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| Registros recuperados : 77 | |
| 4. |  | MARTINS, P. K.; DIAS, B. B. A.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Setaria viridis as a model plant for functional genomic studies in C4 Crops. In: KUMAR, S.; BARONE, P.; SMITH, M. Transgenic plants: methods and protocols. Berlim, Alemanha: Springer Science+Business Media, 2019. (Methods in Molecular Biology, v. 1864). p. 49-64| Biblioteca(s): Embrapa Agroenergia. |
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| 5. | | OYA, T.; MARTINS, P. K.; CASAGRANDE, E. C.; LUGLE, S. M.; NEPOMUCENO, A. L.; NEUMAIER, N.; FARIAS, J. R. B. Agronomic, physiological and molecular characteristics of drought-tolerant Brazilian soybean cultivars. In: EMBRAPA SOYBEAN/JICA-CETAPAR/INTA-EEA/JIRCAS JOINT WORKSHOP ON SOYBEAN IMPROVEMENT, PRODUCTION AND UTILIZATION IN SOUTH AMERICA, 2001, Londrina. Abstracts and program. Londrina: Embrapa Soybean: JIRCAS, 2001. p. 17. (Embrapa Soja. Documentos, 170).| Biblioteca(s): Embrapa Soja. |
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| 6. | | RIBEIRO, A. P.; MARTINS, P. K.; DIAS, B. B. A.; KOBAYASHI, A. K.; ANDRADE, A. C.; MOLINARI, H. B. C. Overexpression of the MATE gene in Setaria viridis. In: INTERNATIONAL SERTARIA GENETICS CONFERENCE, 1., 2014, Beijing, China. [Beijing]: China Agricultural Research Systems, 2014. p. 11.| Biblioteca(s): Embrapa Agroenergia. |
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| 7. | | MARTINS, P. K.; DIAS, B. B. A.; RIBEIRO, A. P.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Setaria viridis: a tool for functional gene analysis in sugarcane. In: INTERNATIONAL SERTARIA GENETICS CONFERENCE, 1., 2014, Beijing, China. [Beijing]: China Agricultural Research Systems, 2014. p. 19.| Biblioteca(s): Embrapa Agroenergia. |
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| 10. | | MAFRA, V.; MARTINS, P. K.; FRANSCISCO, C. S.; RIBEIRO-ALVES, M.; ASTUA, J. de F.; MACHADO, M. A. Candidatus Liberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotype. In; INTERNATIONAL RESEARCH CONFERENCE ON HUANGLONGBING , 3., 2013, Orlando, Flórida, USA. IRC HLB III. Proceedings. Orlando, Flórida, USA, 2013, (online).| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 12. | | MARTINS, P. K.; DIAS, B. B. A.; RIBEIRO, A. P.; SOUTO, B. de M.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Isolation, in silico analysis and transformation of Setaria viridis with a sugarcane senescence-associated promoter. In: INTERNATIONAL SERTARIA GENETICS CONFERENCE, 1., 2014, Beijing, China. [Beijing]: China Agricultural Research Systems, 2014. p. 25.| Biblioteca(s): Embrapa Agroenergia. |
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| 13. | | SOUSA, C. A. F. de; DIAS, B. B. A.; MARTINS, P. K.; MOLINARI, H. B. C.; KOBAYASHI, A. K.; SOUZA JUNIOR, M. T. Nova abordagem para a fenotipagem de plantas: conceitos, ferramentas e perspectivas. Revista Brasileira de Geografia Física, v. 8, p. 660-672, 2015.| Biblioteca(s): Embrapa Agroenergia. |
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| 14. | | DIAS, B. B. A.; KOBAYASHI, A. K.; GOMES, A. de P. G.; RODRIGUES, D. de S.; MOLINARI, H. B. C.; FAVARO, L. C. de L.; MARTINS, P. K. Manual de Biossegurança da Embrapa Agroenergia (CQB 345/12). Brasília, DF: Embrapa Agroenergia, 2015. 35 p. ( Embrapa Agroenergia. Documentos, 19).| Biblioteca(s): Embrapa Agroenergia; Embrapa Unidades Centrais. |
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| 15. | | MILORI, D. M. B. P.; BOAS, P. R. V.; VEMÂNCIO, A. L.; FERREIRA, E. J.; MARTINS, P. K.; FREITAS-ASTUA, J.; BRESOLIN, J.; CARDINALI, M. C. B. Diagnóstico de Citrus Greening (HLB) utilizando espectroscopia de fluorescência induzida por laser (LIFS). In: INAMASU, R. Y.; NAIME, J. de M.; RESENDE, A. V. de; BASSOI, L. H.; BERNARDI, A. de C. (Ed.). Agricultura de precisão: um novo olhar. São Carlos, SP: Embrapa Instrumentação, 2011. p. 78-81.| Biblioteca(s): Embrapa Instrumentação. |
