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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
03/09/2021 |
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Data da última atualização: |
03/09/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PACHECO, T. F.; MACHADO, B. R. C.; MORAIS JÚNIOR, W. G. de; ALMEIDA, J. R. M. de; GONCALVES, S. B. |
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Afiliação: |
THALYTA FRAGA PACHECO, CNPAE; BRENO R. C. MACHADO; WILSON G. DE MORAIS JÚNIOR, Instituto Politécnico do Porto; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE; SILVIA BELEM GONCALVES, CNPAE. |
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Título: |
Enhanced tolerance of Spathaspora passalidarum to sugarcane bagasse hydrolysate for ethanol production from xylose. |
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Complemento do título: |
. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Applied Biochemistry and Biotechnology, v. 193, n. 7, p. 2182-2197, July 2021. |
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Descrição Física: |
PDF: il. |
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DOI: |
https://doi.org/10.1007/s12010-021-03544-6 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: During the pretreatment and hydrolysis of lignocellulosic biomass to obtain a hydrolysate rich in fermentable sugars, furaldehydes (furfural and hydroxymethylfurfural), phenolic compounds, and organic acids are formed and released. These compounds inhibit yeast metabolism, reducing fermentation yields and productivity. This study initially confirmed the ability of Spathaspora passalidarum to ferment xylose and demonstrated its sensibility to the inhibitors present in the hemicellulosic sugarcane bagasse hydrolysate. Then, an adaptive laboratory evolution, with progressive increments of hydrolysate concentration, was employed to select a strain more resistant to hydrolysate inhibitors. Afterward, a central composite design was performed to maximize ethanol production using hydrolysate as substrate. At optimized conditions (initial cell concentration of 30 g/L), S. passalidarum was able to produce 19.4 g/L of ethanol with productivity, yield, and xylose consumption rate of 0.8 g/L.h and 0.4 g/g, respectively, in a sugarcane bagasse hemicellulosic hydrolysate. A kinetic model was developed to describe the inhibition of fermentation by substrate and product. The values obtained for substrate saturation and inhibition constant were Ks = 120.4 g/L and Ki = 1293.4 g/L. Ethanol concentration that stops cell growth was 30.1 g/L. There was an agreement between simulated and experimental results, with a residual standard deviation lower than 6%. |
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Palavras-Chave: |
Spathaspora passalidarum. |
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Thesagro: |
Biomassa; Cana de Açúcar; Hidrolise. |
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Thesaurus Nal: |
Biomass; Evolutionary adaptation; Hydrolysates; Lignocellulose; Sugarcane; Xylose. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02441naa a2200313 a 4500
001 2134053
005 2021-09-03
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s12010-021-03544-6$2DOI
100 1 $aPACHECO, T. F.
245 $aEnhanced tolerance of Spathaspora passalidarum to sugarcane bagasse hydrolysate for ethanol production from xylose.$h[electronic resource]
260 $c2021
300 $cPDF: il.
520 $aAbstract: During the pretreatment and hydrolysis of lignocellulosic biomass to obtain a hydrolysate rich in fermentable sugars, furaldehydes (furfural and hydroxymethylfurfural), phenolic compounds, and organic acids are formed and released. These compounds inhibit yeast metabolism, reducing fermentation yields and productivity. This study initially confirmed the ability of Spathaspora passalidarum to ferment xylose and demonstrated its sensibility to the inhibitors present in the hemicellulosic sugarcane bagasse hydrolysate. Then, an adaptive laboratory evolution, with progressive increments of hydrolysate concentration, was employed to select a strain more resistant to hydrolysate inhibitors. Afterward, a central composite design was performed to maximize ethanol production using hydrolysate as substrate. At optimized conditions (initial cell concentration of 30 g/L), S. passalidarum was able to produce 19.4 g/L of ethanol with productivity, yield, and xylose consumption rate of 0.8 g/L.h and 0.4 g/g, respectively, in a sugarcane bagasse hemicellulosic hydrolysate. A kinetic model was developed to describe the inhibition of fermentation by substrate and product. The values obtained for substrate saturation and inhibition constant were Ks = 120.4 g/L and Ki = 1293.4 g/L. Ethanol concentration that stops cell growth was 30.1 g/L. There was an agreement between simulated and experimental results, with a residual standard deviation lower than 6%.
650 $aBiomass
650 $aEvolutionary adaptation
650 $aHydrolysates
650 $aLignocellulose
650 $aSugarcane
650 $aXylose
650 $aBiomassa
650 $aCana de Açúcar
650 $aHidrolise
653 $aSpathaspora passalidarum
700 1 $aMACHADO, B. R. C.
700 1 $aMORAIS JÚNIOR, W. G. de
700 1 $aALMEIDA, J. R. M. de
700 1 $aGONCALVES, S. B.
