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| Registros recuperados : 195 | |
| 27. |  | MARTINS, C. F.; DODE, M.; PEREIRA, D. C.; RUMPF, R. Criopreservação de espermatozóide do epidídimo de animais mortos e sua utilização na produção de embriões in vitro: uma importante ferramenta na preservação de genes valiosos. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 8., 2003, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2003. p. 151.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 30. | | MARTINS, C. F.; SERENO, J. R. B.; PIRES, N. L. Calf production by artificial insemination with spermatozoa obtained from epididymides refrigerated at 5°C for long periods after death. Acta Scientiae Veterinariae, Porto Alegre, v. 38, supl. 2, p. s815, 2010. Abstract 223. Edição dos resumos da 25. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Porto de Galinhas, PE, 2010. p. S815| Biblioteca(s): Embrapa Cerrados. |
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| 32. | | CUNHA, E. R.; SILVA, C. G.; GUIMARÃES, C. O.; MARTINS, C. F. Comparative study of different evaluations of cryopreserved bovine semen imported by Brazil. Animal Reproduction, v. 7, n. 3, p. 284, Jul./Sept. 2010. Edição dos resumos do International Symposium Animal Biology of Reproductive, 3., 2010, Águas de São Pedro.| Biblioteca(s): Embrapa Cerrados. |
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| 36. | | MOURA, M. T.; MARTINS, C. F.; BADARACO, J.; SOUSA, R. V. de; RUMPF, R. Isolamento de linhagens de fibroblastos adultos bovinos a partir de biópsia resfriada a 4º C. In: SIMPÓSIO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2008, Brasília, DF. Anais... Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. p. 527.| Biblioteca(s): Embrapa Cerrados. |
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| 37. | | MOURA, M. T.; MARTINS, C. F.; BADARACO, J.; SOUSA, R. V. de; RUMPF, R. Isolamento de linhagens de fibroblastos adultos bovinos a partir de bióspia resfriada a 4º C. In: SIMPÓSIO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2008, Brasília, DF. Anais... Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia: Fundação de Apoio à Pesquisa Científica e Tecnológica - FUNCREDI, 2008. p.527.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 38. | | MOURA, M. T.; MARTINS, C. F.; BADARACO, J.; SOUSA, R. V.; RUMPF, R. Isolamento de linhagens de fibroblastos adultos a partir de biópsia resfriada a 4ºC. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 13., 2008, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. Resumo 060. p. 102.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 39. | | CUNHA, E. R.; SILVA, C. G.; SIQUEIRA, L. G. B.; MARTINS, C. F. Isolation, culture and cryopreservation of bovine amniotic cells with Dimethyl sulfoxide, Dimethylformamide or Glycerol. Animal Reproduction, v. 7, n. 3, p. 317, Jul./Sept. 2010. Edição dos resumos do III International Symposium Animal Biology of Reproductive, 3., 2010, Águas de São Pedro.| Biblioteca(s): Embrapa Cerrados. |
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| Registros recuperados : 195 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
30/12/2020 |
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Data da última atualização: |
30/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
ARANTES, L. G.; TONELLI, G. S. S. S.; MARTINS, C. F. |
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Afiliação: |
CARLOS FREDERICO MARTINS, CPAC. |
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Título: |
Cellular Characterization and Effects of Cryoprotectant Solutions on the Viability of Fibroblasts from Three Brazilian Wild Cats. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
BIOPRESERVATION AND BIOBANKING, v.0, n. 0, 2020. |
|
Páginas: |
8 p. |
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Idioma: |
Português |
|
Conteúdo: |
Preserving genetic material in cryogenic conditions presents a viable alternative for the protection of species? gene variability. However, there is an enormous need to establish and test cryopreservation protocols that are suitable for each diverse cell type to guarantee technical success in the long run. Considering this, fibroblasts from jaguar (Panthera onca), oncilla (Leopardus tigrinus), and pampas cat (Leopardus colocolo) were subjected to cell characterization and then cryopreservation in different cryoprotectant solutions (2.5%, 10% dimethyl sulfoxide [DMSO] or CryoSOfree). Further testing was conducted to determine each solution?s performance in preserving cell viability. In culture, a growth curve to assess cellular growth potential showed that exponential proliferation lasts for about the first 50 hours of in vitro culturing, declining in pace afterward. L. colocolo and L. tigrinus presented no difference in cell viability while using 2.5% DMSO protocols. P. onca cells did not present difference on viability for both concentrations of DMSO. Protocols using CryoSOfree resulted in a decreased viability of P. onca fibroblasts. Morphological differences between fibroblasts among the species were noted under bright