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Registro Completo |
Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
01/08/2018 |
Data da última atualização: |
01/08/2018 |
Autoria: |
AZEVEDO, F. A. de; MOURÃO FILHO, F. de A. A.; SCHINOR, E. H.; PAOLI, L. G. de; MENDES, B. M. J.; HARAKAVA, R.; GABRIEL, D. W.; LEE, R. F. |
Afiliação: |
Fernando Alves de Azevedo, Centro APTA Citros “Sylvio Moreira” - IAC; Francisco de Assis Alves Mourão Filho, Escola Superior de Agricultura Luiz de Queiroz - Esalq/Departamento de Produção Vegetal; Evandro Henrique Schinor, Escola Superior de Agricultura Luiz de Queiroz - Esalq/Departamento de Produção Vegetal; Luis Gustavo de Paoli, Escola Superior de Agricultura Luiz de Queiroz - Esalq/Departamento de Produção Vegetal; Beatriz Madalena Januzzi Mendes, Centro de Energia Nuclear na Agricultura - CENA/Laboratório de Biotecnologia Vegetal; Ricardo Harakava, Instituto Biológico; Dean W. Gabriel, University of Florida/Department of Plant Pathology; Richard F. Lee, USDA-ARS National Clonal Germplasm Repository for Citrus and Dates. |
Título: |
GUS gene expression driven by a citrus promoter in transgenic tobacco and 'Valencia' sweet orange. |
Ano de publicação: |
2006 |
Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 41, n. 11, p. 1623-1628, nov. 2006 |
Idioma: |
Inglês |
Notas: |
Título em português: Expressão do gene GUS controlado por promotor de citros em plantas transgênicas de tabaco e laranja 'Valência'. |
Conteúdo: |
The objective of this work was the transformation of tobacco and 'Valencia' sweet orange with the GUS gene driven by the citrus phenylalanine ammonia-lyase (PAL) gene promoter (CsPP). Transformation was accomplished by co-cultivation of tobacco and 'Valência' sweet orange explants with Agrobacterium tumefaciens containing the binary vector CsPP-GUS/2201. After plant transformation and regeneration, histochemical analyses using GUS staining revealed that CsPP promoter preferentially, but not exclusively, conferred gene expression in xylem tissues of tobacco. Weaker GUS staining was also detected throughout the petiole region in tobacco and citrus CsPP transgenic plants. |
Palavras-Chave: |
Clorose variegada do citro; Resistência varietal. |
Thesagro: |
Xylella Fastidiosa. |
Thesaurus Nal: |
Citrus variegated chlorosis. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/180683/1/GUS-gene-expression-driven-by-a-citrus.pdf
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Marc: |
LEADER 01631naa a2200265 a 4500 001 2093761 005 2018-08-01 008 2006 bl uuuu u00u1 u #d 100 1 $aAZEVEDO, F. A. de 245 $aGUS gene expression driven by a citrus promoter in transgenic tobacco and 'Valencia' sweet orange. 260 $c2006 500 $aTítulo em português: Expressão do gene GUS controlado por promotor de citros em plantas transgênicas de tabaco e laranja 'Valência'. 520 $aThe objective of this work was the transformation of tobacco and 'Valencia' sweet orange with the GUS gene driven by the citrus phenylalanine ammonia-lyase (PAL) gene promoter (CsPP). Transformation was accomplished by co-cultivation of tobacco and 'Valência' sweet orange explants with Agrobacterium tumefaciens containing the binary vector CsPP-GUS/2201. After plant transformation and regeneration, histochemical analyses using GUS staining revealed that CsPP promoter preferentially, but not exclusively, conferred gene expression in xylem tissues of tobacco. Weaker GUS staining was also detected throughout the petiole region in tobacco and citrus CsPP transgenic plants. 650 $aCitrus variegated chlorosis 650 $aXylella Fastidiosa 653 $aClorose variegada do citro 653 $aResistência varietal 700 1 $aMOURÃO FILHO, F. de A. A. 700 1 $aSCHINOR, E. H. 700 1 $aPAOLI, L. G. de 700 1 $aMENDES, B. M. J. 700 1 $aHARAKAVA, R. 700 1 $aGABRIEL, D. W. 700 1 $aLEE, R. F. 773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 41, n. 11, p. 1623-1628, nov. 2006
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Embrapa Unidades Centrais (AI-SEDE) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
02/08/2018 |
Data da última atualização: |
03/08/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
MORALES-REYES, C.; MASCARIN, G. M.; JACKSON, M. A.; HALL, D.; SANCHEZ-PENA, S. R.; ARTHURS, S. P. |
Afiliação: |
CELSO MORALES-REYES, Universidad Autónoma Agraria Antonio Narro, México; GABRIEL MOURA MASCARIN, CNPMA; MARK ALAN JACKSON, USDA-ARS; DAVID HALL, USDA-ARS; SERGIO R SANCHEZ-PENA, Universidad Autónoma Agraria Antonio Narro, México; STEVEN PAUL ARTHURS, University of Florida. |
Título: |
Comparison of aerial conidia and blastospores from two entomopathogenic fungi against Diaphorina citri (Hemiptera: Liviidae) under laboratory and greenhouse conditions. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Biocontrol Science and Technology, v. 28, n. 8, p. 737-749, 2018. |
DOI: |
