UV-absorbing materials. Scanning electron microscopy images showed that Gluconacin-treated X. lbilineans cells were fully lysed. These results suggest that the mode of action of this peptide involves altered membrane integrity and increased permeability, resulting in complete cell lysis. Physicochemical characterisation demonstrated stability of biological activity under high temperatures, including autoclaving conditions and low pH. The activity was reduced after treatment with proteases and some surfactants and abolished with ethylenediaminetetraacetic acid (EDTA). The 3D conformation model indicates the possibility of a hydrophobic region, which could possibly interact directly with the cell membrane of target bacteria. In conclusion, th... Mostrar Tudo |
Palavras-Chave: |
Antimicrobial peptide; Bactericidal effect. |
Thesaurus NAL: |
Biological control; Gluconacetobacter diazotrophicus. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02455naa a2200217 a 4500 001 2134566 005 2023-08-28 008 2022 bl uuuu u00u1 u #d 024 7 $aDOI: 10.1111/aab.12721$2DOI 100 1 $aRAMOS, E. T. de A. 245 $aCharacterisation and action mode of Gluconacin, a bacteriocin with antimicrobial activity against Xanthomonas albilineans.$h[electronic resource] 260 $c2022 520 $aGluconacin, a bacteriocin produced by Gluconacetobacter diazotrophicus PAL5, has previously shown a large spectrum of inhibitory activity against beneficial and phytopatho- genic bacteria. The present study proposes a three-dimensional (3D) structure for Gluconacin based on modelling and describes some physicochemical characteristics and the effect of the peptide on cells of the phytopathogenic bacterium Xanthomonas albilineans ICMP196. The Gluconacin 3D structural model results demonstrated the 7 ?helices and 11 ?-sheets. Growth inhibition of the indicator microorganism, X. albilineans, occurred in the first moments of contact with Gluconacin (0.25 ?g ?l 1 ). Treatment of bacterial cells caused significant loss of inorganic phosphate and UV-absorbing materials. Scanning electron microscopy images showed that Gluconacin-treated X. lbilineans cells were fully lysed. These results suggest that the mode of action of this peptide involves altered membrane integrity and increased permeability, resulting in complete cell lysis. Physicochemical characterisation demonstrated stability of biological activity under high temperatures, including autoclaving conditions and low pH. The activity was reduced after treatment with proteases and some surfactants and abolished with ethylenediaminetetraacetic acid (EDTA). The 3D conformation model indicates the possibility of a hydrophobic region, which could possibly interact directly with the cell membrane of target bacteria. In conclusion, the ability of Gluconacin to inhibit growth of phytopathogens of agricultural importance opens up new opportunities for biotechnological applications and, consequently, a reduction in the use of pesticides. 650 $aBiological control 650 $aGluconacetobacter diazotrophicus 653 $aAntimicrobial peptide 653 $aBactericidal effect 700 1 $aMENESES, C. H. S. G. 700 1 $aVIDAL, M. S. 700 1 $aBALDANI, J. I. 773 $tAnnals of Applied Biology, Annals of Applied Biology$gv. 180, p. 163-175. 2022.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Agrobiologia (CNPAB) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|