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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
27/06/1995 |
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Data da última atualização: |
27/06/1995 |
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Autoria: |
BUSHBY, H. V. A. |
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Título: |
Colonization of rhizospheres and nodulation of two Vigna species by rhizobia inoculated into seed influence of soil. |
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Ano de publicação: |
1984 |
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Fonte/Imprenta: |
Soil Biol. Biochem., v.16, n. 6, p. 635-642, 1984. |
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Idioma: |
Português |
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Palavras-Chave: |
Caupi; Colonização/competição. |
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Thesagro: |
Antibiótico; Nodulação; Rhizobium. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00524naa a2200169 a 4500
001 1597845
005 1995-06-27
008 1984 bl uuuu u00u1 u #d
100 1 $aBUSHBY, H. V. A.
245 $aColonization of rhizospheres and nodulation of two Vigna species by rhizobia inoculated into seed influence of soil.
260 $c1984
650 $aAntibiótico
650 $aNodulação
650 $aRhizobium
653 $aCaupi
653 $aColonização/competição
773 $tSoil Biol. Biochem.$gv.16, n. 6, p. 635-642, 1984.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
08/02/2018 |
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Data da última atualização: |
26/10/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BARBÃO, G. A. A.; FUGITA, F. L. S.; MELO, I. S. de; MORAES, L. A. B. |
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Afiliação: |
G. A. A. BARBÃO; F. L. S. FUGITA; ITAMAR SOARES DE MELO, CNPMA; L. A. B. MORAES. |
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Título: |
Biological screening to identify antifungal agents produced by Streptomyces sp. isolated from the Caatinga Biome. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In: BRAZILIAN CONFERENCE ON NATURAL PRODUCTS, 6.; ANUAL MEETING ON MICROMOLECULAR EVOLUTION, SYSTEMATICS AND ECOLOGY, 32., 2017. Vitória. Annals... Vitória: UFES, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
Microbial secondary metabolites have been considered a promising natural source to disclose biologically active compounds, which can be applied in the pharmaceutical and agroindustrial sectors. Actinobacterias have played an important role in the historical advances of antibiotics and antifungals development1,2. Besides the wide application in the medicinal field as an antifungal agent, it can be also used as a natural alternative to inhibit the pathogenic microorganism?s growth in agriculture. On the other hand, now concerning the citrus fruits market, an inefficient fungi infection control results in a significant profit loss. This study shows the antibiosis screening assays of 196 microorganism?s crude extracts in order to identify antifungal activity against Colletotrichum gloeosporioides fungi. The Streptomyces sp. Caat 5-35 crude extract presented the highest antifungal activity (Figure 1-A). Mass spectrometry (MS) is an important dereplication process tool of these metabolites in the crude extract. It allows fast and sensitive analysis and provides valuable structural information. The active crude extract was fractionated in a preparative HPLC method and bioassays were performed in all the fractions in order to identify the active fraction. The Fr16 fraction exhibited notable antimicrobial activity by disk diffusion method assay. The chromatogram LC-MS/MS for the active fraction presented only one peak at 5.16 minutes, which corresponds to the ionic species [M+H]+ of m/z 521.2. Spectroscopic UV, NMR 1D and 2D data confirm that the active compound is the Albofungin3. MenosMicrobial secondary metabolites have been considered a promising natural source to disclose biologically active compounds, which can be applied in the pharmaceutical and agroindustrial sectors. Actinobacterias have played an important role in the historical advances of antibiotics and antifungals development1,2. Besides the wide application in the medicinal field as an antifungal agent, it can be also used as a natural alternative to inhibit the pathogenic microorganism?s growth in agriculture. On the other hand, now concerning the citrus fruits market, an inefficient fungi infection control results in a significant profit loss. This study shows the antibiosis screening assays of 196 microorganism?s crude extracts in order to identify antifungal activity against Colletotrichum gloeosporioides fungi. The Streptomyces sp. Caat 5-35 crude extract presented the highest antifungal activity (Figure 1-A). Mass spectrometry (MS) is an important dereplication process tool of these metabolites in the crude extract. It allows fast and sensitive analysis and provides valuable structural information. The active crude extract was fractionated in a preparative HPLC method and bioassays were performed in all the fractions in order to identify the active fraction. The Fr16 fraction exhibited notable antimicrobial activity by disk diffusion method assay. The chromatogram LC-MS/MS for the active fraction presented only one peak at 5.16 minutes, which corresponds to the ionic species [M+H]+ of ... Mostrar Tudo |
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Thesagro: |
Fungo. |
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Thesaurus NAL: |
Streptomyces. |
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Categoria do assunto: |
V Taxonomia de Organismos |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/217089/1/2017SP-Barbao-biological-13033.pdf
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Marc: |
LEADER 02259nam a2200169 a 4500
001 2087491
005 2020-10-26
008 2017 bl uuuu u00u1 u #d
100 1 $aBARBÃO, G. A. A.
245 $aBiological screening to identify antifungal agents produced by Streptomyces sp. isolated from the Caatinga Biome.$h[electronic resource]
260 $aIn: BRAZILIAN CONFERENCE ON NATURAL PRODUCTS, 6.; ANUAL MEETING ON MICROMOLECULAR EVOLUTION, SYSTEMATICS AND ECOLOGY, 32., 2017. Vitória. Annals... Vitória: UFES$c2017
520 $aMicrobial secondary metabolites have been considered a promising natural source to disclose biologically active compounds, which can be applied in the pharmaceutical and agroindustrial sectors. Actinobacterias have played an important role in the historical advances of antibiotics and antifungals development1,2. Besides the wide application in the medicinal field as an antifungal agent, it can be also used as a natural alternative to inhibit the pathogenic microorganism?s growth in agriculture. On the other hand, now concerning the citrus fruits market, an inefficient fungi infection control results in a significant profit loss. This study shows the antibiosis screening assays of 196 microorganism?s crude extracts in order to identify antifungal activity against Colletotrichum gloeosporioides fungi. The Streptomyces sp. Caat 5-35 crude extract presented the highest antifungal activity (Figure 1-A). Mass spectrometry (MS) is an important dereplication process tool of these metabolites in the crude extract. It allows fast and sensitive analysis and provides valuable structural information. The active crude extract was fractionated in a preparative HPLC method and bioassays were performed in all the fractions in order to identify the active fraction. The Fr16 fraction exhibited notable antimicrobial activity by disk diffusion method assay. The chromatogram LC-MS/MS for the active fraction presented only one peak at 5.16 minutes, which corresponds to the ionic species [M+H]+ of m/z 521.2. Spectroscopic UV, NMR 1D and 2D data confirm that the active compound is the Albofungin3.
650 $aStreptomyces
650 $aFungo
700 1 $aFUGITA, F. L. S.
700 1 $aMELO, I. S. de
700 1 $aMORAES, L. A. B.
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Embrapa Meio Ambiente (CNPMA) |
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