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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
23/10/2017 |
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Data da última atualização: |
23/10/2017 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
CERDEIRA, A. L.; SANTOS, N. A. G.; PESSOA, M. C. P. Y.; SMITH JR, S.; LANCHOTE, V. L. |
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Afiliação: |
ANTONIO LUIZ CERDEIRA, CNPMA; USP; MARIA CONCEICAO PERES YOUNG PESSOA, CNPMA; USDA-ARS/USA; USP. |
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Título: |
Presence of Atrazine in Water in a Recharge Area of Guarany Aquifer in Brazil |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
In: ANNUAL MISSISSIPPI WATER RESOURCES CONFERENCE, 34., 2004, Jackson. Proceedings... Raymond: Mississippi Sate University: Mississippi Water Resources Research Institute, 2004. p. 43-46. |
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Idioma: |
Inglês |
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Conteúdo: |
The region of Ribeirao Preto City located in Sao Paulo State, southeastern Brazil, is an important sugarcane, soybean and corn producing area. This region is also an important recharge area for groundwater of the Guarany aquifer, a water supply source of the city and region. The cultivation of grain and sugar cane in thes area demands the frequent use of the herbicide atrazine (2-chloro-4- (ethylamino)-6-(isopropylamino)-S-triazine). This research was conducted to characterize the potential contamination of groundwater with atrazine. Surface water samples were collected in the Espraiado stream in a selected watershed on the area, during the years of 1995-1998. Groundwater was also collected in wells located at the edge of the watershed during the years of 1999 to 2002. The water samples were analyzed by HPLC (High Performance Liquid Chromatography) procedure followed by GC-MS form confirmation. To predict the atrazine leaching in the area, the CMLS-94 (Chemical Movement Layered Soil) simulation model was also used. Only four atrazine detections in surface water were found, however, none of them were confirmed with GC-MS. No atrazine was detected in groundwater samples. The results obtained by the CMLS-94 simulations predicted that atrazine, after four years from the application date, would not have reached the depth of the confined aquifer (40m). |
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Palavras-Chave: |
Guarany Aquifer. |
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Thesaurus Nal: |
atrazine; Brazil; water. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/165398/1/2004SP-87-Cerdeira-Presence-15768.pdf
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Marc: |
LEADER 02098nam a2200205 a 4500
001 2077901
005 2017-10-23
008 2004 bl uuuu u00u1 u #d
100 1 $aCERDEIRA, A. L.
245 $aPresence of Atrazine in Water in a Recharge Area of Guarany Aquifer in Brazil$h[electronic resource]
260 $aIn: ANNUAL MISSISSIPPI WATER RESOURCES CONFERENCE, 34., 2004, Jackson. Proceedings... Raymond: Mississippi Sate University: Mississippi Water Resources Research Institute, 2004. p. 43-46.$c2004
520 $aThe region of Ribeirao Preto City located in Sao Paulo State, southeastern Brazil, is an important sugarcane, soybean and corn producing area. This region is also an important recharge area for groundwater of the Guarany aquifer, a water supply source of the city and region. The cultivation of grain and sugar cane in thes area demands the frequent use of the herbicide atrazine (2-chloro-4- (ethylamino)-6-(isopropylamino)-S-triazine). This research was conducted to characterize the potential contamination of groundwater with atrazine. Surface water samples were collected in the Espraiado stream in a selected watershed on the area, during the years of 1995-1998. Groundwater was also collected in wells located at the edge of the watershed during the years of 1999 to 2002. The water samples were analyzed by HPLC (High Performance Liquid Chromatography) procedure followed by GC-MS form confirmation. To predict the atrazine leaching in the area, the CMLS-94 (Chemical Movement Layered Soil) simulation model was also used. Only four atrazine detections in surface water were found, however, none of them were confirmed with GC-MS. No atrazine was detected in groundwater samples. The results obtained by the CMLS-94 simulations predicted that atrazine, after four years from the application date, would not have reached the depth of the confined aquifer (40m).
