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Registro Completo |
Biblioteca(s): |
Embrapa Agropecuária Oeste; Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental; Embrapa Pantanal; Embrapa Unidades Centrais. |
Data corrente: |
04/06/1997 |
Data da última atualização: |
26/04/2018 |
Autoria: |
STEIN, R. L. B.; ITO, T.; ALBUQUERQUE, F. C. de; NASCIMENTO R. M. do. |
Afiliação: |
RUTH LINDA BENCHIMOL, CPATU; TSUTAE ITO, CONSULTOR CPATU/JICA; FERNANDO CARNEIRO DE ALBUQUERQUE, CPATU; RISALDO MUNIZ DO NASCIMENTO, BOLSISTA CPATU. |
Título: |
Produção artificial de basidiocarpos de Crinipellis perniciosa do cupuaçuzeiro em meio de farelo-vermiculita. |
Ano de publicação: |
1996 |
Fonte/Imprenta: |
Belém, PA: EMBRAPA-CPATU, 1996. |
Páginas: |
15 p. |
Série: |
(EMBRAPA-CPATU. Boletim de pesquisa, 167). |
Idioma: |
Português |
Conteúdo: |
A produção artificial de basidiocarpos pelo isolado C990 de Crinipellis perniciosa do cupuaçuzeiro foi obtida após a colonização, durante oito semanas, pela fase saprótica do patógeno em meio de cultura artificial à base de farelo-vermiculita. |
Palavras-Chave: |
Basidiocarpos; Brasil; Capuacu; Cultura média; Disease; Fungus; Plant; Theobroma agrandiflorium. |
Thesagro: |
Crinipellis perniciosa; Cupuaçu; Doença; Fruta; Fungo; Meio de cultura; Planta; Theobroma Grandiflorum. |
Thesaurus Nal: |
Amazonia; Culture media; Fruits; Fungi. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/42901/1/Boletim-Pesquisa-167-CPATU.pdf
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Marc: |
LEADER 01323nam a2200409 a 4500 001 1374452 005 2018-04-26 008 1996 bl uuuu u0uu1 u #d 100 1 $aSTEIN, R. L. B. 245 $aProdução artificial de basidiocarpos de Crinipellis perniciosa do cupuaçuzeiro em meio de farelo-vermiculita. 260 $aBelém, PA: EMBRAPA-CPATU$c1996 300 $a15 p. 490 $a(EMBRAPA-CPATU. Boletim de pesquisa, 167). 520 $aA produção artificial de basidiocarpos pelo isolado C990 de Crinipellis perniciosa do cupuaçuzeiro foi obtida após a colonização, durante oito semanas, pela fase saprótica do patógeno em meio de cultura artificial à base de farelo-vermiculita. 650 $aAmazonia 650 $aCulture media 650 $aFruits 650 $aFungi 650 $aCrinipellis perniciosa 650 $aCupuaçu 650 $aDoença 650 $aFruta 650 $aFungo 650 $aMeio de cultura 650 $aPlanta 650 $aTheobroma Grandiflorum 653 $aBasidiocarpos 653 $aBrasil 653 $aCapuacu 653 $aCultura média 653 $aDisease 653 $aFungus 653 $aPlant 653 $aTheobroma agrandiflorium 700 1 $aITO, T. 700 1 $aALBUQUERQUE, F. C. de 700 1 $aNASCIMENTO R. M. do
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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Registro Completo
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
24/10/2017 |
Data da última atualização: |
01/02/2021 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
CLEMENTE, Z.; SILVA, G. H. da; MARTINEZ, D. S. T.; MAURER-MORELLI, C. V.; NUNES, M. C. S.; THOMAZ, A. A.; CASTRO, V. L. S. S. de. |
Afiliação: |
VERA LUCIA SCHERHOLZ S DE CASTRO, CNPMA. |
Título: |
Exploring the mechanisms of graphene oxide toxicity to zebrafish embryo. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Applied Research in Toxicology, v. 2, p. 56, 2017. Suppl. 1. Abstracts of International Symposium on Toxicity Assesment, 18., 2017, Limeira. Ref. ISTA18-705454. |
Idioma: |
Inglês |
Conteúdo: |