773 $tApplied Biochemistry and Biotechnology$gv. 193, n. 7, p. 2182-2197, July 2021.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
08/01/2016 |
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Data da última atualização: |
03/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
CAMPOS, R. V.; COBUCI, J. A.; KERN, E. L.; COSTA, C. N.; McMANUS, C. M. |
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Afiliação: |
Rafael Viegas Campos; Jaime Araujo Cobuci; Elisandra Lurdes Kern; CLAUDIO NAPOLIS COSTA, CNPGL; Concepta Margaret McManus. |
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Título: |
Genetic parameters for linear type traits and milk, fat, and protein production in holstein cows in Brazil. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Asian-Australasian Journal of Animal Sciences, v. 28, n. 4, p. 476-484, 2015. |
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Idioma: |
Português |
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Conteúdo: |
The objective of this study was to estimate genetic and phenotypic parameters for linear type traits, as well as milk yield (MY), fat yield (FY) and protein yield (PY) in 18,831 Holstein cows reared in 495 herds in Brazil. Restricted maximum likelihood with a bivariate model was used for estimation genetic parameters, including fixed effects of herd-year of classification, period of classification, classifier and stage of lactation for linear type traits and herd-year of calving, season of calving and lactation order effects for production traits. The age of cow at calving was fitted as a covariate (with linear and quadratic terms), common to both models. Heritability estimates varied from 0.09 to 0.38 for linear type traits and from 0.17 to 0.24 for production traits, indicating sufficient genetic variability to achieve genetic gain through selection. In general, estimates of genetic correlations between type and production traits were low, except for udder texture and angularity that showed positive genetic correlations (>0.29) with MY, FY, and PY. Udder depth had the highest negative genetic correlation (-0.30) with production traits. Selection for final score, commonly used by farmers as a practical selection tool to improve type traits, does not lead to significant improvements in production traits, thus the use of selection indices that consider both sets of traits (production and type) seems to be the most adequate to carry out genetic selection of animals in the Brazilian herd. MenosThe objective of this study was to estimate genetic and phenotypic parameters for linear type traits, as well as milk yield (MY), fat yield (FY) and protein yield (PY) in 18,831 Holstein cows reared in 495 herds in Brazil. Restricted maximum likelihood with a bivariate model was used for estimation genetic parameters, including fixed effects of herd-year of classification, period of classification, classifier and stage of lactation for linear type traits and herd-year of calving, season of calving and lactation order effects for production traits. The age of cow at calving was fitted as a covariate (with linear and quadratic terms), common to both models. Heritability estimates varied from 0.09 to 0.38 for linear type traits and from 0.17 to 0.24 for production traits, indicating sufficient genetic variability to achieve genetic gain through selection. In general, estimates of genetic correlations between type and production traits were low, except for udder texture and angularity that showed positive genetic correlations (>0.29) with MY, FY, and PY. Udder depth had the highest negative genetic correlation (-0.30) with production traits. Selection for final score, commonly used by farmers as a practical selection tool to improve type traits, does not lead to significant improvements in production traits, thus the use of selection indices that consider both sets of traits (production and type) seems to be the most adequate to carry out genetic selection of animals in the Braz... Mostrar Tudo |
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Palavras-Chave: |
Functional Trait; Selection. |
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Thesaurus NAL: |
dairy cattle; genetic correlation; heritability. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/136843/1/Cnpgl-2015-AJAS-Genetic.pdf
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Marc: |
LEADER 02214naa a2200229 a 4500
001 2033399
005 2024-02-03
008 2015 bl uuuu u00u1 u #d
100 1 $aCAMPOS, R. V.
245 $aGenetic parameters for linear type traits and milk, fat, and protein production in holstein cows in Brazil.$h[electronic resource]
260 $c2015
520 $aThe objective of this study was to estimate genetic and phenotypic parameters for linear type traits, as well as milk yield (MY), fat yield (FY) and protein yield (PY) in 18,831 Holstein cows reared in 495 herds in Brazil. Restricted maximum likelihood with a bivariate model was used for estimation genetic parameters, including fixed effects of herd-year of classification, period of classification, classifier and stage of lactation for linear type traits and herd-year of calving, season of calving and lactation order effects for production traits. The age of cow at calving was fitted as a covariate (with linear and quadratic terms), common to both models. Heritability estimates varied from 0.09 to 0.38 for linear type traits and from 0.17 to 0.24 for production traits, indicating sufficient genetic variability to achieve genetic gain through selection. In general, estimates of genetic correlations between type and production traits were low, except for udder texture and angularity that showed positive genetic correlations (>0.29) with MY, FY, and PY. Udder depth had the highest negative genetic correlation (-0.30) with production traits. Selection for final score, commonly used by farmers as a practical selection tool to improve type traits, does not lead to significant improvements in production traits, thus the use of selection indices that consider both sets of traits (production and type) seems to be the most adequate to carry out genetic selection of animals in the Brazilian herd.
650 $adairy cattle
650 $agenetic correlation
650 $aheritability
653 $aFunctional Trait
653 $aSelection
700 1 $aCOBUCI, J. A.
700 1 $aKERN, E. L.
700 1 $aCOSTA, C. N.
700 1 $aMcMANUS, C. M.
773 $tAsian-Australasian Journal of Animal Sciences$gv. 28, n. 4, p. 476-484, 2015.
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Embrapa Gado de Leite (CNPGL) |
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