650 $aatrazine
650 $aBrazil
650 $awater
653 $aGuarany Aquifer
700 1 $aSANTOS, N. A. G.
700 1 $aPESSOA, M. C. P. Y.
700 1 $aSMITH JR, S.
700 1 $aLANCHOTE, V. L.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
11/12/2020 |
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Data da última atualização: |
11/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
MARQUES, T. C.; SANTOS, E. C. da S.; DIESEL, T. O.; MARTINS, C. F.; CUMPA, H. C. B.; LEME, L. de O.; DODE, M. A. N.; ALVES, B. G.; COSTA, F. P. H.; OLIVEIRA, E. B.; GAMBARINI, M. L. |
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Afiliação: |
CARLOS FREDERICO MARTINS, CPAC; MARGOT ALVES NUNES DODE, Cenargen. |
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Título: |
Blastocoel fluid removal and melatonin supplementation in the culture medium improve the viability of vitrified bovine embryos. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Theriogenology, Vv. 160, p. 134-141, 2021. |
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Páginas: |
p. 134-141 |
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ISSN: |
0093-691X |
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Idioma: |
Português |
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Conteúdo: |
In this study, we investigated the effects of melatonin supplementation in the culture medium and blastocoel fluid removal (BFR) before vitrification on the quality and viability of in vitro-derived bovine embryos. After fertilization, presumptive zygotes were assigned to one of the following treatments: control, in vitro standard culture (IVC) medium; IVC þ M109 , IVC medium supplemented 109 M melatonin; or IVC þ M109 BFR, IVC medium supplemented with 109 M melatonin plus BFR on day 7 (D7) of culture. D7 blastocysts were vitrified by the Cryotop method and, after 5 mo of storage, were warmed and incubated for an additional 72 h. The re-expansion rate was evaluated after 2 and 24 h, and the hatching rate was evaluated after 24, 48, and 72 h. At 72 h, the total number of cells (TNC); number of apoptotic cells (NAC); and expression of genes related to oxidative stress (HSPA5), cell metabolism (SLC2A3), cell repair (MSH6), placentation (KRT8 and PLAC8), and implantation (FOSL1) were assessed in the blastocysts. Less than 30% of the control blastocysts re-expanded until 2 h, whereas more than 85% of the IVC þ M109 and IVC þ M109 BFR blastocysts re-expanded (P < 0.05). The hatching rate of IVC þ M109 BFR blastocysts increased at all time points (P < 0.05), reaching 66.8% at 72 h of incubation. The TNC was similar among treatments (P > 0.05), regardless of vitrification/warming and re-cultivation. The NAC:TNC was smaller for melatonin-treated blastocysts (P < 0.05). BFR increased HSPA5 (P ¼ 0.0118) expression and did not affect SLC2A3, MSH6, KRT8, and FOSL1 expression (P > 0.05). In conclusion, melatonin (109 M) supplementation in the culture medium and BFR on D7 of culture increased the hatching rate 24, 48, and 72 h after warming of the vitrified embryos, indicating an improvement in cryotolerance. MenosIn this study, we investigated the effects of melatonin supplementation in the culture medium and blastocoel fluid removal (BFR) before vitrification on the quality and viability of in vitro-derived bovine embryos. After fertilization, presumptive zygotes were assigned to one of the following treatments: control, in vitro standard culture (IVC) medium; IVC þ M109 , IVC medium supplemented 109 M melatonin; or IVC þ M109 BFR, IVC medium supplemented with 109 M melatonin plus BFR on day 7 (D7) of culture. D7 blastocysts were vitrified by the Cryotop method and, after 5 mo of storage, were warmed and incubated for an additional 72 h. The re-expansion rate was evaluated after 2 and 24 h, and the hatching rate was evaluated after 24, 48, and 72 h. At 72 h, the total number of cells (TNC); number of apoptotic cells (NAC); and expression of genes related to oxidative stress (HSPA5), cell metabolism (SLC2A3), cell repair (MSH6), placentation (KRT8 and PLAC8), and implantation (FOSL1) were assessed in the blastocysts. Less than 30% of the control blastocysts re-expanded until 2 h, whereas more than 85% of the IVC þ M109 and IVC þ M109 BFR blastocysts re-expanded (P < 0.05). The hatching rate of IVC þ M109 BFR blastocysts increased at all time points (P < 0.05), reaching 66.8% at 72 h of incubation. The TNC was similar among treatments (P > 0.05), regardless of vitrification/warming and re-cultivation. The NAC:TNC was smaller for melatonin-treated blastocysts (P < 0.05). BFR increased ... Mostrar Tudo |
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Palavras-Chave: |
Apoptose celular; Criotolerância; Fertilização; Melatonina; Vitrificação. |
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Thesagro: |
Cultura de Embrião; Embrião. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02843naa a2200349 a 4500
001 2127986
005 2020-12-11
008 2021 bl uuuu u00u1 u #d
022 $a0093-691X
100 1 $aMARQUES, T. C.
