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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
28/01/2021 |
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Data da última atualização: |
10/06/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARAÚJO, F. C. B. das; CUNHA, E. F. M.; RODRIGUES, S. de M.; BONFIM, K.; TAVARES, E. J. M.; MOURA, M. F.; SILVA, G. F. da. |
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Afiliação: |
Francisca Chagas Bezerra das Araújo, UFRA; ELISA FERREIRA MOURA CUNHA, CPATU; SIMONE DE MIRANDA RODRIGUES, CPATU; KENNY BONFIM DE ARRUDA CARVALHO, Cenargen; ERALDO JOSE MADUREIRA TAVARES, CPATSA; Mônika Fecury Moura, CPATU; GILVAN FERREIRA DA SILVA, CPAA. |
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Título: |
Identification and validation of SNPs in the phytoene synthase 2 (psy2) gene associated with yellow color of the root in cassava (Manihot esculenta Crantz) accessions of the Brazilian Amazon. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Genetic Resources and Crop Evolution, v. 68, p. 1809-1824, 2021. |
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DOI: |
https://doi.org/10.1007/s10722-020-01097-x |
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Idioma: |
Inglês |
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Conteúdo: |
The biofortification of cassava (Manihot esculenta Crantz) roots is one of the actual goals of genetic breeding, with the development of roots richer in carotenoids. In order to evaluate the natural genetic variation of cassava, this study analyzed 78 accessions from the Brazilian Amazon to verify the presence of single nucleotide polymorphism (SNP) molecular markers in the phytoene synthase 2 (psy2) gene associated with root color. From the sequencing of 294 bp containing part of the intron 2 and the exon 3 of the psy2 gene, three SNPs located in the coding region and the existence of eight haplotypes were identified. The c.1434G > C (SNP1) and c.1485C > A (SNP3) variations were found in yellow and cream root accessions and nucleotide polymorphism resulted in amino acid substitution. SNP2 (c.1462T > C) was identified in accessions with white roots and its allelic variation did not result in amino acid substitution. Four haplotypes that presented SNP3 were widely distributed in the states of Pará, Amapá, Rondônia and Amazonas. All accessions of yellow or cream root that presented the SNP3 were in heterozygosity, confirming the dominance of the SNP, except for one accession, that could be used in genetic breeding. The effect of mutations on the structure and function of PSY2 was in silico evaluated by molecular dynamics and it was indicated that the aminoacid substitutions affected the active site of the protein depending on the distance. The results were compatible with the phenotype detected experimentally. MenosThe biofortification of cassava (Manihot esculenta Crantz) roots is one of the actual goals of genetic breeding, with the development of roots richer in carotenoids. In order to evaluate the natural genetic variation of cassava, this study analyzed 78 accessions from the Brazilian Amazon to verify the presence of single nucleotide polymorphism (SNP) molecular markers in the phytoene synthase 2 (psy2) gene associated with root color. From the sequencing of 294 bp containing part of the intron 2 and the exon 3 of the psy2 gene, three SNPs located in the coding region and the existence of eight haplotypes were identified. The c.1434G > C (SNP1) and c.1485C > A (SNP3) variations were found in yellow and cream root accessions and nucleotide polymorphism resulted in amino acid substitution. SNP2 (c.1462T > C) was identified in accessions with white roots and its allelic variation did not result in amino acid substitution. Four haplotypes that presented SNP3 were widely distributed in the states of Pará, Amapá, Rondônia and Amazonas. All accessions of yellow or cream root that presented the SNP3 were in heterozygosity, confirming the dominance of the SNP, except for one accession, that could be used in genetic breeding. The effect of mutations on the structure and function of PSY2 was in silico evaluated by molecular dynamics and it was indicated that the aminoacid substitutions affected the active site of the protein depending on the distance. T... Mostrar Tudo |
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Palavras-Chave: |
Biofortificação; Raíz rica em carotenoide. |
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Thesagro: |
Carotenóide; Mandioca; Manihot Esculenta; Polimorfismo; Proteína; Raiz Alimentícia; Variação Genética. |
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Thesaurus Nal: |
Carotenoids; Cassava; Haplotypes; Polymorphism. |
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Categoria do assunto: |
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G Melhoramento Genético |
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Marc: |
LEADER 02740naa a2200361 a 4500
001 2129648
005 2021-06-10
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s10722-020-01097-x$2DOI
100 1 $aARAÚJO, F. C. B. das
245 $aIdentification and validation of SNPs in the phytoene synthase 2 (psy2) gene associated with yellow color of the root in cassava (Manihot esculenta Crantz) accessions of the Brazilian Amazon.$h[electronic resource]
260 $c2021
520 $aThe biofortification of cassava (Manihot esculenta Crantz) roots is one of the actual goals of genetic breeding, with the development of roots richer in carotenoids. In order to evaluate the natural genetic variation of cassava, this study analyzed 78 accessions from the Brazilian Amazon to verify the presence of single nucleotide polymorphism (SNP) molecular markers in the phytoene synthase 2 (psy2) gene associated with root color. From the sequencing of 294 bp containing part of the intron 2 and the exon 3 of the psy2 gene, three SNPs located in the coding region and the existence of eight haplotypes were identified. The c.1434G > C (SNP1) and c.1485C > A (SNP3) variations were found in yellow and cream root accessions and nucleotide polymorphism resulted in amino acid substitution. SNP2 (c.1462T > C) was identified in accessions with white roots and its allelic variation did not result in amino acid substitution. Four haplotypes that presented SNP3 were widely distributed in the states of Pará, Amapá, Rondônia and Amazonas. All accessions of yellow or cream root that presented the SNP3 were in heterozygosity, confirming the dominance of the SNP, except for one accession, that could be used in genetic breeding. The effect of mutations on the structure and function of PSY2 was in silico evaluated by molecular dynamics and it was indicated that the aminoacid substitutions affected the active site of the protein depending on the distance. The results were compatible with the phenotype detected experimentally.
