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Registros recuperados : 44 | |
2. | | MARTINS, C. M.; MARTINS, S. C. S.; BORGES, W. L. Correção da acidez, adubação e fixação bológica. In: DOVALE, J. C.; BERTINI, C.; BORÉM, A. (Ed.). Feijão-caupi: do plantio à colheita. Viçosa, MG: Ed. UFV, 2017. Cap. 5, p. 89-112. Biblioteca(s): Embrapa Amapá. |
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7. | | MARTINS, S. C.; ASSAD, E. D.; PAVÃO, E.; LOPES-ASSAD, M. L. R. C. Inverting the carbon footprint in Brazilian agriculture: an estimate of the effects of the ABC plan. Revista Ciência, Tecnologia & Ambiente, Araras, v. 7, n. 1, p. 43-52, 2018. Biblioteca(s): Embrapa Agricultura Digital. |
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9. | | ASSAD, E. D.; MARTINS, S. C.; BELTRÃO, N. E. de M.; PINTO, H. S. Impacts of climate change on the agricultural zoning of climate risk for cotton cultivation in Brazil. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 48, n. 1, p. 1-8, jan. 2013. Biblioteca(s): Embrapa Agricultura Digital; Embrapa Unidades Centrais. |
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11. | | CHAVES, A. R. de M.; MATTA, F. M. da; MARTINS, S. C. V.; CELIN, E. F. Concentração de açúcares solúveis, amido e aminoácidos em cafeeiro em função da razão área foliar/número de frutos em diferentes posições na copa. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 8., 2013, Salvador. Sustentabilidade e inclusão Social. Brasília, DF: Embrapa Café, 2013. Biblioteca(s): Embrapa Semiárido. |
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13. | | ASSAD, E. D.; MARTINS, S. C.; CORDEIRO, L. A. M.; EVANGELISTA, B. A. Sequestro de carbono e mitigação de emissões de gases de efeito estufa pela adoção de sistemas integrados. In: BUNGENSTAB, D. J.; ALMEIDA, R. G. de; LAURA, V. A.; BALBINO, L. C.; FERREIRA, A. D. (Ed.). ILPF: inovação com integração de lavoura, pecuária e floresta. Brasília, DF: Embrapa, 2019. Cap. 11. p. 153-167. Biblioteca(s): Embrapa Agricultura Digital; Embrapa Cerrados; Embrapa Pesca e Aquicultura. |
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14. | | CHAVES, A. R. de M.; MATTA, F. M. da; CELIN, E. F.; MARTINS, S. C. V. Relação da morte de ramos com a razão área foliar por fruto, crescimento vegetativo e produção em diferentes posições da copa do cafeeiro. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 8., 2013, Salvador. Sustentabilidade e inclusão Social. Brasília, DF: Embrapa Café, 2013. Biblioteca(s): Embrapa Semiárido. |
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17. | | MARTINS, S. C.; SALGADO, P. R.; VASCONCELLOS, E. B. C.; EVANGELISTA, B. A.; PINTO, H. S.; ASSAD, E. D. Capacidade de sequestro de CO2 em pastagens produtivas no bioma Cerrado. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 33., 2011, Uberlândia. Solos nos biomas brasileiros: sustentabilidade e mudanças climáticas: anais. [Uberlândia]: SBCS: UFU, ICIAG, 2011. p. 1-4. 1 CD-ROM. Biblioteca(s): Embrapa Agricultura Digital. |
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18. | | SALGADO, P. R.; MARTINS, S. C.; VASCONCELLOS, E. B. C.; EVANGELISTA, B. A.; PINTO, H. S.; ASSAD, E. D. Capacidade de sequestro de CO2 em pastagens produtivas no bioma Mata Atlântica. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 33., 2011, Uberlândia. Solos nos biomas brasileiros: sustentabilidade e mudanças climáticas: anais. [Uberlândia]: SBCS: UFU, ICIAG, 2011. p. 1-4. 1 CD-ROM. Biblioteca(s): Embrapa Agricultura Digital. |
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19. | | SOUSA, J. B. de; PINHEIRO, M. de S.; CAVALCANTE, F. G.; MARTINS, S. C. S.; MARTINS, C. M. Caracterização de bactérias nativas de solo do Semiárido isoladas de nódulos de feijão-caupi. In: SIMPÓSIO DE MUDANÇAS CLIMÁTICAS E DESERTIFICAÇÃO NO SEMIÁRIDO BRASILEIRO, 4., 2015, Petrolina. Experiências e oportunidades para o desenvolvimento. Petrolina: Embrapa Semiárido, 2015. 1 CD-ROM. (Embrapa Semiárido. Documentos, 262). Biblioteca(s): Embrapa Semiárido. |
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20. | | FEITOZA, R. M.; SILVA, L. L. da; PINHEIRO, M. de S.; MARTINS, S. C. S.; MARTINS, C. M. Diversidade bioquímica de estirpes de rizóbio do Semiárido. In: SIMPÓSIO DE MUDANÇAS CLIMÁTICAS E DESERTIFICAÇÃO NO SEMIÁRIDO BRASILEIRO, 4., 2015, Petrolina. Experiências e oportunidades para o desenvolvimento. Petrolina: Embrapa Semiárido, 2015. 1 CD-ROM. (Embrapa Semiárido. Documentos, 262). Biblioteca(s): Embrapa Semiárido. |
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Registros recuperados : 44 | |
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
26/12/2018 |
Data da última atualização: |
24/01/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
ASCARI, I. J.; MARTINS, S. C.; CAMARGO, L. S. de A.; MENDEZ-OTERO, R.; JASMIN. |
Afiliação: |
LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
Título: |
Development of bovine embryos in vitro in coculture with murine mesenchymal stem cells and embryonic fibroblasts. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Molecular Biology Reports, v. 45, n. 6, p. 1827-1837, 2018. |
DOI: |
10.1007/s11033-018-4329-y |
Idioma: |
Inglês |
Conteúdo: |