245 $aBlastocoel fluid removal and melatonin supplementation in the culture medium improve the viability of vitrified bovine embryos.$h[electronic resource]
260 $c2021
300 $ap. 134-141
520 $aIn this study, we investigated the effects of melatonin supplementation in the culture medium and blastocoel fluid removal (BFR) before vitrification on the quality and viability of in vitro-derived bovine embryos. After fertilization, presumptive zygotes were assigned to one of the following treatments: control, in vitro standard culture (IVC) medium; IVC þ M109 , IVC medium supplemented 109 M melatonin; or IVC þ M109 BFR, IVC medium supplemented with 109 M melatonin plus BFR on day 7 (D7) of culture. D7 blastocysts were vitrified by the Cryotop method and, after 5 mo of storage, were warmed and incubated for an additional 72 h. The re-expansion rate was evaluated after 2 and 24 h, and the hatching rate was evaluated after 24, 48, and 72 h. At 72 h, the total number of cells (TNC); number of apoptotic cells (NAC); and expression of genes related to oxidative stress (HSPA5), cell metabolism (SLC2A3), cell repair (MSH6), placentation (KRT8 and PLAC8), and implantation (FOSL1) were assessed in the blastocysts. Less than 30% of the control blastocysts re-expanded until 2 h, whereas more than 85% of the IVC þ M109 and IVC þ M109 BFR blastocysts re-expanded (P < 0.05). The hatching rate of IVC þ M109 BFR blastocysts increased at all time points (P < 0.05), reaching 66.8% at 72 h of incubation. The TNC was similar among treatments (P > 0.05), regardless of vitrification/warming and re-cultivation. The NAC:TNC was smaller for melatonin-treated blastocysts (P < 0.05). BFR increased HSPA5 (P ¼ 0.0118) expression and did not affect SLC2A3, MSH6, KRT8, and FOSL1 expression (P > 0.05). In conclusion, melatonin (109 M) supplementation in the culture medium and BFR on D7 of culture increased the hatching rate 24, 48, and 72 h after warming of the vitrified embryos, indicating an improvement in cryotolerance.
650 $aCultura de Embrião
650 $aEmbrião
653 $aApoptose celular
653 $aCriotolerância
653 $aFertilização
653 $aMelatonina
653 $aVitrificação
700 1 $aSANTOS, E. C. da S.
700 1 $aDIESEL, T. O.
700 1 $aMARTINS, C. F.
700 1 $aCUMPA, H. C. B.
700 1 $aLEME, L. de O.
700 1 $aDODE, M. A. N.
700 1 $aALVES, B. G.
700 1 $aCOSTA, F. P. H.
700 1 $aOLIVEIRA, E. B.
700 1 $aGAMBARINI, M. L.
773 $tTheriogenology, Vv. 160, p. 134-141, 2021.
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