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Biblioteca(s): |
Embrapa Trigo. |
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Data corrente: |
17/02/2021 |
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Data da última atualização: |
17/02/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
POLONI, N. M.; CARVALHO, G.; VICENTINI, S. N. C.; DORIGAN, A. F.; MACIEL, J. L. N.; MCDONALD, B. A.; MOREIRA, S. I.; HAWKINS, N.; FRAAIJE, B. A.; KELLY, D. E.; KELLY, S. L.; CERESINI, P. C. |
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Afiliação: |
NADIA MARIA POLONI, Department of Crop Protecion, Agricultural Engineering, and Soil (Departamento de Fitossanidade, Engenharia Rural e Solos), São PAulo State University, UNESP; GISELLE CARVALHO, Department of Crop Protecion, Agricultural Engineering, and Soil (Departamento de Fitossanidade, Engenharia Rural e Solos), São PAulo State University, UNESP; SAMARA NUNES CAMPOS VICENTINI, Department of Crop Protecion, Agricultural Engineering, and Soil (Departamento de Fitossanidade, Engenharia Rural e Solos), São PAulo State University, UNESP; ADRIANO FRANCIS DORIGAN, 1Department of Crop Protection, Agricultural Engineering, and Soil (Departamento de Fitossanidade, Engenharia Rural e Solos), São Paulo State University , UNESP, Ilha Solteira, São Paulo, Brazil; JOAO LEODATO NUNES MACIEL, CNPT; BRUCE A. MCDONALD, Plant Pathology Group, Institute of Integrative Biology, ETH Zürich, Zürich, Switzerland; SILVINO INTRA MOREIRA, 1Department of Crop Protection, Agricultural Engineering, and Soil (Departamento de Fitossanidade, Engenharia Rural e Solos), São Paulo State University , UNESP, Ilha Solteira, São Paulo, Brazil; NICHOLA HAWKINS, Biointeractions and Crop Protection, Rothamsted Research, Harpenden, UK; BART A. FRAAIJE, Biointeractions and Crop Protection, Rothamsted Research, Harpenden, UK; DIANE E. KELLY, 6Centre for Cytochrome P450 Biodiversity, Institute of Life Science, School of Medicine, Swansea University, Swansea, UK; STEVEN L. KELLY, 6Centre for Cytochrome P450 Biodiversity, Institute of Life Science, School of Medicine, Swansea University, Swansea, UK; PAULO C. CERESINI, 1Department of Crop Protection, Agricultural Engineering, and Soil (Departamento de Fitossanidade, Engenharia Rural e Solos), São Paulo State University , UNESP, Ilha Solteira, São Paulo, Brazil. |
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Título: |
Widespread distribution of resistance to triazole fungicides in brazilian populations of the wheat blast pathogen. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Plant Pathology, v. 1, p. ppa.13288, Sept. 2020. |
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DOI: |
10.1111/ppa.1328 |
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Idioma: |
Inglês |
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Conteúdo: |
Fungicides have not been effective in controlling the wheat blast disease in Brazil. An earlier analysis of 179 isolates of Pyricularia oryzae Triticum lineage (PoTl) sampled from wheat fields across six populations in central-southern Brazil during 2012 discovered a high level of resistance to strobilurin fungicides. Here we analysed azole resistance in the same strains based on EC50 measurements for tebuconazole and epoxiconazole. All six Brazilian populations of PoTl exhibited high resistance to both azoles, with in vitro EC50 values that were at least 35 to 50 times higher than the recommended field doses. We sequenced the CYP51A and CYP51B genes to determine if they were likely to play a role in the observed azole resistance. Although we found five distinct haplotypes in PoTl carrying four nonsynonymous substitutions in CYP51A, none of these substitutions were correlated with elevated EC50. CYP51B was sequenced for nine PoTl isolates, three each representing low, medium, and high tebuconazole EC50. Both PoTl CYP51A and CYP51B could complement yeast CYP51 function. All PoTl CYP51A-expressing yeast transformants were less sensitive to triazoles than the PoTl CYP51B ones. Transformants expressing PoTl CYP51A haplotype H1 carrying the R158K substitution were not more resistant than those expressing PoTl CYP51A haplotype H5, which is synonymous to haplotype H6, found in triazole-sensitive P. oryzae Oryza isolates from rice blast. Therefore, the reduced triazole sensitivity of wheat blast isolates compared to rice blast isolates appears to