Many applications of graphene oxide (GO) are still in the research phase, but its promising uses have raised concerns about their risks to human health and the environment. Environmental factors, as the presence of humic substances in aquatic environment, can affect nanomaterials behavior and its toxicological effects. A previous study of our group revealed that the exposure of zebrafish embryos to GO is related to a reduction in total body length and in acetylcholinesterase activity. Thus, the aim of this study was to investigate the implications of GO exposure in muscular development and acetylcholinesterase expression. Zebrafish embryos (n=48/group) were exposed to GO (100 mg/L) in the presence or absence of humic acid (HA, 20 mg/L) during 5 days. Larvae were not feed during the experiment and were exposed under a 14/10 h light/dark cycle, 26 ± 1oC. The mortality in all groups was below 10% and no malformation was observed. At the end of the experiment, alive larvae were fixed with PFA 4% to evaluate its muscular morphology (confocal microscopy, SHG technique) or frozen in liquid nitrogen to perform RT-PCR analysis (AchE expression). Measurements of sarcomere and somite length were performed. No change in muscular tissue was observed, neither differences in AchE expression among groups were detected. So, further experiments have to be performed to clarify the mechanisms of graphene toxicity. FAPESP grants 2014/01995-9, 2014/12891-0, 2014/15640-8. |
Palavras-Chave: |
Zebrafish. |
Categoria do assunto: |
W Química e Física |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/165499/1/RA-CastroVLSS-ISTA18-ART-2017-PA....pdf
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Marc: |
LEADER 02169nam a2200193 a 4500 001 2078089 005 2021-02-01 008 2017 bl uuuu u00u1 u #d 100 1 $aCLEMENTE, Z. 245 $aExploring the mechanisms of graphene oxide toxicity to zebrafish embryo.$h[electronic resource] 260 $aApplied Research in Toxicology, v. 2, p. 56, 2017. Suppl. 1. Abstracts of International Symposium on Toxicity Assesment, 18., 2017, Limeira. Ref. ISTA18-705454.$c2017 520 $aMany applications of graphene oxide (GO) are still in the research phase, but its promising uses have raised concerns about their risks to human health and the environment. Environmental factors, as the presence of humic substances in aquatic environment, can affect nanomaterials behavior and its toxicological effects. A previous study of our group revealed that the exposure of zebrafish embryos to GO is related to a reduction in total body length and in acetylcholinesterase activity. Thus, the aim of this study was to investigate the implications of GO exposure in muscular development and acetylcholinesterase expression. Zebrafish embryos (n=48/group) were exposed to GO (100 mg/L) in the presence or absence of humic acid (HA, 20 mg/L) during 5 days. Larvae were not feed during the experiment and were exposed under a 14/10 h light/dark cycle, 26 ± 1oC. The mortality in all groups was below 10% and no malformation was observed. At the end of the experiment, alive larvae were fixed with PFA 4% to evaluate its muscular morphology (confocal microscopy, SHG technique) or frozen in liquid nitrogen to perform RT-PCR analysis (AchE expression). Measurements of sarcomere and somite length were performed. No change in muscular tissue was observed, neither differences in AchE expression among groups were detected. So, further experiments have to be performed to clarify the mechanisms of graphene toxicity. FAPESP grants 2014/01995-9, 2014/12891-0, 2014/15640-8. 653 $aZebrafish 700 1 $aSILVA, G. H. da 700 1 $aMARTINEZ, D. S. T. 700 1 $aMAURER-MORELLI, C. V. 700 1 $aNUNES, M. C. S. 700 1 $aTHOMAZ, A. A. 700 1 $aCASTRO, V. L. S. S. de
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Embrapa Meio Ambiente (CNPMA) |
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