650 $aCarotenoids
650 $aCassava
650 $aHaplotypes
650 $aPolymorphism
650 $aCarotenóide
650 $aMandioca
650 $aManihot Esculenta
650 $aPolimorfismo
650 $aProteína
650 $aRaiz Alimentícia
650 $aVariação Genética
653 $aBiofortificação
653 $aRaíz rica em carotenoide
700 1 $aCUNHA, E. F. M.
700 1 $aRODRIGUES, S. de M.
700 1 $aBONFIM, K.
700 1 $aTAVARES, E. J. M.
700 1 $aMOURA, M. F.
700 1 $aSILVA, G. F. da
773 $tGenetic Resources and Crop Evolution$gv. 68, p. 1809-1824, 2021.
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja; Embrapa Trigo. |
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Data corrente: |
14/12/2022 |
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Data da última atualização: |
14/12/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MILIOLI, A. S.; MEIRA, D.; PANHO, M. C.; MANDELLA, L. A.; WOYANN, L. G.; TODESCHINI, M. H.; ZDZIARSKI, A. D.; CAMPAGNOLLI, O. R.; MENEGAZZI, C. P.; COLONELLI, L. L.; FERNANDES, R. A. T.; MELO, C. L. P. de; OLIVEIRA, M. F. de; BERTAGNOLLI, P. F.; ARIAS, C. A. A.; GIASSON, N. F.; MATSUMOTO, M. N.; QUIROGA, M; SILVA, R. R.; BERTAN, I.; CAPELIN, M. A.; MATEI, G.; BENIN, G. |
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Afiliação: |
ANDERSON SIMIONATO MILIOLI, Universidade Tecnológica Federal do Paraná/Pato Branco; DANIELA MEIRA, Universidade Tecnológica Federal do Paraná/Pato Branco; MAIARA CECÍLIA PANHO, Universidade Tecnológica Federal do Paraná/Pato Branco; LAURA ALEXANDRA MADELLA, Universidade Tecnológica Federal do Paraná/Pato Branco; LEOMAR GUILHERME WOYANN, Universidade Tecnológica Federal do Paraná/Pato Branco; MATHEUS HENRIQUE TODESCHINI, TMG Tropical Melhoramento e Genética; ANDREI DANIEL ZDZIARSKI, GDM Seeds; OTÁVIO RAMOS CAMPAGNOLLI, Universidade Tecnológica Federal do Paraná/Pato Branco; CAROLINE PATRÍCIA MENEGAZZI, Universidade Tecnológica Federal do Paraná/Pato Branco; LUCAS LEITE COLONELLI, Universidade Tecnológica Federal do Paraná/Pato Branco; ROGÊ AFONSO TOLENTINO FERNANDES, Universidade Tecnológica Federal do Paraná/Pato Branco; CARLOS LASARO PEREIRA DE MELO, CNPSO; MARCELO FERNANDES DE OLIVEIRA, CNPSO; PAULO FERNANDO BERTAGNOLLI, CNPT; CARLOS ALBERTO ARRABAL ARIAS, CNPSO; NIZIO FERNANDO GIASSON, GDM Seeds; MARCOS NORIO MATSUMOTO, GDM Seeds; MARCOS QUIROGA, GDM Seeds; RAPHAEL ROSSI SILVA, TMG Tropical Melhoramento e Genética; IVANDRO BERTAN, Syngenta; MARCIO ANDREI CAPELIN, Syngenta; GILVANI MATEI, Syngenta; GIOVANI BENIN, Universidade Tecnológica Federal do Paraná/Pato Branco. |
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Título: |
Genetic improvement of soybeans in Brazil: South and Midwest regions |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Crop Science, v. 62, n. 6, p. 2276-2293, 2022. |
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DOI: |
https://doi.org/10.1002/csc2.20820 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Soybean [Glycine max (L.) Merril] is one of the main crops produced worldwide, and on-farm yields have increased considerably in the last decades in Brazil. We evaluated the genetic gain for agronomic, phenological, and end-use quality traits in 29 cultivars in the South Region, and in 38 cultivars in the Midwest Region in Brazil, released from 1966 to 2011. Field trials were conducted in Macroregions 1, 2, and 4, in 2016?2017, 2017?2018, and 2018?2019 crop seasons. The best linear unbiased predictors (BLUP) of the cultivars were obtained for each trait using a linear model. The BLUPs were regressed with the year of release using linear and quadratic regression models. The rates of genetic gain for seed yield ranged from 11.98 to 15.31 kg ha?1 yr?1 (0.33 to 0.42% yr?1) in the South