Abstract Despite the progress on development of new culture media, in vitro-produced embryos still display lower quality when compared to the in vivo-produced counterparts. Coculture has been reconsidered as an alternative to improve embryo quality. Mesenchymal stem cells (MSC) and murine embryonic fibroblasts (MEF) have been extensively used as feeder layers due to their capacity to release growth factors. In the present study we investigated the effect of these feeder layers in oocyte maturation and/or embryo development under in vitro conditions. Oocytes were matured in control (CTRL) conditions or in coculture with MSC or MEF. In vitro fertilization and embryo culture until fourth day were performed in CTRL condition for all groups. Embryos from fourth day on were then cultured until the eighth day in CTRL or in coculture system. No significant differences for metaphase II stage and apoptosis in oocytes were found among the groups. There was also no difference among the groups when we evaluated blastocyst formation on the seventh and eighth day, with exception of a higher hatched blastocyst rate in the group maturated and cultivated in CTRL condition when compared to the group matured and cocultured with MSC. Also no difference was observed in the number of cells in the whole embryos, in the inner cell mass, in the trophoblast and at apoptotic stage on the eighth day. We conclude that coculture with MSC or MEF during maturation and/or embryo development do not enhance the in vitro production of bovine embryos. MenosAbstract Despite the progress on development of new culture media, in vitro-produced embryos still display lower quality when compared to the in vivo-produced counterparts. Coculture has been reconsidered as an alternative to improve embryo quality. Mesenchymal stem cells (MSC) and murine embryonic fibroblasts (MEF) have been extensively used as feeder layers due to their capacity to release growth factors. In the present study we investigated the effect of these feeder layers in oocyte maturation and/or embryo development under in vitro conditions. Oocytes were matured in control (CTRL) conditions or in coculture with MSC or MEF. In vitro fertilization and embryo culture until fourth day were performed in CTRL condition for all groups. Embryos from fourth day on were then cultured until the eighth day in CTRL or in coculture system. No significant differences for metaphase II stage and apoptosis in oocytes were found among the groups. There was also no difference among the groups when we evaluated blastocyst formation on the seventh and eighth day, with exception of a higher hatched blastocyst rate in the group maturated and cultivated in CTRL condition when compared to the group matured and cocultured with MSC. Also no difference was observed in the number of cells in the whole embryos, in the inner cell mass, in the trophoblast and at apoptotic stage on the eighth day. We conclude that coculture with MSC or MEF during maturation and/or embryo development do not enhance th... Mostrar Tudo |
Palavras-Chave: |
Bovine; In vitro embryonic development; Mesenchymal stem cells; Murine embryonic fibroblast. |
Thesaurus NAL: |
Coculture. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02308naa a2200241 a 4500 001 2102511 005 2023-01-24 008 2018 bl uuuu u00u1 u #d 024 7 $a10.1007/s11033-018-4329-y$2DOI 100 1 $aASCARI, I. J. 245 $aDevelopment of bovine embryos in vitro in coculture with murine mesenchymal stem cells and embryonic fibroblasts.$h[electronic resource] 260 $c2018 520 $aAbstract Despite the progress on development of new culture media, in vitro-produced embryos still display lower quality when compared to the in vivo-produced counterparts. Coculture has been reconsidered as an alternative to improve embryo quality. Mesenchymal stem cells (MSC) and murine embryonic fibroblasts (MEF) have been extensively used as feeder layers due to their capacity to release growth factors. In the present study we investigated the effect of these feeder layers in oocyte maturation and/or embryo development under in vitro conditions. Oocytes were matured in control (CTRL) conditions or in coculture with MSC or MEF. In vitro fertilization and embryo culture until fourth day were performed in CTRL condition for all groups. Embryos from fourth day on were then cultured until the eighth day in CTRL or in coculture system. No significant differences for metaphase II stage and apoptosis in oocytes were found among the groups. There was also no difference among the groups when we evaluated blastocyst formation on the seventh and eighth day, with exception of a higher hatched blastocyst rate in the group maturated and cultivated in CTRL condition when compared to the group matured and cocultured with MSC. Also no difference was observed in the number of cells in the whole embryos, in the inner cell mass, in the trophoblast and at apoptotic stage on the eighth day. We conclude that coculture with MSC or MEF during maturation and/or embryo development do not enhance the in vitro production of bovine embryos. 650 $aCoculture 653 $aBovine 653 $aIn vitro embryonic development 653 $aMesenchymal stem cells 653 $aMurine embryonic fibroblast 700 1 $aMARTINS, S. C. 700 1 $aCAMARGO, L. S. de A. 700 1 $aMENDEZ-OTERO, R. 700 1 $aJASMIN 773 $tMolecular Biology Reports$gv. 45, n. 6, p. 1827-1837, 2018.
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