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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Soja. |
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Data corrente: |
02/10/2015 |
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Data da última atualização: |
13/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARCOLINO-GOMES, J.; RODRIGUES, F. A.; FUGANTI-PAGLIARINI, R.; NAKAYAMA, T. J.; REIS, R. R.; FARIAS, J. R. B.; HARMON, F. G.; MOLINARI, H. B. C.; MOLINARI, M. D. C.; NEPOMUCENO, A. L. |
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Afiliação: |
UEL; AUTOR; AUTOR; UFV; UEL; JOSE RENATO BOUCAS FARIAS, CNPSO; U C BERKELEY - BERKELEY; HUGO BRUNO CORREA MOLINARI, CNPAE; UEL; ALEXANDRE LIMA NEPOMUCENO, CNPSO. |
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Título: |
Transcriptome-wide identification of reference genes for expression analysis of soybean responses to drought stress along the day. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plos One, v. 10, n. 9, e0139051, 2015. |
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Páginas: |
16 p. |
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DOI: |
10.1371/journal.pone.0139051 |
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Idioma: |
Inglês |
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Conteúdo: |
The soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptomewide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean gene (DREB5) evaluated in different periods of the day. MenosThe soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptomewide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean ge... Mostrar Tudo |
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Palavras-Chave: |
Drought stress; Soybean. |
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Thesagro: |
Estiagem; Resistência à seca; Seca; Soja. |
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Thesaurus Nal: |
Drought tolerance; Soybeans; Transcriptome. |
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Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/166647/1/nepomuceno-plos-one.pdf
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Marc: |
LEADER 02592naa a2200361 a 4500
001 2079599
005 2017-11-13
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pone.0139051$2DOI
100 1 $aMARCOLINO-GOMES, J.
245 $aTranscriptome-wide identification of reference genes for expression analysis of soybean responses to drought stress along the day.$h[electronic resource]
260 $c2015
300 $a16 p.
520 $aThe soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptomewide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean gene (DREB5) evaluated in different periods of the day.
650 $aDrought tolerance
650 $aSoybeans
650 $aTranscriptome
650 $aEstiagem
650 $aResistência à seca
650 $aSeca
650 $aSoja
653 $aDrought stress
653 $aSoybean
700 1 $aRODRIGUES, F. A.
700 1 $aFUGANTI-PAGLIARINI, R.
700 1 $aNAKAYAMA, T. J.
700 1 $aREIS, R. R.
700 1 $aFARIAS, J. R. B.
700 1 $aHARMON, F. G.
700 1 $aMOLINARI, H. B. C.
700 1 $aMOLINARI, M. D. C.
700 1 $aNEPOMUCENO, A. L.
773 $tPlos One$gv. 10, n. 9, e0139051, 2015.
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Registro original: |
Embrapa Soja (CNPSO) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
07/11/2006 |
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Data da última atualização: |
27/01/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - A |
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Autoria: |
ANDRIOLI, A.; GOUVEIA, A. M. G.; MARTINS, A. de S.; PINHEIRO, R. R.; SANTOS, D. O. |
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Afiliação: |
ALICE ANDRIOLI, CNPC; Aurora Maria Guimarães Gouveia, UFMG; Almir de Sousa Martins; RAYMUNDO RIZALDO PINHEIRO, CNPC; DIONES OLIVEIRA SANTOS, CNPC. |
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Título: |
Fatores de risco na transmissão do lentivírus caprino pelo sêmen. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 41, n. 8, p. 1313-1319, ago. 2006. |
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DOI: |
10.1590/S0100-204X2006000800015 |
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Idioma: |
Português |
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Conteúdo: |
Resumo: 0 objetivo deste trabalho foi avaliar a presença do DNA pro-viral do lentivirus caprino (LVC) em ejaculados de machos infectados naturalmente, e verificar a influencia da lavagem do semen e da presença de inflamaçao testicular sobre a carga viral. Foram realizadas oito coletas de semen de sete reprodutores soropositivos para o LVC: quatro antes dos animais sofrerem dano testicular e quatro depois. Entre as coletas realizadas na mesma semana, em uma, 0 ejaculado era lavado, para retirada do plasma seminal, e na outra, nao. 0 DNA pro-viral do LVC foi identificado pela reaçao ern cadeia da polimerase Nested (PCR Nested), e pelo isolarnento viral. 0 virus foi isolado em 7,1 % das amostras. A PCR identificou 0 DNA pro-viral ern 35,7% do total das amostras: 17,9% nas amostras lavadas e 53,6% das amostras de semen integrais. 0 dano ao testiculo permite maior fluxo do virus para o semen, pois antes do dano, 21,4% das amostras foram positivas e pos-dano, 50%. A transmissao do LVC pelo semen de reprodutores caprinos e potencializada pela presença de inflamaçoes testiculares e pelo fato de o semen criopreservado conter o LVC na forma infectante.
[Risk factors in caprine lentivirus transmission through semen].
