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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
27/11/2023 |
Data da última atualização: |
28/11/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SUNIGA, P. A. P.; MANTOVANI, C.; SANTOS, M. G. dos; EGITO, A. A. do; VERBISCK, N. V.; SANTOS, L. R. dos; DÁVILA, A. M. R.; ZIMPEL, C. K.; ZERPA, M. C. S.; CHIEBAO, D. P.; GUIMARÃES, A. M. DE S.; NASSAR, A. F. DE C.; ARAUJO, F. R. |
Afiliação: |
PAULA ADAS PEREIRA SUNIGA, UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL; CYNTHIA MANTOVANI, CNPGC; MARIA GORETTI DOS SANTOS, CNPGC; ANDREA ALVES DO EGITO, CNPGC; NEWTON VALERIO VERBISCK, CNPGC; LENITA RAMIRES DOS SANTOS, CNPGC; ALBERTO MARTÍN RIVERA DÁVILA, INSTITUTO OSWALDO CRUZ; CRISTINA KRAEMER ZIMPEL, MICHIGAN STATE UNIVERSITY; MARIA CAROLINA SISCO ZERPA, UNIVERSIDASDE DE SÃO PAULO; DANIELA PONTES CHIEBAO, ANIMAL HEALTH RESEARCH CENTER; ANA MÁRCIA DE SÁ GUIMARÃES, UNIVERSIDADE DE SÃO PAULO; ALESSANDRA FIGUEIREDO DE CASTRO NASSAR, ANIMAL HEALTH RESEARCH CENTER; FLABIO RIBEIRO DE ARAUJO, CNPGC. |
Título: |
Glanders Diagnosis in an Asymptomatic Mare from Brazil: Insights from Serology, Microbiological Culture, Mass Spectrometry, and Genome Sequencing. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Pathogens, v. 12, e1250, 2023. |
DOI: |
https://doi.org/10.3390/pathogens12101250 |
Idioma: |
Inglês |
Conteúdo: |
ABSTRACT - This manuscript elucidates the occurrence of glanders in an asymptomatic mare from Brazil presenting positive Burkholderia mallei antibody titers. The diagnosis was established through a multi-pronged approach encompassing microbiological culture, mass spectrometry, and genome sequencing. The outbreak occurred in 2019 in Tatuí, São Paulo, Brazil, and the infected mare, despite displaying no clinical symptoms, had multiple miliary lesions in the liver, as well as intense catarrhal discharge in the trachea. Samples were collected from various organs and subjected to bacterial isolation, molecular detection, and identification. The strain was identified as B. mallei using PCR and confirmed by MALDI-TOF mass spectrometry. Whole-genome sequencing revealed a genome size of 5.51 Mb with a GC content of 65.8%, 5871 genes (including 4 rRNA and 53 tRNA genes), and 5583 coding DNA sequences (CDSs). Additionally, 227 predicted pseudogenes were detected. In silico analysis of different genomic loci that allow for differentiation with Burkholderia pseudomallei confirmed the identity of the isolate as B. mallei, in addition to the characteristic genome size. The BAC 86/19 strain was identified as lineage 3, sublineage 2, which includes other strains from Brazil, India, and Iran. The genome sequencing of this strain provides valuable information that can be used to better understand the pathogen and its epidemiology, as well as to develop diagnostic tools for glanders. |
Thesagro: |
Doença Animal; Patogenicidade. |
Thesaurus Nal: |
Animal diseases; Burkholderia mallei; Genome; Glanders; Mass spectrometry; Pathogenicity. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1158739/1/Glanders-diagnosis-Asymptomatic-Mare-2023.pdf
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Marc: |
LEADER 02616naa a2200373 a 4500 001 2158739 005 2023-11-28 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.3390/pathogens12101250$2DOI 100 1 $aSUNIGA, P. A. P. 245 $aGlanders Diagnosis in an Asymptomatic Mare from Brazil$bInsights from Serology, Microbiological Culture, Mass Spectrometry, and Genome Sequencing.