Data/hora: 03/06/2024 06:35:14

Biblioteca(s): | Embrapa Cerrados.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | B - 1
Data corrente: | 19/09/2022
Data da última atualização: | 19/09/2022
Autoria: | MATTOS, L. M.; REIS, F. C.; RACANICCI, A. M. C.; PIVATO, I.; TONELLI, G. S. S. S.; BÁO, S. N.; MARTINS, C. F.
Afiliação: | LUCIANA MIRANDA MATTOS; FILIPE CARNEIRO REIS; ALINE M.C. RACANICCI; IVO PIVATO; GUILHERME S.S.S. TONELLI; SÔNIA NAIR BÁO; CARLOS FREDERICO MARTINS, CPAC.
Título: | Freezing for the Future: obtaining fibroblast samples from deceased wild mammals for the Brazilian Cerrado Germplasm Bank.
Ano de publicação: | 2022
Fonte/Imprenta: | Biopreservation and Biobanking, ahead of print, 2022.
DOI: | DOI: 10.1089/bio.2022.0039
Idioma: | Inglês
Conteúdo: | We isolated and further characterized fibroblasts obtained from postmortem skin biopsies of three different Brazilian wild species (Chrysocyon brachyurus?maned wolf, Cerdocyon thous?crab-eating fox, Mazama gouazoubira?brown brocket deer). The effects of two cryoprotectants, 10% dimethyl sulfoxide (DMSO) and 5% dimethylformamide (DMF), were assessed to determine the most efficient cryopreservation protocol. Such an investigation promotes the creation of germplasm banks, using samples that would otherwise be rejected and permanently lost following the death of the animals. We utilized animal corpses that were involved in highway accidents, found dead in the natural environment, or referred to us from the veterinary hospital at the Bras&#305; &#769;lia Zoo. Fibroblasts from C. brachyurus specimens presented a delay in cell growth in Dulbecco?s modified Eagle?s medium in relation to other species. This observation is a limiting factor for the future storage of cells from this species. Differences in cellular morphology were observed between C. brachyurus, C. thous, and M. goua- zoubira, presenting branched, fusiform, and spherical forms, respectively. The cryoprotective solution con- taining 10% DMSO was more efficient than 5% DMF medium in preserving the viability of fibroblasts of the three species ( p < 0.05). After defining the best cryopreservation solution, a germplasm bank was successfully formed. This biological reservoir is configured as the first germplasm bank containing somatic cells and gametes of wild mammals of the Cerrado biome of Brazil. This material will be used for future characterization of the species and multiplication by means of nuclear transfer cloning.
Thesagro: | Animal Selvagem; Banco de Germoplasma; Biotecnologia; Criopreservação.
Thesaurus Nal: | Biotechnology; Cryopreservation; Germplasm; Wild animals.
Categoria do assunto: | --
Registro original: | Embrapa Cerrados (CPAC)
Exemplares: | CPAC - (37360-1) - UPC - AP - DD - DIGITAL -  - DIGITAL - DIS