| |
|
|
| Registros recuperados : 77 | |
| 4. |  | MARTINS, P. K.; DIAS, B. B. A.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Setaria viridis as a model plant for functional genomic studies in C4 Crops. In: KUMAR, S.; BARONE, P.; SMITH, M. Transgenic plants: methods and protocols. Berlim, Alemanha: Springer Science+Business Media, 2019. (Methods in Molecular Biology, v. 1864). p. 49-64| Biblioteca(s): Embrapa Agroenergia. |
|    |
| 5. | | OYA, T.; MARTINS, P. K.; CASAGRANDE, E. C.; LUGLE, S. M.; NEPOMUCENO, A. L.; NEUMAIER, N.; FARIAS, J. R. B. Agronomic, physiological and molecular characteristics of drought-tolerant Brazilian soybean cultivars. In: EMBRAPA SOYBEAN/JICA-CETAPAR/INTA-EEA/JIRCAS JOINT WORKSHOP ON SOYBEAN IMPROVEMENT, PRODUCTION AND UTILIZATION IN SOUTH AMERICA, 2001, Londrina. Abstracts and program. Londrina: Embrapa Soybean: JIRCAS, 2001. p. 17. (Embrapa Soja. Documentos, 170).| Biblioteca(s): Embrapa Soja. |
| |
| 6. | | RIBEIRO, A. P.; MARTINS, P. K.; DIAS, B. B. A.; KOBAYASHI, A. K.; ANDRADE, A. C.; MOLINARI, H. B. C. Overexpression of the MATE gene in Setaria viridis. In: INTERNATIONAL SERTARIA GENETICS CONFERENCE, 1., 2014, Beijing, China. [Beijing]: China Agricultural Research Systems, 2014. p. 11.| Biblioteca(s): Embrapa Agroenergia. |
| |
| 7. | | MARTINS, P. K.; DIAS, B. B. A.; RIBEIRO, A. P.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Setaria viridis: a tool for functional gene analysis in sugarcane. In: INTERNATIONAL SERTARIA GENETICS CONFERENCE, 1., 2014, Beijing, China. [Beijing]: China Agricultural Research Systems, 2014. p. 19.| Biblioteca(s): Embrapa Agroenergia. |
| |
| 10. | | MAFRA, V.; MARTINS, P. K.; FRANSCISCO, C. S.; RIBEIRO-ALVES, M.; ASTUA, J. de F.; MACHADO, M. A. Candidatus Liberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotype. In; INTERNATIONAL RESEARCH CONFERENCE ON HUANGLONGBING , 3., 2013, Orlando, Flórida, USA. IRC HLB III. Proceedings. Orlando, Flórida, USA, 2013, (online).| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 12. | | MARTINS, P. K.; DIAS, B. B. A.; RIBEIRO, A. P.; SOUTO, B. de M.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Isolation, in silico analysis and transformation of Setaria viridis with a sugarcane senescence-associated promoter. In: INTERNATIONAL SERTARIA GENETICS CONFERENCE, 1., 2014, Beijing, China. [Beijing]: China Agricultural Research Systems, 2014. p. 25.| Biblioteca(s): Embrapa Agroenergia. |
| |
| 13. | | SOUSA, C. A. F. de; DIAS, B. B. A.; MARTINS, P. K.; MOLINARI, H. B. C.; KOBAYASHI, A. K.; SOUZA JUNIOR, M. T. Nova abordagem para a fenotipagem de plantas: conceitos, ferramentas e perspectivas. Revista Brasileira de Geografia Física, v. 8, p. 660-672, 2015.| Biblioteca(s): Embrapa Agroenergia. |
| |
| 14. | | DIAS, B. B. A.; KOBAYASHI, A. K.; GOMES, A. de P. G.; RODRIGUES, D. de S.; MOLINARI, H. B. C.; FAVARO, L. C. de L.; MARTINS, P. K. Manual de Biossegurança da Embrapa Agroenergia (CQB 345/12). Brasília, DF: Embrapa Agroenergia, 2015. 35 p. ( Embrapa Agroenergia. Documentos, 19).| Biblioteca(s): Embrapa Agroenergia; Embrapa Unidades Centrais. |
|  |
| 15. | | MILORI, D. M. B. P.; BOAS, P. R. V.; VEMÂNCIO, A. L.; FERREIRA, E. J.; MARTINS, P. K.; FREITAS-ASTUA, J.; BRESOLIN, J.; CARDINALI, M. C. B. Diagnóstico de Citrus Greening (HLB) utilizando espectroscopia de fluorescência induzida por laser (LIFS). In: INAMASU, R. Y.; NAIME, J. de M.; RESENDE, A. V. de; BASSOI, L. H.; BERNARDI, A. de C. (Ed.). Agricultura de precisão: um novo olhar. São Carlos, SP: Embrapa Instrumentação, 2011. p. 78-81.| Biblioteca(s): Embrapa Instrumentação. |
|  |
| 16. | | MARTINS, P. K.; JORDÃO, B. Q.; YAMANAKA, N.; FARIAS, J. R. B.; BENEVENTI, M. A.; BINNECK, E.; FUGANTI, R.; STOLF, R.; NEPOMUCENO, A. L. Differential gene expression and mitotic cell analysis of the drought tolerant soybean (Glycine max L. Merrill Fabales, Fabaceae) cultivar MG/BR46 (Conquista) under two water deficit induction systems. Genetics and Molecular Biology, Ribeirão Preto, v. 31, n. 2, p. 512-521, 2008.| Biblioteca(s): Embrapa Soja. |
| |
| 17. | | PEDROSO, J. C.; NEPOMUCENO, A. L.; SILVA, J. F. V.; CARNEIRO, N. P.; MARTINS, P. K.; BRETON, M. C.; MARIN, S. R. R.; BINNECK, E. Differential gene expression in nematode resistant soybean genotypes. Nematology, Boston, v. 4, n. 2, p. 235, 2002. Presented in Fourth International Congress of Nematology. Programme and abstracts. 8-13 June 2002, Tenbel, La Galletas, Arona, Tenerife, Canary Islands, Spain.| Biblioteca(s): Embrapa Soja. |
| |