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| 16. | | MARTINS, P. K.; JORDÃO, B. Q.; YAMANAKA, N.; FARIAS, J. R. B.; BENEVENTI, M. A.; BINNECK, E.; FUGANTI, R.; STOLF, R.; NEPOMUCENO, A. L. Differential gene expression and mitotic cell analysis of the drought tolerant soybean (Glycine max L. Merrill Fabales, Fabaceae) cultivar MG/BR46 (Conquista) under two water deficit induction systems. Genetics and Molecular Biology, Ribeirão Preto, v. 31, n. 2, p. 512-521, 2008.| Biblioteca(s): Embrapa Soja. |
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| 17. | | PEDROSO, J. C.; NEPOMUCENO, A. L.; SILVA, J. F. V.; CARNEIRO, N. P.; MARTINS, P. K.; BRETON, M. C.; MARIN, S. R. R.; BINNECK, E. Differential gene expression in nematode resistant soybean genotypes. Nematology, Boston, v. 4, n. 2, p. 235, 2002. Presented in Fourth International Congress of Nematology. Programme and abstracts. 8-13 June 2002, Tenbel, La Galletas, Arona, Tenerife, Canary Islands, Spain.| Biblioteca(s): Embrapa Soja. |
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| 18. | | CORREA, P. H.; MARTINS, P. K.; ARENA, G. D.; MIGUEL, M. C.; MUTTI, H. S.; MACHADO, M. A.; FREITAS-ASTUA, J. Diagnóstico molecular de huanglongbing dos citros, via qPCR, em mudas de valência sobre Swingle. In:CONGRESSO BRASILEIRO DE FRUTICULTURA, 21., 2010, Natal. Frutas: saúde, inovação e responsabilidade: anais. Natal: Sociedade Brasileira de Fruticultura, 2010. pdf 1867| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 19. | | Ribeiro, A. P.; Martins, P. K.; DIAS, B. B. A.; ANDRADE, A. C.; MAGALHAES, J. V. de; KOBAYASHI, A. K.; MOLINARI, H. B. C. MATE: a promising gene for aluminum tolerance in sugarcane. In: GERMPLASM AND BREEDING, 11.; MOLECULAR BIOLOGY ISSCT WORKSHOP, 8., 2015, Saint-Gilles Réunion Island. Pushing the frontiers of sugarcane improvement: abstract. [S.l]: Ercane, 2015.| Biblioteca(s): Embrapa Agroenergia. |
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| 20. | | MARTINS, P. K.; NAKAYAMA, T. J.; RIBEIRO, A. P.; DIAS, B. B. A.; NEPOMUCENO, A. L.; HARMON, F. G.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Setaria viridis floral-dip: a simple and rapid Agrobacterium-mediated transformation method. Biotechnology Reports, v. 6, p. 61-63, 2015.| Biblioteca(s): Embrapa Agroenergia. |
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| Registros recuperados : 77 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Instrumentação; Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
21/12/2010 |
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Data da última atualização: |
18/06/2013 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
CARDINALI, M. C. B.; VENÂNCIO, A. L.; KUBOTA, T. M. K.; MILORI, D. M. B. P.; FERREIRA, E. J.; MARTINS, P. K.; FREITAS-ASTÚA, J. |
|
Afiliação: |
DEBORA MARCONDES BASTOS P MILORI, CNPDIA; EDNALDO JOSE FERREIRA, CNPDIA. |
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Título: |
Evaluation of early diagnosis of citrus greening using laser-induced fluorescence spectroscopy. |
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Ano de publicação: |
2010 |
|
Fonte/Imprenta: |
