Data/hora: 10/06/2024 09:17:28

Biblioteca(s): | Embrapa Meio Ambiente.
Data corrente: | 15/10/2020
Data da última atualização: | 15/10/2020
Autoria: | REZENDE, L. C. de; CARVALHO, A. L. de A.; COSTA, L. B.; HALFELD-VIEIRA, B. de A.; SILVA, L. G.; PINTO. Z. V.; MORANDI, M. A. B.; MEDEIROS, F. H. V. de; MASCARIN, G. M.; BETTIOL, W.
Afiliação: | LARISSA CASTRO DE REZANDE, UFLA; ANDRÉ LUIZ DE ANDRADE CARVALHO, UFLA; LÚCIO BERTOLDO COSTA, FCA-UNESP; BERNARDO DE ALMEIDA HALFELD VIEIRA, CNPMA; LUCAS GUEDES SILVA, FCA-UNESP; ZAYAME VEGETTE PINTO, FCA-UNESP; MARCELO AUGUSTO BOECHAT MORANDI, CNPMA; FLÁVIO HENRIQUE VASCONCELOS DE MEDEIROS, UFLA; GABRIEL MOURA MASCARIN, CNPMA; WAGNER BETTIOL, CNPMA.
Título: | Optimizing mass production of Trichoderma asperelloides by submerged liquid fermentation and its antagonism against Sclerotinia sclerotiorum.
Ano de publicação: | 2020
Fonte/Imprenta: | World Journal of Microbiology & Biotechnology, v. 36, n. 8, article 113, 2020.
ISSN: | 1573-0972
DOI: | https://doi.org/10.1007/s11274-020-02882-7
Idioma: | Inglês
Conteúdo: | Abstract: Commercial products based on Trichoderma are obtained mainly from solid-state fermentation. Submerged liquid fermentation is the most appropriate method compared to the solid medium for large-scale production of Trichoderma spp. The present study aimed to optimize the combination of key variables that influence the liquid fermentation process of Trichoderma asperelloides LQC-96 for conidial production coupled with its efficiency in the control of Sclerotinia sclerotiorum. In addition, we verified whether the optimized culture conditions can be used for the conidial production of Trichoderma erinaceum T-12 and T-18 and Trichoderma harzianum T-15. Fermentation studies were performed in shake flasks following a planned experimental design to reduce the number of tests and consumable costs. The effect of temperature, pH, photoperiod, carbon:nitrogen ratio and water activity on conidial production were assessed, which of pH was the only meaningful factor contributing to increased conidial production of T. asperelloides LQC-96. From the five variables studied initially, pH and C:N ratio were further used in the second design (rotational central composite design-RCCD). Hence, the best conditions for the production of T. asperelloides LQC-96 conidia by liquid fermentation consisted of initial pH of 3.5, C:N ratio of 200:1 at 30 °C, without glycerol, and under 24 h photoperiod. The highest conidial concentration was observed after seven days of fermentation. Under these optimal conditions, T. erinaceum T-12 and T-18, and T. harzianum T-15 were also cultivated, but only LQC-96 efficiently parasitized S. sclerotiorum, precluding sclerotium myceliogenic germination. Our findings propose optimal fermentation conditions that maximize conidial production of T. asperelloides as a potential biofungicide against S. sclerotiorum.
Palavras-Chave: | Shite mold; Submerged spores.
Thesagro: | Fermentação; Fungo Para Controle Biológico; Trichoderma.
Thesaurus Nal: | Biological control; Biological control agents; Bioreactors; Experimental design; Mass rearing; Molds (fungi); Solid state fermentation.
Categoria do assunto: | H Saúde e Patologia
Registro original: | Embrapa Meio Ambiente (CNPMA)
Exemplares: | CNPMA - (16828-1) - UPC - AP - DD -  -  -  - DIS