field microscopy and scanning electron microscopy. L. colocolo and P. onca cells are fusiform, and L. tigrinus are spherical. All cells presented cytoplasmic projections. Transmission electron microscopy revealed vacuoles and secretion granules, indicating intense cell activity after thawing. Differences found in the efficiency of cryopreservation protocols according to the type of cryoprotectant indicate that species react differently to freezing and thawing processes. This research evaluates key aspects of in vitro protocols for cryopreservation of wild animals, which need to be optimized to guarantee successful cell culturing. More suitable protocols lead to increased efficiency in establishing fibroblast cryobanks and also facilitating the use of wild cats? cells in cloning techniques, contributing directly to preserving wild fauna. MenosPreserving genetic material in cryogenic conditions presents a viable alternative for the protection of species? gene variability. However, there is an enormous need to establish and test cryopreservation protocols that are suitable for each diverse cell type to guarantee technical success in the long run. Considering this, fibroblasts from jaguar (Panthera onca), oncilla (Leopardus tigrinus), and pampas cat (Leopardus colocolo) were subjected to cell characterization and then cryopreservation in different cryoprotectant solutions (2.5%, 10% dimethyl sulfoxide [DMSO] or CryoSOfree). Further testing was conducted to determine each solution?s performance in preserving cell viability. In culture, a growth curve to assess cellular growth potential showed that exponential proliferation lasts for about the first 50 hours of in vitro culturing, declining in pace afterward. L. colocolo and L. tigrinus presented no difference in cell viability while using 2.5% DMSO protocols. P. onca cells did not present difference on viability for both concentrations of DMSO. Protocols using CryoSOfree resulted in a decreased viability of P. onca fibroblasts. Morphological differences between fibroblasts among the species were noted under bright field microscopy and scanning electron microscopy. L. colocolo and P. onca cells are fusiform, and L. tigrinus are spherical. All cells presented cytoplasmic projections. Transmission electron microscopy revealed vacuoles and secretion granules, indicating ... Mostrar Tudo |
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Thesagro: |
Conservação; Criopreservação; Germoplasma. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02696naa a2200193 a 4500
001 2128861
005 2020-12-30
008 2020 bl uuuu u00u1 u #d
100 1 $aARANTES, L. G.
245 $aCellular Characterization and Effects of Cryoprotectant Solutions on the Viability of Fibroblasts from Three Brazilian Wild Cats.$h[electronic resource]
260 $c2020
300 $a8 p.
520 $aPreserving genetic material in cryogenic conditions presents a viable alternative for the protection of species? gene variability. However, there is an enormous need to establish and test cryopreservation protocols that are suitable for each diverse cell type to guarantee technical success in the long run. Considering this, fibroblasts from jaguar (Panthera onca), oncilla (Leopardus tigrinus), and pampas cat (Leopardus colocolo) were subjected to cell characterization and then cryopreservation in different cryoprotectant solutions (2.5%, 10% dimethyl sulfoxide [DMSO] or CryoSOfree). Further testing was conducted to determine each solution?s performance in preserving cell viability. In culture, a growth curve to assess cellular growth potential showed that exponential proliferation lasts for about the first 50 hours of in vitro culturing, declining in pace afterward. L. colocolo and L. tigrinus presented no difference in cell viability while using 2.5% DMSO protocols. P. onca cells did not present difference on viability for both concentrations of DMSO. Protocols using CryoSOfree resulted in a decreased viability of P. onca fibroblasts. Morphological differences between fibroblasts among the species were noted under bright field microscopy and scanning electron microscopy. L. colocolo and P. onca cells are fusiform, and L. tigrinus are spherical. All cells presented cytoplasmic projections. Transmission electron microscopy revealed vacuoles and secretion granules, indicating intense cell activity after thawing. Differences found in the efficiency of cryopreservation protocols according to the type of cryoprotectant indicate that species react differently to freezing and thawing processes. This research evaluates key aspects of in vitro protocols for cryopreservation of wild animals, which need to be optimized to guarantee successful cell culturing. More suitable protocols lead to increased efficiency in establishing fibroblast cryobanks and also facilitating the use of wild cats? cells in cloning techniques, contributing directly to preserving wild fauna.
650 $aConservação
650 $aCriopreservação
650 $aGermoplasma
700 1 $aTONELLI, G. S. S. S.
700 1 $aMARTINS, C. F.
773 $tBIOPRESERVATION AND BIOBANKING$gv.0, n. 0, 2020.
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