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| 45. |  | PAULI, G.; LOPES, R. B.; ALVES, S. B.; DAMATTO JUNIOR, E. R.; MASCARIN, G. M. Falsa broca aumenta disseminação de Beauveria bassiana em populações de campo da broca-do-rizoma da bananeira. Ciência Rural, Santa Maria, v. 41, n. 11, p. 1867-1870, 2011.| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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| 51. | | MASCARIN, G. M.; DUARTE, V. da S.; BRANDÃO, M. M.; DELALIBERA JUNIOR, I. Natural occurrence of Zoophthora radicans (Entomophthorales: Entomophthoraceae) on Thaumastocoris peregrinus (Heteroptera: Thaumastocoridae), an invasive pest recently found in Brazil. Journal of Invertebrate Pathology, New York, v. 110, n. 3, p. 401-404, Jul. 2012.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 52. | | MASCARIN, G. M.; KOBORI, N. N.; JACKSON, M. A.; DUNLAP, C. A.; DELALIBERA JUNIOR, I. Nitrogen sources affect productivity, desiccation tolerance and storage stability of Beauveria bassiana blastospores. Journal of Applied Microbiology, v. 124, n. 3, p. 810-820, 2018.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 57. | | LOCATELLI, G. O.; FINKLER, C. L. L.; MASCARIN, G. M.; LOBO JUNIOR, M.; BUENO, L. A. Optimization of microesclerotia production by Trichoderma asperellum. In: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 21.; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, 12., 2017, Aracajú, SE. [Anais ...]. São Paulo: Associação Brasileira de Engenharia Química, 2017.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| Registros recuperados : 139 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
06/10/2021 |
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Data da última atualização: |
10/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
IWANICKI, N. S.; MASCARIN, G. M.; MORENO, S. G.; EILENBERG, J.; DELALIBERA JUNIOR, I. |
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Afiliação: |
NATASHA SANT´ANNA IWANICKI, ESALQ-USP; GABRIEL MOURA MASCARIN, CNPMA; SARA GIRO MORENO, ESALQ-USP; JØRGEN EILENBERG, University of Copenhagen; ITALO DELALIBERA JUNIOR, ESALQ-USP. |
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Título: |
Development of novel spray-dried and air-dried formulations of Metarhizium robertsii blastospores and their virulence. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Applied Microbiology and Biotechnology, v. 105, n. 20, p. 7913-7933, 2021. |
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ISSN: |
1432-0614 |
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DOI: |
https://doi.org/10.1007/s00253-021-11576-5 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The present research addressed spray-drying and air-drying techniques applied to Metarhizium robertsii blastospores to develop wettable powder (WP) formulations. We investigated the effect of co-formulants on blastospore viability during drying and assessed the wettability and stability of formulations in water. The effect of oxygen-moisture absorbers was studied on the shelf life of these formulations stored at 26 °C and 4 °C for up to 90 days. Additionally, we determined the virulence of the best spray-dried and air-dried formulations against the corn leafhopper Dalbulus maidis. While sucrose and skim milk played an essential role as osmoprotectants in preserving air-dried blastospores, maltodextrin, skim milk, and bentonite were crucial to attain high cell survival during spray drying. The lowest wettability time was achieved with spray-dried formulations containing less Ca-lignin, while charcoal powder amount was positively associated with formulation stability. The addition of oxygen-moisture absorbers inside sealed packages increased from threefold to fourfold the half-life times of air-dried and spray-dried formulations at both storage temperatures. However, the half-life times of all blastospore-based formulations were shorter than 3 months regardless of temperature and packaging system. Spray-dried and air-dried WP formulations were as virulent as fresh blastopores against D. maydis adults sprayed with 5 × 107 blastospores mL−1 that induced 87.8% and 70.6% mortality, respectively. These findings bring innovative advancement for M. robertsii blastospore formulation through spray-drying and underpin the importance of adding protective matrices coupled to oxygen-moisture absorbers to extend cell viability during either cold or non-refrigerated storage. MenosAbstract: The present research addressed spray-drying and air-drying techniques applied to Metarhizium robertsii blastospores to develop wettable powder (WP) formulations. We investigated the effect of co-formulants on blastospore viability during drying and assessed the wettability and stability of formulations in water. The effect of oxygen-moisture absorbers was studied on the shelf life of these formulations stored at 26 °C and 4 °C for up to 90 days. Additionally, we determined the virulence of the best spray-dried and air-dried formulations against the corn leafhopper Dalbulus maidis. While sucrose and skim milk played an essential role as osmoprotectants in preserving air-dried blastospores, maltodextrin, skim milk, and bentonite were crucial to attain high cell survival during spray drying. The lowest wettability time was achieved with spray-dried formulations containing less Ca-lignin, while charcoal powder amount was positively associated with formulation stability. The addition of oxygen-moisture absorbers inside sealed packages increased from threefold to fourfold the half-life times of air-dried and spray-dried formulations at both storage temperatures. However, the half-life times of all blastospore-based formulations were shorter than 3 months regardless of temperature and packaging system. Spray-dried and air-dried WP formulations were as virulent as fresh blastopores against D. maydis adults sprayed with 5 × 107 blastospores mL−1 that induc... Mostrar Tudo |
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Palavras-Chave: |
Convective drying. |
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Thesagro: |
Cigarrinha; Fermentação; Formulação; Fungo Entomógeno; Fungo Para Controle Biológico; Pó Molhável; Praga de Planta. |
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Thesaurus NAL: |
Biological control agents; Dalbulus maidis; Entomopathogenic fungi; Liquid state fermentation; Metarhizium robertsii; Pesticide formulations; Wettable powders. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 03008naa a2200373 a 4500
001 2135109
005 2022-06-10
008 2021 bl uuuu u00u1 u #d
022 $a1432-0614
024 7 $ahttps://doi.org/10.1007/s00253-021-11576-5$2DOI
100 1 $aIWANICKI, N. S.
245 $aDevelopment of novel spray-dried and air-dried formulations of Metarhizium robertsii blastospores and their virulence.$h[electronic resource]
260 $c2021
520 $aAbstract: The present research addressed spray-drying and air-drying techniques applied to Metarhizium robertsii blastospores to develop wettable powder (WP) formulations. We investigated the effect of co-formulants on blastospore viability during drying and assessed the wettability and stability of formulations in water. The effect of oxygen-moisture absorbers was studied on the shelf life of these formulations stored at 26 °C and 4 °C for up to 90 days. Additionally, we determined the virulence of the best spray-dried and air-dried formulations against the corn leafhopper Dalbulus maidis. While sucrose and skim milk played an essential role as osmoprotectants in preserving air-dried blastospores, maltodextrin, skim milk, and bentonite were crucial to attain high cell survival during spray drying. The lowest wettability time was achieved with spray-dried formulations containing less Ca-lignin, while charcoal powder amount was positively associated with formulation stability. The addition of oxygen-moisture absorbers inside sealed packages increased from threefold to fourfold the half-life times of air-dried and spray-dried formulations at both storage temperatures. However, the half-life times of all blastospore-based formulations were shorter than 3 months regardless of temperature and packaging system. Spray-dried and air-dried WP formulations were as virulent as fresh blastopores against D. maydis adults sprayed with 5 × 107 blastospores mL−1 that induced 87.8% and 70.6% mortality, respectively. These findings bring innovative advancement for M. robertsii blastospore formulation through spray-drying and underpin the importance of adding protective matrices coupled to oxygen-moisture absorbers to extend cell viability during either cold or non-refrigerated storage.
650 $aBiological control agents
650 $aDalbulus maidis
650 $aEntomopathogenic fungi
650 $aLiquid state fermentation
650 $aMetarhizium robertsii
650 $aPesticide formulations
650 $aWettable powders
650 $aCigarrinha
650 $aFermentação
650 $aFormulação
650 $aFungo Entomógeno
650 $aFungo Para Controle Biológico
650 $aPó Molhável
650 $aPraga de Planta
653 $aConvective drying
700 1 $aMASCARIN, G. M.
700 1 $aMORENO, S. G.
700 1 $aEILENBERG, J.
700 1 $aDELALIBERA JUNIOR, I.
773 $tApplied Microbiology and Biotechnology$gv. 105, n. 20, p. 7913-7933, 2021.
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