Abstract: The objective of this work was to evaluate the presence of the DNA provirus of the caprine lentivirus (LVC) in ejaculates of naturally infected males, and to verify the influence of the wash of the semen as well as the presence of testicle inflammation on the viral load. Eight semen collections of seven soropositive reproducers were accomplished, four before testicle injury and four after injury. Amongst the collections carried out at the same week, in one the ejaculate was washed, to withdraw the plasma seminal, and in the other it was not. The provirus DNA was identified both by Nested polymerase chain reaction technique (Nested PCR) and by the viral isolation. The virus was isolated in 7.1% of the samples. The PCR identified the provirus DNA in 35.7% of all samples, 17.9% in the washed samples and 53.6% of the integral semen samples. The injury of the testicle tends to greater flow of virus for the semen, therefore, before injury, 21.4% of the samples were positive and after-injury, 50%. Risk of transmission of the LVC by semen of goat reproducers is strengthened by the presence of testicle inflammations and the fact that the criopreserved semen contains the LVC in infecting form. MenosResumo: 0 objetivo deste trabalho foi avaliar a presença do DNA pro-viral do lentivirus caprino (LVC) em ejaculados de machos infectados naturalmente, e verificar a influencia da lavagem do semen e da presença de inflamaçao testicular sobre a carga viral. Foram realizadas oito coletas de semen de sete reprodutores soropositivos para o LVC: quatro antes dos animais sofrerem dano testicular e quatro depois. Entre as coletas realizadas na mesma semana, em uma, 0 ejaculado era lavado, para retirada do plasma seminal, e na outra, nao. 0 DNA pro-viral do LVC foi identificado pela reaçao ern cadeia da polimerase Nested (PCR Nested), e pelo isolarnento viral. 0 virus foi isolado em 7,1 % das amostras. A PCR identificou 0 DNA pro-viral ern 35,7% do total das amostras: 17,9% nas amostras lavadas e 53,6% das amostras de semen integrais. 0 dano ao testiculo permite maior fluxo do virus para o semen, pois antes do dano, 21,4% das amostras foram positivas e pos-dano, 50%. A transmissao do LVC pelo semen de reprodutores caprinos e potencializada pela presença de inflamaçoes testiculares e pelo fato de o semen criopreservado conter o LVC na forma infectante.
[Risk factors in caprine lentivirus transmission through semen].
Abstract: The objective of this work was to evaluate the presence of the DNA provirus of the caprine lentivirus (LVC) in ejaculates of naturally infected males, and to verify the influence of the wash of the semen as well as the presence of testicle inflammation on the v... Mostrar Tudo |
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Palavras-Chave: |
Artrite Encefalite; CAEV; Infected bucks. |
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Thesagro: |
Caprino; Reprodução animal; Sêmen; Transmissão de doença. |
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Thesaurus NAL: |
Animal reproduction; Artificial insemination; Caprine arthritis encephalitis virus; Goats; Infectious diseases; Lentivirus; Reproductive disorders; Virus transmission. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 03505naa a2200361 a 4500
001 1530230
005 2021-01-27
008 2006 bl uuuu u00u1 u #d
024 7 $a10.1590/S0100-204X2006000800015$2DOI
100 1 $aANDRIOLI, A.
245 $aFatores de risco na transmissão do lentivírus caprino pelo sêmen.
260 $c2006
520 $aResumo: 0 objetivo deste trabalho foi avaliar a presença do DNA pro-viral do lentivirus caprino (LVC) em ejaculados de machos infectados naturalmente, e verificar a influencia da lavagem do semen e da presença de inflamaçao testicular sobre a carga viral. Foram realizadas oito coletas de semen de sete reprodutores soropositivos para o LVC: quatro antes dos animais sofrerem dano testicular e quatro depois. Entre as coletas realizadas na mesma semana, em uma, 0 ejaculado era lavado, para retirada do plasma seminal, e na outra, nao. 0 DNA pro-viral do LVC foi identificado pela reaçao ern cadeia da polimerase Nested (PCR Nested), e pelo isolarnento viral. 0 virus foi isolado em 7,1 % das amostras. A PCR identificou 0 DNA pro-viral ern 35,7% do total das amostras: 17,9% nas amostras lavadas e 53,6% das amostras de semen integrais. 0 dano ao testiculo permite maior fluxo do virus para o semen, pois antes do dano, 21,4% das amostras foram positivas e pos-dano, 50%. A transmissao do LVC pelo semen de reprodutores caprinos e potencializada pela presença de inflamaçoes testiculares e pelo fato de o semen criopreservado conter o LVC na forma infectante. [Risk factors in caprine lentivirus transmission through semen]. Abstract: The objective of this work was to evaluate the presence of the DNA provirus of the caprine lentivirus (LVC) in ejaculates of naturally infected males, and to verify the influence of the wash of the semen as well as the presence of testicle inflammation on the viral load. Eight semen collections of seven soropositive reproducers were accomplished, four before testicle injury and four after injury. Amongst the collections carried out at the same week, in one the ejaculate was washed, to withdraw the plasma seminal, and in the other it was not. The provirus DNA was identified both by Nested polymerase chain reaction technique (Nested PCR) and by the viral isolation. The virus was isolated in 7.1% of the samples. The PCR identified the provirus DNA in 35.7% of all samples, 17.9% in the washed samples and 53.6% of the integral semen samples. The injury of the testicle tends to greater flow of virus for the semen, therefore, before injury, 21.4% of the samples were positive and after-injury, 50%. Risk of transmission of the LVC by semen of goat reproducers is strengthened by the presence of testicle inflammations and the fact that the criopreserved semen contains the LVC in infecting form.
650 $aAnimal reproduction
650 $aArtificial insemination
650 $aCaprine arthritis encephalitis virus
650 $aGoats
650 $aInfectious diseases
650 $aLentivirus
650 $aReproductive disorders
650 $aVirus transmission
650 $aCaprino
650 $aReprodução animal
650 $aSêmen
650 $aTransmissão de doença
653 $aArtrite Encefalite
653 $aCAEV
653 $aInfected bucks
700 1 $aGOUVEIA, A. M. G.
700 1 $aMARTINS, A. de S.
700 1 $aPINHEIRO, R. R.
700 1 $aSANTOS, D. O.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 41, n. 8, p. 1313-1319, ago. 2006.
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