https://doi.org/10.1080/09583157.2018.1487028 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: This study compared the insecticidal activity of liquid culture-produced blastospores and solid substrate-produced aerial conidia of Beauveria bassiana GHA and Isaria fumosorosea ARSEF3581 strains against Diaphorina citri adults. Insects exposed to 10(7) propagules/ml in a spray residue contact leaf bioassay died within 6 days at 25°C, with no significant differences between fungal treatments. At higher concentrations (10(8) propagules/ml), I. fumosorosea conidia killed psyllids faster compared to its blastospore formulation, i.e. 4 versus 5 days, respectively. In greenhouse tests, the same treatments applied to infested citrus plants (2 x 10(6) spores/ml) all significantly reduced the number of nymphs compared with the untreated controls over 3 weeks; however, only I. fumosorosea blastospores significantly reduced the number of F1 adult psyllids when compared with controls. Similar results were observed in the follow-up greenhouse test, where I. fumosorosea blastospores were the most effective treatment overall, reducing D. citri populations by about 60% after 21 days; by contrast, imidacloprid killed almost 100% of psyllids within a week in both tests. Fewer psyllids exhibited mycosis in the greenhouse (i.e. ~20 versus >- 87% in the laboratory). This is the first report comparing both conidial and blastospore formulations of B. bassiana and I. fumosorosea for the control of a psyllid pest. Field testing is required to determine how successful different spore formulations might be under various environmental conditions. MenosAbstract: This study compared the insecticidal activity of liquid culture-produced blastospores and solid substrate-produced aerial conidia of Beauveria bassiana GHA and Isaria fumosorosea ARSEF3581 strains against Diaphorina citri adults. Insects exposed to 10(7) propagules/ml in a spray residue contact leaf bioassay died within 6 days at 25°C, with no significant differences between fungal treatments. At higher concentrations (10(8) propagules/ml), I. fumosorosea conidia killed psyllids faster compared to its blastospore formulation, i.e. 4 versus 5 days, respectively. In greenhouse tests, the same treatments applied to infested citrus plants (2 x 10(6) spores/ml) all significantly reduced the number of nymphs compared with the untreated controls over 3 weeks; however, only I. fumosorosea blastospores significantly reduced the number of F1 adult psyllids when compared with controls. Similar results were observed in the follow-up greenhouse test, where I. fumosorosea blastospores were the most effective treatment overall, reducing D. citri populations by about 60% after 21 days; by contrast, imidacloprid killed almost 100% of psyllids within a week in both tests. Fewer psyllids exhibited mycosis in the greenhouse (i.e. ~20 versus >- 87% in the laboratory). This is the first report comparing both conidial and blastospore formulations of B. bassiana and I. fumosorosea for the control of a psyllid pest. Field testing is required to determine how successful different spore form... Mostrar Tudo |
Palavras-Chave: |
Residue bioassay. |
Thesagro: |
Beauveria Bassiana. |
Thesaurus NAL: |
Isaria fumosorosea; virulence. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02394naa a2200241 a 4500 001 2093861 005 2018-08-03 008 2018 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1080/09583157.2018.1487028$2DOI 100 1 $aMORALES-REYES, C. 245 $aComparison of aerial conidia and blastospores from two entomopathogenic fungi against Diaphorina citri (Hemiptera$bLiviidae) under laboratory and greenhouse conditions.$h[electronic resource] 260 $c2018 520 $aAbstract: This study compared the insecticidal activity of liquid culture-produced blastospores and solid substrate-produced aerial conidia of Beauveria bassiana GHA and Isaria fumosorosea ARSEF3581 strains against Diaphorina citri adults. Insects exposed to 10(7) propagules/ml in a spray residue contact leaf bioassay died within 6 days at 25°C, with no significant differences between fungal treatments. At higher concentrations (10(8) propagules/ml), I. fumosorosea conidia killed psyllids faster compared to its blastospore formulation, i.e. 4 versus 5 days, respectively. In greenhouse tests, the same treatments applied to infested citrus plants (2 x 10(6) spores/ml) all significantly reduced the number of nymphs compared with the untreated controls over 3 weeks; however, only I. fumosorosea blastospores significantly reduced the number of F1 adult psyllids when compared with controls. Similar results were observed in the follow-up greenhouse test, where I. fumosorosea blastospores were the most effective treatment overall, reducing D. citri populations by about 60% after 21 days; by contrast, imidacloprid killed almost 100% of psyllids within a week in both tests. Fewer psyllids exhibited mycosis in the greenhouse (i.e. ~20 versus >- 87% in the laboratory). This is the first report comparing both conidial and blastospore formulations of B. bassiana and I. fumosorosea for the control of a psyllid pest. Field testing is required to determine how successful different spore formulations might be under various environmental conditions. 650 $aIsaria fumosorosea 650 $avirulence 650 $aBeauveria Bassiana 653 $aResidue bioassay 700 1 $aMASCARIN, G. M. 700 1 $aJACKSON, M. A. 700 1 $aHALL, D. 700 1 $aSANCHEZ-PENA, S. R. 700 1 $aARTHURS, S. P. 773 $tBiocontrol Science and Technology$gv. 28, n. 8, p. 737-749, 2018.
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