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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
26/04/2005 |
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Data da última atualização: |
27/02/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
LEVY, S. M.; FALLEIROS, A. M. F.; MOSCARDI, F.; GREGÓRIO, E. A. |
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Afiliação: |
CNPSO. |
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Título: |
Is the Nucleopolyhedrovirus of Anticarsia gemmatalis (AgMNPV) ineffective to infect AgMNPV resistant host larva midgut cells? |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
In: ANNUAL MEETING SOCIETY FOR INVERTEBRATE PATHOLOGY, 36., 2003, Burlington. Program and abstracts... Burlington, 2003. |
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Páginas: |
p. 81-82. |
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Idioma: |
Inglês |
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Conteúdo: |
Anticarsia gemmatalis, is a key pests of soybean in Brazil. It has been controlled by a nucleopolyhedrovirus (AgMNPV), which is widely used as a microbial insecticide in the country. The constant and increasing use of this biological insecticide in some regions have caused concerns about the possibility of selection of viral resistant populations. Although this phenomenon has not been detected in the field yet, a laboratory A. gemmatalis populations has been selected for high resistance to the AgMNPV. It is known that the midgut is considered one of the most important barriers against viral invasion, before systemic infections can be caused in various tissues if the virus succeeds in reaching the host larva hemocele. Our work aimed to verify whether or not the AgMNPV invades and infects the midgut cells of resistant larvae, comparing the ultrastructure of the midgut epithelial cells from the susceptible (SL) and resistant (RL) A. gemmatalis infected larvae. The susceptible and resistant strains of A. gemmatalis were reared on artificial diet, under laboratory-controlled conditions at Embrapa Soja, Londrina-PR, Brazil. The AgMNPV used as inoculum was incorporated into the insect diet at 60,000 occlusion bodies/ml of diet. The midguts were collected up to 120h post infection, processed and analyzed under transmission electron microscopy. The columnar cell were the most affected ones among the different midgut epithelial cells. In the SL, these cells showed many morphological signs of cellular damage from 24 hours after infection, mainly at the proximal midgut region. However, in RL these cells exhibited minor morphological damage up to 120 hours. Polyhedra were visualized in the midgut of both SL an RL from 96 hours of infection, but the amount of viral structures were always higher in SL, affecting the midgut epithelial and tracheal cells, as well as attached hemocytes (mainly plasmatocytes and granulocytes). Our results showed that the AgMNPV, in fact, invaded RL cells. However, the virus did not affect these insects as they did with the susceptible ones, allowing development and survival of resistant larvae. The mechanism interfering with the virus infection progress in midgut cells of RL it still unknown. This work has been supported by FAPESP and PRONEX (MCT/Finep/CNPq). MenosAnticarsia gemmatalis, is a key pests of soybean in Brazil. It has been controlled by a nucleopolyhedrovirus (AgMNPV), which is widely used as a microbial insecticide in the country. The constant and increasing use of this biological insecticide in some regions have caused concerns about the possibility of selection of viral resistant populations. Although this phenomenon has not been detected in the field yet, a laboratory A. gemmatalis populations has been selected for high resistance to the AgMNPV. It is known that the midgut is considered one of the most important barriers against viral invasion, before systemic infections can be caused in various tissues if the virus succeeds in reaching the host larva hemocele. Our work aimed to verify whether or not the AgMNPV invades and infects the midgut cells of resistant larvae, comparing the ultrastructure of the midgut epithelial cells from the susceptible (SL) and resistant (RL) A. gemmatalis infected larvae. The susceptible and resistant strains of A. gemmatalis were reared on artificial diet, under laboratory-controlled conditions at Embrapa Soja, Londrina-PR, Brazil. The AgMNPV used as inoculum was incorporated into the insect diet at 60,000 occlusion bodies/ml of diet. The midguts were collected up to 120h post infection, processed and analyzed under transmission electron microscopy. The columnar cell were the most affected ones among the different midgut epithelial cells. In the SL, these cells showed many morphological s... Mostrar Tudo |
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Thesagro: |
Baculovirus; Controle Biológico; Praga de Planta; Resistência; Soja. |
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Thesaurus Nal: |
Soybeans. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03077nam a2200229 a 4500
001 1467873
005 2020-02-27
008 2003 bl uuuu u00u1 u #d
100 1 $aLEVY, S. M.
245 $aIs the Nucleopolyhedrovirus of Anticarsia gemmatalis (AgMNPV) ineffective to infect AgMNPV resistant host larva midgut cells?$h[electronic resource]
260 $aIn: ANNUAL MEETING SOCIETY FOR INVERTEBRATE PATHOLOGY, 36., 2003, Burlington. Program and abstracts... Burlington$c2003
300 $ap. 81-82.