Region, and from 13.58 to 21.84 kg ha?1 yr?1 (0.47 to 0.77% yr?1) in the Midwest Region. New cultivars presented taller plants and more seed oil content, oil and protein yield, and lower seed weight, days to flowering, days to maturity, and seed protein content than old cultivars in the South Region, although with differences between the Macroregions. In the Midwest Region, new cultivars showed higher seed oil content, oil and protein yield, and lower bottom pod height and seed protein content than old cultivars. Our results showed that breeding programs have been efficient to improve soybean yield and other traits across the years, without yield plateaus in sight. |
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Thesagro: |
Melhoramento Genético Vegetal; Produtividade; Soja. |
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Thesaurus NAL: |
Plant genetics; Soybeans. |
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Categoria do assunto: |
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X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1149696/1/Genetic-Improvement-of-Soybeans-in-Brazil-Final-Published-Version.pdf
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Marc: |
LEADER 02735naa a2200457 a 4500
001 2149696
005 2022-12-14
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1002/csc2.20820$2DOI
100 1 $aMILIOLI, A. S.
245 $aGenetic improvement of soybeans in Brazil$bSouth and Midwest regions$h[electronic resource]
260 $c2022
520 $aAbstract: Soybean [Glycine max (L.) Merril] is one of the main crops produced worldwide, and on-farm yields have increased considerably in the last decades in Brazil. We evaluated the genetic gain for agronomic, phenological, and end-use quality traits in 29 cultivars in the South Region, and in 38 cultivars in the Midwest Region in Brazil, released from 1966 to 2011. Field trials were conducted in Macroregions 1, 2, and 4, in 2016?2017, 2017?2018, and 2018?2019 crop seasons. The best linear unbiased predictors (BLUP) of the cultivars were obtained for each trait using a linear model. The BLUPs were regressed with the year of release using linear and quadratic regression models. The rates of genetic gain for seed yield ranged from 11.98 to 15.31 kg ha?1 yr?1 (0.33 to 0.42% yr?1) in the South Region, and from 13.58 to 21.84 kg ha?1 yr?1 (0.47 to 0.77% yr?1) in the Midwest Region. New cultivars presented taller plants and more seed oil content, oil and protein yield, and lower seed weight, days to flowering, days to maturity, and seed protein content than old cultivars in the South Region, although with differences between the Macroregions. In the Midwest Region, new cultivars showed higher seed oil content, oil and protein yield, and lower bottom pod height and seed protein content than old cultivars. Our results showed that breeding programs have been efficient to improve soybean yield and other traits across the years, without yield plateaus in sight.
650 $aPlant genetics
650 $aSoybeans
650 $aMelhoramento Genético Vegetal
650 $aProdutividade
650 $aSoja
700 1 $aMEIRA, D.
700 1 $aPANHO, M. C.
700 1 $aMANDELLA, L. A.
700 1 $aWOYANN, L. G.
700 1 $aTODESCHINI, M. H.
700 1 $aZDZIARSKI, A. D.
700 1 $aCAMPAGNOLLI, O. R.
700 1 $aMENEGAZZI, C. P.
700 1 $aCOLONELLI, L. L.
700 1 $aFERNANDES, R. A. T.
700 1 $aMELO, C. L. P. de
700 1 $aOLIVEIRA, M. F. de
700 1 $aBERTAGNOLLI, P. F.
700 1 $aARIAS, C. A. A.
700 1 $aGIASSON, N. F.
700 1 $aMATSUMOTO, M. N.
700 1 $aQUIROGA, M
700 1 $aSILVA, R. R.
700 1 $aBERTAN, I.
700 1 $aCAPELIN, M. A.
700 1 $aMATEI, G.
700 1 $aBENIN, G.
773 $tCrop Science$gv. 62, n. 6, p. 2276-2293, 2022.
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