| 18. | | CORREA, P. H.; MARTINS, P. K.; ARENA, G. D.; MIGUEL, M. C.; MUTTI, H. S.; MACHADO, M. A.; FREITAS-ASTUA, J. Diagnóstico molecular de huanglongbing dos citros, via qPCR, em mudas de valência sobre Swingle. In:CONGRESSO BRASILEIRO DE FRUTICULTURA, 21., 2010, Natal. Frutas: saúde, inovação e responsabilidade: anais. Natal: Sociedade Brasileira de Fruticultura, 2010. pdf 1867| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 19. | | Ribeiro, A. P.; Martins, P. K.; DIAS, B. B. A.; ANDRADE, A. C.; MAGALHAES, J. V. de; KOBAYASHI, A. K.; MOLINARI, H. B. C. MATE: a promising gene for aluminum tolerance in sugarcane. In: GERMPLASM AND BREEDING, 11.; MOLECULAR BIOLOGY ISSCT WORKSHOP, 8., 2015, Saint-Gilles Réunion Island. Pushing the frontiers of sugarcane improvement: abstract. [S.l]: Ercane, 2015.| Biblioteca(s): Embrapa Agroenergia. |
| |
| 20. | | MARTINS, P. K.; NAKAYAMA, T. J.; RIBEIRO, A. P.; DIAS, B. B. A.; NEPOMUCENO, A. L.; HARMON, F. G.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Setaria viridis floral-dip: a simple and rapid Agrobacterium-mediated transformation method. Biotechnology Reports, v. 6, p. 61-63, 2015.| Biblioteca(s): Embrapa Agroenergia. |
| |
| Registros recuperados : 77 | |
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
27/04/2011 |
|
Data da última atualização: |
29/04/2011 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
MARTINS, P. K.; MAFRA, V. S.; KISHI, L. T.; FREITAS-ASTUA, J. |
|
Afiliação: |
Polyanna Kelly Martins, APTA; Valéria S. Mafra, APTA; Luciano T. Kishi, APTA; JULIANA DE FREITAS ASTUA, CNPMF. |
|
Título: |
Phylogenetic and comparative gene expression analysis of citrus WRKY transcription factor family. |
|
Ano de publicação: |
2011 |
|
Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. 1 CD-ROM. |
|
Idioma: |
Inglês |
|
Notas: |
pdf 35130 |
|
Conteúdo: |
Plants have a variety of active defense mechanisms to protect themselves from pathogen infection. Plant defense response results from the transcriptional activation of a large number of genes upon pathogen infection or treatment with pathogen elicitors. Among them, WRKY proteins are considered important transcriptional regulators in defense signaling, including the activation of SAR (systemic acquired resistance). Several genes regulating plant defense, such as NPR1 and PR (Pathogenesis-related proteins), have W-box elements in their promoters that are specifically recognized by WRKY proteins also required for induction or expression. The defining feature of WRKY transcription factors is their DNA binding domain. The WRKY domain is about 60 residues in length that contains the WRKY signature and also an atypical zinc-finger structure at the C-terminus (Cx4?5Cx22?23HxH or Cx7Cx23HxC). The aim of this work was to identify all potential WRKY transcription factors in citrus, retrieved from the CitEST (Citrus ESTs) database and to construct a phylogenetic tree with orthologs from Arabidopsis thaliana and Oryza sativa. To find ESTs coding for WRKY proteins in citrus we performed a tBLASTn search on the CitEST database. The WRKY domain sequences of seven Arabidopsis WRKY family members each representing one of the WRKY subgroups were used as query sequences. Phylogenetic tree were constructed by the neighbor-joining (NJ) method in MEGA 4. NJ analysis was performed with the Pairwise Deletion option and the Poisson correction. For statistical reliability, bootstrap analysis was conducted with 1,000 replicates to assess statistical support for each node. The primary search resulted in 71 non-redundant hits, of which 9 were removed as they did not contain the conserved WRKY domain signature. The remaining 62 sequences were used to construct the phylogenetic tree; this analysis identified 19 unigenes for the group 1; 6 unigenes for the subgroup 2a, 3 unigenes for the subgroup 2b, 12 unigenes for the subgroup 2c, 13 unigenes for the group 2d, 9 unigenes for subgroup 2e and 7 unigenes for the group 3. To complete the phylogenetic analysis, WRKY sequences from the draft sequencing of the genomes of Citrus clementina and C. sinensis will be included. We are particularly interested in WRKY proteins involved in biotic stress, so the next step will be to evaluate the transcriptional profile of some WRKY genes under different biotic stress conditions. This study will be crucial to select candidates for citrus genetic transformation. MenosPlants have a variety of active defense mechanisms to protect themselves from pathogen infection. Plant defense response results from the transcriptional activation of a large