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| Registros recuperados : 26 | |
| Registros recuperados : 26 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
06/01/2016 |
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Data da última atualização: |
26/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
BRASILEIRO, A. C. M.; MORGANTE, C. V.; ARAUJO, A. C. G.; LEAL-BERTIOLI, S. C. M.; SILVA, A. K.; MARTINS, A. C. Q.; VINSON, C. C.; SANTOS, C. M. R.; BONFIM, O.; TOGAWA, R. C.; SARAIVA, M. A. P.; BERTIOLI, D. J.; GUIMARAES, P. M. |
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Afiliação: |
ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN; CAROLINA VIANNA MORGANTE, CPATSA; ANA CLAUDIA GUERRA DE ARAUJO, CENARGEN; SORAYA CRISTINA DE M LEAL BERTIOLI, CENARGEN; AMANDA K. SILVA; ANDRESSA C. Q. MARTINS; CHRISTINA C. VINSON; CANDICE M. R. SANTOS, CONAB; ORZENIL BONFIM DA SILVA JUNIOR, CENARGEN; ROBERTO COITI TOGAWA, CENARGEN; MARIO ALFREDO DE PASSOS SARAIVA, CENARGEN; DAVID J. BERTIOLI, UNB; PATRICIA MESSEMBERG GUIMARAES, CENARGEN. |
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Título: |
Transcriptome profiling of wild Arachis from water-limited environments uncovers drought tolerance candidate genes. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plant Molecular Biology Reporter, v. 33, p. 1876-1892, 2015. |
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DOI: |
10.1007/s11105-015-0882-x |
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Idioma: |
Inglês |
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Conteúdo: |
Peanut (Arachis hypogaeaL.) is an important le-gume cultivated mostly in drought-prone areas where its pro-ductivity can be limited by water scarcity. The development of more drought-tolerant varieties is, therefore, a priority for pea-nut breeding programs worldwide. In contrast to cultivated peanut, wild relatives have a broader genetic diversity and constitute a rich source of resistance/tolerance alleles to biotic and abiotic stresses. The present study takes advantage of this diversity to identify drought-responsive genes by analyzing the expression profile of two wild species, Arachis duranensis and Arachis magna (AA and BB genomes, respectively), in response to progressive water deficit in soil. Data analysi from leaves and roots of A. duranensis (454 sequencing) and A. magna (suppression subtractive hybridization (SSH)) stressed and control complementary DNA (cDNA) libraries revealed several differentially expressed genes in silico, and 44 of them were selected for further validation by quantitative RT-PCR (qRT-PCR). This allowed the identification of drought-responsive candidate genes, such as Expansin, Nitrilase, NAC ,and bZIP transcription factors, displaying sig-nificant levels of differential expression during stress imposition in both species. This is the first report on identification of differentially expressed genes under drought stress and recov-ery in wild Arachis species. The generated transcriptome data.besides being a valuable resource for gene discovery, will allow the characterization of new alleles and development of molecular markers associated with drought responses in pea-nut. These together constitute important tools for the peanut breeding program and also contribute to a better comprehen-sion of gene modulation in response to water deficit and rehydration. MenosPeanut (Arachis hypogaeaL.) is an important le-gume cultivated mostly in drought-prone areas where its pro-ductivity can be limited by water scarcity. The development of more drought-tolerant varieties is, therefore, a priority for pea-nut breeding programs worldwide. In contrast to cultivated peanut, wild relatives have a broader genetic diversity and constitute a rich source of resistance/tolerance alleles to biotic and abiotic stresses. The present study takes advantage of this diversity to identify drought-responsive genes by analyzing the expression profile of two wild species, Arachis duranensis and Arachis magna (AA and BB genomes, respectively), in response to progressive water deficit in soil. Data analysi from leaves and roots of A. duranensis (454 sequencing) and A. magna (suppression subtractive hybridization (SSH)) stressed and control complementary DNA (cDNA) libraries revealed several differentially expressed genes in silico, and 44 of them were selected for further validation by quantitative RT-PCR (qRT-PCR). This allowed the identification of drought-responsive candidate genes, such as Expansin, Nitrilase, NAC ,and bZIP transcription factors, displaying sig-nificant levels of differential expression during stress imposition in both species. This is the first report on identification of differentially expressed genes under drought stress and recov-ery in wild Arachis species. The generated transcriptome data.besides being a valuable resource for gene discover... Mostrar Tudo |
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Palavras-Chave: |
4 Sequencing; 454 Sequencing; Cultura resistente a seca; Differential gene expression; Dry-down; Gene tolerante; Peanut wild relatives; QRT-PCR; SSHlibraries. |
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Thesagro: |
Amendoim; Biologia molecular; Resistência a Seca. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/137981/1/Brasileiro-et-al-2015.pdf
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Marc: |
LEADER 03070naa a2200421 a 4500
001 2033034
005 2024-04-26
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1007/s11105-015-0882-x$2DOI
100 1 $aBRASILEIRO, A. C. M.
245 $aTranscriptome profiling of wild Arachis from water-limited environments uncovers drought tolerance candidate genes.$h[electronic resource]
260 $c2015
520 $aPeanut (Arachis hypogaeaL.) is an important le-gume cultivated mostly in drought-prone areas where its pro-ductivity can be limited by water scarcity. The development of more drought-tolerant varieties is, therefore, a priority for pea-nut breeding programs worldwide. In contrast to cultivated peanut, wild relatives have a broader genetic diversity and constitute a rich source of resistance/tolerance alleles to biotic and abiotic stresses. The present study takes advantage of this diversity to identify drought-responsive genes by analyzing the expression profile of two wild species, Arachis duranensis and Arachis magna (AA and BB genomes, respectively), in response to progressive water deficit in soil. Data analysi from leaves and roots of A. duranensis (454 sequencing) and A. magna (suppression subtractive hybridization (SSH)) stressed and control complementary DNA (cDNA) libraries revealed several differentially expressed genes in silico, and 44 of them were selected for further validation by quantitative RT-PCR (qRT-PCR). This allowed the identification of drought-responsive candidate genes, such as Expansin, Nitrilase, NAC ,and bZIP transcription factors, displaying sig-nificant levels of differential expression during stress imposition in both species. This is the first report on identification of differentially expressed genes under drought stress and recov-ery in wild Arachis species. The generated transcriptome data.besides being a valuable resource for gene discovery, will allow the characterization of new alleles and development of molecular markers associated with drought responses in pea-nut. These together constitute important tools for the peanut breeding program and also contribute to a better comprehen-sion of gene modulation in response to water deficit and rehydration.
650 $aAmendoim
650 $aBiologia molecular
650 $aResistência a Seca
653 $a4 Sequencing
653 $a454 Sequencing
653 $aCultura resistente a seca
653 $aDifferential gene expression
653 $aDry-down
653 $aGene tolerante
653 $aPeanut wild relatives
653 $aQRT-PCR
653 $aSSHlibraries
700 1 $aMORGANTE, C. V.
700 1 $aARAUJO, A. C. G.
700 1 $aLEAL-BERTIOLI, S. C. M.
700 1 $aSILVA, A. K.
700 1 $aMARTINS, A. C. Q.
700 1 $aVINSON, C. C.
700 1 $aSANTOS, C. M. R.
700 1 $aBONFIM, O.
700 1 $aTOGAWA, R. C.
700 1 $aSARAIVA, M. A. P.
700 1 $aBERTIOLI, D. J.
700 1 $aGUIMARAES, P. M.
773 $tPlant Molecular Biology Reporter$gv. 33, p. 1876-1892, 2015.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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