Citrus research & technology, Cordeirópolis, v. 31, suplemento, p. 108, 2010. Proceedings of the 18 conference international organization citrus virologists, 2010, Campinas. 1CD-ROM. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Greening (HLB) is the most devastating disease that citrus plants face nowadays. The state of Sao Paulo, the largest producer of citrus in Brazil, has increasing incidences of this disease, which has no cure and makes trees unproductive over time. The strategy adopted by producers consists in elimination of symptomatic trees and spraying for vector control. However, this control is not effective because the plants have a long asymptomatic phase. This fact makes them invisible sources of inoculum. This study proposes the use of Laser-Induced Fluorescence Spectroscopy (LIFS) for early diagnosis of HLB in citrus plants. Leaves from healthy and inoculated plants with Candidatus Liberibacter asiaticus were assessed. Artificial Neural Network was used as chemometric tool for classifying spectra. Real-Time Polymerase Chain Reaction (RT-qPCR) measurements were performed to detect the presence of the bacteria. Based on the LIFS and RT-qPCR results, it is possible to determine that the chemical changes and the bacteria are unevenly distributed in the tree, and the accuracy of diagnosis increases with the number of collected leaves. Nevertheless, our results show that LIFS has great potential for early Greening diagnosis. In the third month after inoculation, around four months before the appearance of symptoms, 83% success rate was achieved. In the seventh month, LIFS has above 85% of success rate with high correlation with the RT-qPCR. |
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Palavras-Chave: |
Evento; Plant disease. |
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Thesagro: |
Bactéria; Doença de Planta; Fruta Cítrica; Greening. |
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Thesaurus NAL: |
Candidatus Liberibacter asiaticus. |
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Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/84530/1/Proci-10.00260.pdf
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Marc: |
LEADER 02362nam a2200265 a 4500
001 1881244
005 2013-06-18
008 2010 bl uuuu u00u1 u #d
100 1 $aCARDINALI, M. C. B.
245 $aEvaluation of early diagnosis of citrus greening using laser-induced fluorescence spectroscopy.
260 $aCitrus research & technology, Cordeirópolis, v. 31, suplemento, p. 108, 2010. Proceedings of the 18 conference international organization citrus virologists, 2010, Campinas. 1CD-ROM.$c2010
520 $aGreening (HLB) is the most devastating disease that citrus plants face nowadays. The state of Sao Paulo, the largest producer of citrus in Brazil, has increasing incidences of this disease, which has no cure and makes trees unproductive over time. The strategy adopted by producers consists in elimination of symptomatic trees and spraying for vector control. However, this control is not effective because the plants have a long asymptomatic phase. This fact makes them invisible sources of inoculum. This study proposes the use of Laser-Induced Fluorescence Spectroscopy (LIFS) for early diagnosis of HLB in citrus plants. Leaves from healthy and inoculated plants with Candidatus Liberibacter asiaticus were assessed. Artificial Neural Network was used as chemometric tool for classifying spectra. Real-Time Polymerase Chain Reaction (RT-qPCR) measurements were performed to detect the presence of the bacteria. Based on the LIFS and RT-qPCR results, it is possible to determine that the chemical changes and the bacteria are unevenly distributed in the tree, and the accuracy of diagnosis increases with the number of collected leaves. Nevertheless, our results show that LIFS has great potential for early Greening diagnosis. In the third month after inoculation, around four months before the appearance of symptoms, 83% success rate was achieved. In the seventh month, LIFS has above 85% of success rate with high correlation with the RT-qPCR.
650 $aCandidatus Liberibacter asiaticus
650 $aBactéria
650 $aDoença de Planta
650 $aFruta Cítrica
650 $aGreening
653 $aEvento
653 $aPlant disease
700 1 $aVENÂNCIO, A. L.
700 1 $aKUBOTA, T. M. K.
700 1 $aMILORI, D. M. B. P.
700 1 $aFERREIRA, E. J.
700 1 $aMARTINS, P. K.
700 1 $aFREITAS-ASTÚA, J.
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