Data/hora: 14/06/2024 08:01:53

Biblioteca(s): | Embrapa Cerrados.
Data corrente: | 06/06/2023
Data da última atualização: | 06/06/2023
Autoria: | SILVA, C. G.; MARTINS, C. F.
Afiliação: | CAROLINA GONZALES DA SILVA; CARLOS FREDERICO MARTINS, CPAC.
Título: | Stem cells as nuclear donors for mammalian cloning.
Ano de publicação: | 2023
Fonte/Imprenta: | In: MOURA, M. T. (ed.). Somatic cell nuclear transfer technology. São Paulo: Humana Press, 2023.
Páginas: | p. 115-129
Série: | (Springer. Methods in Molecular Biology, 2647).
Idioma: | Inglês
Conteúdo: | Mammals are routinely cloned by introducing somatic nuclei into enucleated oocytes. Cloning contributes to propagating desired animals, to germplasm conservation efforts, among other applications. A challenge to more broader use of this technology is the relatively low cloning efficiency, which inversely correlates with donor cell differentiation status. Emerging evidence suggests that adult multipotent stem cells improve cloning efficiency, while the greater potential of embryonic stem cells for cloning remains restricted to the mouse. The derivation of pluripotent or totipotent stem cells from livestock and wild species and their association with modulators of epigenetic marks in donor cells should increase cloning efficiency.
Palavras-Chave: | Células tronco; Conservação de germoplasma.
Thesagro: | Clone; Clone Nucelar; Mamífero.
Categoria do assunto: | --
Registro original: | Embrapa Cerrados (CPAC)
Exemplares: | CPAC - (37594-1) - UPC - PL - DD - DIGITAL -  - DIGITAL - DIS