Data/hora: 18/06/2024 14:15:44

Biblioteca(s): | Embrapa Agroenergia; Embrapa Agroindústria de Alimentos.
Data corrente: | 19/09/2017
Data da última atualização: | 08/12/2017
Autoria: | TAMIETTI, M. S.; MIDORIKAWA, G. E. O.; MENDES, T. D.; DAMASO, M. C. T.; BOM, E. P. S.; SILVA, A. S.; GOTTSCHALK, L. M. F.; NORONHA, E. F.; FAVARO, L. C. de L.
Afiliação: | MARIANA S. TAMIETTI, UNB; GLÁUCIA E. O. MIDORIKAWA; THAIS DEMARCHI MENDES, CNPAE; MONICA CARAMEZ TRICHES DAMASO, CNPAE; ELBA P. S. BOM, UFRJ; AYLA S. SILVA, INT; LEDA MARIA FORTES GOTTSCHALK, CTAA; ELIANE F. NORONHA, UNB; LEIA CECILIA DE LIMA FAVARO, CNPAE.
Título: | Enhancement of Trichoderma harzianum CFAM-422 for cellulase and hemicellulase production by deletion of the carbon catabolite repressor gene cre1.
Ano de publicação: | 2017
Fonte/Imprenta: | In: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 21.; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, 12., 2017, Aracaju, SE. [Proceedings ...]. São Paulo: Associação Brasileira de Engenharia Química, 2017.
Páginas: | Não paginado.
ISSN: | 2447-2816
Idioma: | Inglês
Notas: | SINAFERM; SHEB. 3 a 6 de setembro. Seção Trabalhos. Ref. 59019.
Conteúdo: | Carbon catabolite repression (CCR) is a mechanism by which microorganisms can utilize preferably highly energetic compounds over those of difficult degradation. For Trichoderma reesei, the protein that acts as repressor in the presence of glucose is CRE1. In this project, we aim to delete cre1 gene in Trichoderma harzianum CFAM-422 and obtain mutants with enhanced production of biomass degrading enzymes. Disruption of cre1 in T. harzianum CFAM-422 was performed by gene replacement of cre1 for hph (hygromycin B phosphotransferase) via homologous recombination. Hygromycin resistant mutants and parental strains enzyme production was evaluated in both inductive and repressive conditions in four different carbon sources. Enzymatic indexes (EI) were determined and compared. All genetically stable transformants showed increased enzymatic index under inductive conditions and modest inhibition under repressive conditions for most carbon sources, indicating that the deletion of cre1 in T. harzianum can be beneficial to cellulase and hemicellulase production with reduced product inhibition.
Palavras-Chave: | Carbon Catabolite Repression; Cellulolytic and hemicellulolytic enzymes; Processos de hidrolise enzimática de biomassa.
Thesagro: | Celulase; Trichoderma Harzianum.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/164843/1/galoa-proceedings-sinaferm-2017-59019-enhancement-of-t.pdf
Registro original: | Embrapa Agroindústria de Alimentos (CTAA)

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