$h[electronic resource] 260 $c2023 520 $aABSTRACT - This manuscript elucidates the occurrence of glanders in an asymptomatic mare from Brazil presenting positive Burkholderia mallei antibody titers. The diagnosis was established through a multi-pronged approach encompassing microbiological culture, mass spectrometry, and genome sequencing. The outbreak occurred in 2019 in Tatuí, São Paulo, Brazil, and the infected mare, despite displaying no clinical symptoms, had multiple miliary lesions in the liver, as well as intense catarrhal discharge in the trachea. Samples were collected from various organs and subjected to bacterial isolation, molecular detection, and identification. The strain was identified as B. mallei using PCR and confirmed by MALDI-TOF mass spectrometry. Whole-genome sequencing revealed a genome size of 5.51 Mb with a GC content of 65.8%, 5871 genes (including 4 rRNA and 53 tRNA genes), and 5583 coding DNA sequences (CDSs). Additionally, 227 predicted pseudogenes were detected. In silico analysis of different genomic loci that allow for differentiation with Burkholderia pseudomallei confirmed the identity of the isolate as B. mallei, in addition to the characteristic genome size. The BAC 86/19 strain was identified as lineage 3, sublineage 2, which includes other strains from Brazil, India, and Iran. The genome sequencing of this strain provides valuable information that can be used to better understand the pathogen and its epidemiology, as well as to develop diagnostic tools for glanders. 650 $aAnimal diseases 650 $aBurkholderia mallei 650 $aGenome 650 $aGlanders 650 $aMass spectrometry 650 $aPathogenicity 650 $aDoença Animal 650 $aPatogenicidade 700 1 $aMANTOVANI, C. 700 1 $aSANTOS, M. G. dos 700 1 $aEGITO, A. A. do 700 1 $aVERBISCK, N. V. 700 1 $aSANTOS, L. R. dos 700 1 $aDÁVILA, A. M. R. 700 1 $aZIMPEL, C. K. 700 1 $aZERPA, M. C. S. 700 1 $aCHIEBAO, D. P. 700 1 $aGUIMARÃES, A. M. DE S. 700 1 $aNASSAR, A. F. DE C. 700 1 $aARAUJO, F. R. 773 $tPathogens$gv. 12, e1250, 2023.
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Embrapa Gado de Corte (CNPGC) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
20/12/2010 |
Data da última atualização: |
24/09/2019 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
ASSIS, R. N. de; ARAÚJO, S. A. D. de; MOURA, G. S.; PINHEIRO, R. R.; DANTAS, T. V. M.; ANDRIOLI, A.; LIMA, F. E. S.; DIAS, R. P.; MELO, V. S. P.; MORAIS, S. M.; TEIXEIRA, M. F. S. |
Afiliação: |
Graduando Universidade Federal da Paraíba (UFPB); UFPB; UFPB; RAYMUNDO RIZALDO PINHEIRO, CNPC; Pós-graduando Universidade Federal do Ceará - UFC, Fortaleza, CE.; ALICE ANDRIOLI, CNPC; Pós-graduando Universidade Federal do Rio Grande do Sul - UFRGS; Pós-graduando Universidade Estadual do Ceará - UECe, Fortaleza, CE.; UFPB; UECe; UECe. |
Título: |
Atividade antiviral in vitro de Melia azedarach L. contra lentivírus de pequenos ruminantes. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MEDICINA VETERINÁRIA, 37., 2010, Rio de Janeiro. Alimento e biosseguranção para o Brasil e o mundo: trabalhos científicos apresentados. Rio de Janeiro: SOMERJ: SBMV, 2010. 1 f. 1 CD-ROM. |
Idioma: |
Português |
Palavras-Chave: |
Citotoxidade; Fitopaterapia. |
Thesagro: |
Antivírus; Caprino; Doença animal; Extrato vegetal; Melia azedarach; Ovino; Planta medicinal. |
Thesaurus NAL: |
Lentivirus. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01130nam a2200337 a 4500 001 1870445 005 2019-09-24 008 2010 bl uuuu u00u1 u #d 100 1 $aASSIS, R. N. de 245 $aAtividade antiviral in vitro de Melia azedarach L. contra lentivírus de pequenos ruminantes.$h[electronic resource] 260 $aIn: CONGRESSO BRASILEIRO DE MEDICINA VETERINÁRIA, 37., 2010, Rio de Janeiro. Alimento e biosseguranção para o Brasil e o mundo: trabalhos científicos apresentados. Rio de Janeiro: SOMERJ: SBMV, 2010. 1 f. 1 CD-ROM.$c2010 650 $aLentivirus 650 $aAntivírus 650 $aCaprino 650 $aDoença animal 650 $aExtrato vegetal 650 $aMelia azedarach 650 $aOvino 650 $aPlanta medicinal 653 $aCitotoxidade 653 $aFitopaterapia 700 1 $aARAÚJO, S. A. D. de 700 1 $aMOURA, G. S. 700 1 $aPINHEIRO, R. R. 700 1 $aDANTAS, T. V. M. 700 1 $aANDRIOLI, A. 700 1 $aLIMA, F. E. S. 700 1 $aDIAS, R. P. 700 1 $aMELO, V. S. P. 700 1 $aMORAIS, S. M. 700 1 $aTEIXEIRA, M. F. S.
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