number of genes upon pathogen infection or treatment with pathogen elicitors. Among them, WRKY proteins are considered important transcriptional regulators in defense signaling, including the activation of SAR (systemic acquired resistance). Several genes regulating plant defense, such as NPR1 and PR (Pathogenesis-related proteins), have W-box elements in their promoters that are specifically recognized by WRKY proteins also required for induction or expression. The defining feature of WRKY transcription factors is their DNA binding domain. The WRKY domain is about 60 residues in length that contains the WRKY signature and also an atypical zinc-finger structure at the C-terminus (Cx4?5Cx22?23HxH or Cx7Cx23HxC). The aim of this work was to identify all potential WRKY transcription factors in citrus, retrieved from the CitEST (Citrus ESTs) database and to construct a phylogenetic tree with orthologs from Arabidopsis thaliana and Oryza sativa. To find ESTs coding for WRKY proteins in citrus we performed a tBLASTn search on the CitEST database. The WRKY domain sequences of seven Arabidopsis WRKY family members each representing one of the WRKY subgroups were used as query sequences. Phylogenetic tree were constructed by the neighbor-joining (NJ) method in MEGA 4. NJ analysis was performed with the Pairwise... Mostrar Tudo |
|
Palavras-Chave: |
CitEST; SAR; W-box. |
|
Thesaurus NAL: |
Citrus; transcription factors. |
|
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
Marc: |
LEADER 03255nam a2200217 a 4500
001 1886952
005 2011-04-29
008 2011 bl uuuu u00u1 u #d
100 1 $aMARTINS, P. K.
245 $aPhylogenetic and comparative gene expression analysis of citrus WRKY transcription factor family.
260 $aIn: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. 1 CD-ROM.$c2011
500 $apdf 35130
520 $aPlants have a variety of active defense mechanisms to protect themselves from pathogen infection. Plant defense response results from the transcriptional activation of a large number of genes upon pathogen infection or treatment with pathogen elicitors. Among them, WRKY proteins are considered important transcriptional regulators in defense signaling, including the activation of SAR (systemic acquired resistance). Several genes regulating plant defense, such as NPR1 and PR (Pathogenesis-related proteins), have W-box elements in their promoters that are specifically recognized by WRKY proteins also required for induction or expression. The defining feature of WRKY transcription factors is their DNA binding domain. The WRKY domain is about 60 residues in length that contains the WRKY signature and also an atypical zinc-finger structure at the C-terminus (Cx4?5Cx22?23HxH or Cx7Cx23HxC). The aim of this work was to identify all potential WRKY transcription factors in citrus, retrieved from the CitEST (Citrus ESTs) database and to construct a phylogenetic tree with orthologs from Arabidopsis thaliana and Oryza sativa. To find ESTs coding for WRKY proteins in citrus we performed a tBLASTn search on the CitEST database. The WRKY domain sequences of seven Arabidopsis WRKY family members each representing one of the WRKY subgroups were used as query sequences. Phylogenetic tree were constructed by the neighbor-joining (NJ) method in MEGA 4. NJ analysis was performed with the Pairwise Deletion option and the Poisson correction. For statistical reliability, bootstrap analysis was conducted with 1,000 replicates to assess statistical support for each node. The primary search resulted in 71 non-redundant hits, of which 9 were removed as they did not contain the conserved WRKY domain signature. The remaining 62 sequences were used to construct the phylogenetic tree; this analysis identified 19 unigenes for the group 1; 6 unigenes for the subgroup 2a, 3 unigenes for the subgroup 2b, 12 unigenes for the subgroup 2c, 13 unigenes for the group 2d, 9 unigenes for subgroup 2e and 7 unigenes for the group 3. To complete the phylogenetic analysis, WRKY sequences from the draft sequencing of the genomes of Citrus clementina and C. sinensis will be included. We are particularly interested in WRKY proteins involved in biotic stress, so the next step will be to evaluate the transcriptional profile of some WRKY genes under different biotic stress conditions. This study will be crucial to select candidates for citrus genetic transformation.
650 $aCitrus
650 $atranscription factors
653 $aCitEST
653 $aSAR
653 $aW-box
700 1 $aMAFRA, V. S.
700 1 $aKISHI, L. T.
700 1 $aFREITAS-ASTUA, J.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