be associated with a non-target-site related resistance mechanism acquired after higher exposure to triazoles. MenosFungicides have not been effective in controlling the wheat blast disease in Brazil. An earlier analysis of 179 isolates of Pyricularia oryzae Triticum lineage (PoTl) sampled from wheat fields across six populations in central-southern Brazil during 2012 discovered a high level of resistance to strobilurin fungicides. Here we analysed azole resistance in the same strains based on EC50 measurements for tebuconazole and epoxiconazole. All six Brazilian populations of PoTl exhibited high resistance to both azoles, with in vitro EC50 values that were at least 35 to 50 times higher than the recommended field doses. We sequenced the CYP51A and CYP51B genes to determine if they were likely to play a role in the observed azole resistance. Although we found five distinct haplotypes in PoTl carrying four nonsynonymous substitutions in CYP51A, none of these substitutions were correlated with elevated EC50. CYP51B was sequenced for nine PoTl isolates, three each representing low, medium, and high tebuconazole EC50. Both PoTl CYP51A and CYP51B could complement yeast CYP51 function. All PoTl CYP51A-expressing yeast transformants were less sensitive to triazoles than the PoTl CYP51B ones. Transformants expressing PoTl CYP51A haplotype H1 carrying the R158K substitution were not more resistant than those expressing PoTl CYP51A haplotype H5, which is synonymous to haplotype H6, found in triazole-sensitive P. oryzae Oryza isolates from rice blast. Therefore, the reduced triazole sensitivity o... Mostrar Tudo |
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Palavras-Chave: |
CYP51A; CYP51B; Epoxiconazole; Pyricularia oryzae Oryza lineage; Pyricularia oryzae Triticum lineage. |
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Thesaurus NAL: |
Tebuconazole. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02669naa a2200337 a 4500
001 2130037
005 2021-02-17
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1111/ppa.1328$2DOI
100 1 $aPOLONI, N. M.
245 $aWidespread distribution of resistance to triazole fungicides in brazilian populations of the wheat blast pathogen.$h[electronic resource]
260 $c2020
520 $aFungicides have not been effective in controlling the wheat blast disease in Brazil. An earlier analysis of 179 isolates of Pyricularia oryzae Triticum lineage (PoTl) sampled from wheat fields across six populations in central-southern Brazil during 2012 discovered a high level of resistance to strobilurin fungicides. Here we analysed azole resistance in the same strains based on EC50 measurements for tebuconazole and epoxiconazole. All six Brazilian populations of PoTl exhibited high resistance to both azoles, with in vitro EC50 values that were at least 35 to 50 times higher than the recommended field doses. We sequenced the CYP51A and CYP51B genes to determine if they were likely to play a role in the observed azole resistance. Although we found five distinct haplotypes in PoTl carrying four nonsynonymous substitutions in CYP51A, none of these substitutions were correlated with elevated EC50. CYP51B was sequenced for nine PoTl isolates, three each representing low, medium, and high tebuconazole EC50. Both PoTl CYP51A and CYP51B could complement yeast CYP51 function. All PoTl CYP51A-expressing yeast transformants were less sensitive to triazoles than the PoTl CYP51B ones. Transformants expressing PoTl CYP51A haplotype H1 carrying the R158K substitution were not more resistant than those expressing PoTl CYP51A haplotype H5, which is synonymous to haplotype H6, found in triazole-sensitive P. oryzae Oryza isolates from rice blast. Therefore, the reduced triazole sensitivity of wheat blast isolates compared to rice blast isolates appears to be associated with a non-target-site related resistance mechanism acquired after higher exposure to triazoles.
650 $aTebuconazole
653 $aCYP51A
653 $aCYP51B
653 $aEpoxiconazole
653 $aPyricularia oryzae Oryza lineage
653 $aPyricularia oryzae Triticum lineage
700 1 $aCARVALHO, G.
700 1 $aVICENTINI, S. N. C.
700 1 $aDORIGAN, A. F.
700 1 $aMACIEL, J. L. N.
700 1 $aMCDONALD, B. A.
700 1 $aMOREIRA, S. I.
700 1 $aHAWKINS, N.
700 1 $aFRAAIJE, B. A.
700 1 $aKELLY, D. E.
700 1 $aKELLY, S. L.
700 1 $aCERESINI, P. C.
773 $tPlant Pathology$gv. 1, p. ppa.13288, Sept. 2020.
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