Data/hora: 06/06/2024 01:22:04

Biblioteca(s): | Embrapa Gado de Leite.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | B - 5
Data corrente: | 08/02/2012
Data da última atualização: | 05/02/2024
Autoria: | SANTOS, M. D. R.; VITOR, A. DE P.; CARNEIRO, J. da C.; PACIULLO, D. S. C.; MATOS, R. C.; MATOS, M. A. C.
Afiliação: | MELLINA DAMASCENO RACHID SANTOS, UFJF; ALINE DE PAULA VITOR, UFJF; JAILTON DA COSTA CARNEIRO, CNPGL; DOMINGOS SAVIO CAMPOS PACIULLO, CNPGL; RENATO CAMARGO MATOS, UFJF; MARIA AUXILIADORA COSTA MATOS, UFJF.
Título: | Use of ultrasound bath in the extraction and quantification of Ester-Linked Phenolic Acids in tropical forages.
Ano de publicação: | 2011
Fonte/Imprenta: | American Journal of Analytical Chemistry, v. 2, p. 344-351, 2011.
DOI: | http://dx.doi.org/10.4236/ajac.2011.23042
Idioma: | Inglês
Conteúdo: | A method was developed for the analysis of ester-linked phenolic acids in forage samples using extraction by an ultrasound-assisted treatment and quantification by HPLC with a UV-VIS detector. A reversed-phase C18 column was used for developing the method and the optimal condition was established with isocratic elution using acetonitrile/methanol/H3PO4 pH 2.08 (13:12.5:74.5) as the mobile phase. To reduce the time of sample processing, the extraction of ester-linked phenolic acids was studied using ultrasound bath and the results were then compared with those from an extraction usual using alkaline hydrolysis (20°C for 24 h). The method was valued through external and internal calibration. Internal calibration using o-coumaric acid as internal standard and m-coumaric acid as surrogate internal standard showed better results. The detection limits were of 0.09 and 0.04 mg●L-1 for p-coumaric and ferulic acids, respectively. The proposed method showed a good linear dynamic range (3.00 - 30.00 mg●L-1) for the analytes. The usefulness of the methodology was demonstrated by addition-recovery experiments using forage samples and values were in the 83 to 99% range. The extraction of ester-linked phenolic acids by 120 minutes of ultrasound bath was faster and more reproducible than alkaline hydrolysis (20°C for 24 h).
Palavras-Chave: | Internal Calibration; Ultrasound Bath.
Thesaurus Nal: | forage; phenolic acids.
Categoria do assunto: | F Plantas e Produtos de Origem Vegetal
Registro original: | Embrapa Gado de Leite (CNPGL)
Exemplares: | CNPGL - (18666-1) - UPC - AP - DD -  -  -  - DIS