520 $aAnticarsia gemmatalis, is a key pests of soybean in Brazil. It has been controlled by a nucleopolyhedrovirus (AgMNPV), which is widely used as a microbial insecticide in the country. The constant and increasing use of this biological insecticide in some regions have caused concerns about the possibility of selection of viral resistant populations. Although this phenomenon has not been detected in the field yet, a laboratory A. gemmatalis populations has been selected for high resistance to the AgMNPV. It is known that the midgut is considered one of the most important barriers against viral invasion, before systemic infections can be caused in various tissues if the virus succeeds in reaching the host larva hemocele. Our work aimed to verify whether or not the AgMNPV invades and infects the midgut cells of resistant larvae, comparing the ultrastructure of the midgut epithelial cells from the susceptible (SL) and resistant (RL) A. gemmatalis infected larvae. The susceptible and resistant strains of A. gemmatalis were reared on artificial diet, under laboratory-controlled conditions at Embrapa Soja, Londrina-PR, Brazil. The AgMNPV used as inoculum was incorporated into the insect diet at 60,000 occlusion bodies/ml of diet. The midguts were collected up to 120h post infection, processed and analyzed under transmission electron microscopy. The columnar cell were the most affected ones among the different midgut epithelial cells. In the SL, these cells showed many morphological signs of cellular damage from 24 hours after infection, mainly at the proximal midgut region. However, in RL these cells exhibited minor morphological damage up to 120 hours. Polyhedra were visualized in the midgut of both SL an RL from 96 hours of infection, but the amount of viral structures were always higher in SL, affecting the midgut epithelial and tracheal cells, as well as attached hemocytes (mainly plasmatocytes and granulocytes). Our results showed that the AgMNPV, in fact, invaded RL cells. However, the virus did not affect these insects as they did with the susceptible ones, allowing development and survival of resistant larvae. The mechanism interfering with the virus infection progress in midgut cells of RL it still unknown. This work has been supported by FAPESP and PRONEX (MCT/Finep/CNPq).
650 $aSoybeans
650 $aBaculovirus
650 $aControle Biológico
650 $aPraga de Planta
650 $aResistência
650 $aSoja
700 1 $aFALLEIROS, A. M. F.
700 1 $aMOSCARDI, F.
700 1 $aGREGÓRIO, E. A.
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Registro original: |
Embrapa Soja (CNPSO) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
15/11/2021 |
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Data da última atualização: |
10/04/2024 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
MENDES, G. S. B.; OLIVEIRA, R. P. de; BARROS, B. de A.; MAGALHAES, J. V. de; GUIMARÃES, C. T. |
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Afiliação: |
GABRIELA STEFANE BARBOSA MENDES; RAYANNE PEREIRA DE OLIVEIRA; BEATRIZ DE ALMEIDA BARROS, CNPMS; JURANDIR VIEIRA DE MAGALHAES, CNPMS; CLAUDIA TEIXEIRA GUIMARAES, CNPMS. |
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Título: |
Associação de polimorfismos no gene ZmMATE1 com a tolerância ao alumínio em genótipos de milho de ampla diversidade. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA PIBIC/CNPq, 19., 2021, Sete Lagoas. [Trabalhos apresentados]. Sete Lagoas: Embrapa Milho e Sorgo, 2021. |
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Idioma: |
Português |
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Conteúdo: |
O presente trabalho explorou a variabilidade genética de linhagens-elites e de acessos do Banco de Germoplasma de Milho da Embrapa quanto à resposta ao Al em solução nutritiva, aos polimorfismos no gene ZmMATE1 e aos níveis de sua expressão. |
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Thesagro: |
Linhagem; Variação Genética; Zea Mays. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227748/1/Associacao-polimorfismos.pdf
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Marc: |
LEADER 00966nam a2200193 a 4500
001 2136130
005 2024-04-10
008 2021 bl uuuu u00u1 u #d
100 1 $aMENDES, G. S. B.
245 $aAssociação de polimorfismos no gene ZmMATE1 com a tolerância ao alumínio em genótipos de milho de ampla diversidade.$h[electronic resource]
260 $aIn: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA PIBIC/CNPq, 19., 2021, Sete Lagoas. [Trabalhos apresentados]. Sete Lagoas: Embrapa Milho e Sorgo$c2021
520 $aO presente trabalho explorou a variabilidade genética de linhagens-elites e de acessos do Banco de Germoplasma de Milho da Embrapa quanto à resposta ao Al em solução nutritiva, aos polimorfismos no gene ZmMATE1 e aos níveis de sua expressão.
650 $aLinhagem
650 $aVariação Genética
650 $aZea Mays
700 1 $aOLIVEIRA, R. P. de
700 1 $aBARROS, B. de A.
700 1 $aMAGALHAES, J. V. de
700 1 $aGUIMARÃES, C. T.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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