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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
30/08/2002 |
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Data da última atualização: |
26/01/2024 |
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Autoria: |
MAUDET, C.; TABERLET, P. |
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Título: |
Detection of cows' milk in goats' cheeses inferred from mitochondrial DNA polymorphism. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Journal of Dairey Research, v. 68, n. 2, p. 229-235, May, 2001. |
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DOI: |
https://doi.org/10.1017/S0022029901004794 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: A new polymerase chain reaction (PCR)-based method was developed to detect cows' milk in goat cheese. This method is based on mitochondrial DNA (mtDNA) control region sequence variations. DNA extractions from 150 mg of cheese were carried out using a spin column-based method. Subsequent PCR amplifications of DNA were performed with cow specific primers, demonstrating the ability to detect cows' milk in a variety of cheeses. This simple approach provides high quality DNA, and is shown to be very sensitive, with a detection limit of less than 0·1% of cows' milk. Analysis of an agarose gel digital image allows a rough estimation of the percentage of cows' milk used in adulteration. |
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Palavras-Chave: |
Cow milk; Detecção; Deteccao em PCR; DNA Mitochondrial; DNA mitocondrial; Genética mitocondrial; Leite de Vaca; PCR. |
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Thesagro: |
Bovino; Caprino; Citogenética Animal; DNA; Leite de Cabra; Polimorfismo; Queijo. |
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Thesaurus Nal: |
Adulterated products; Cattle; Cheese milk; Cheeses; Detection; Food analysis; Food chemistry; Food contamination; Genetic polymorphism; Goat cheese; Goat milk; Goats; Milk proteins; Mitochondrial DNA; Polymerase chain reaction. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02064naa a2200505 a 4500
001 1526433
005 2024-01-26
008 2001 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1017/S0022029901004794$2DOI
100 1 $aMAUDET, C.
245 $aDetection of cows' milk in goats' cheeses inferred from mitochondrial DNA polymorphism.$h[electronic resource]
260 $c2001
520 $aAbstract: A new polymerase chain reaction (PCR)-based method was developed to detect cows' milk in goat cheese. This method is based on mitochondrial DNA (mtDNA) control region sequence variations. DNA extractions from 150 mg of cheese were carried out using a spin column-based method. Subsequent PCR amplifications of DNA were performed with cow specific primers, demonstrating the ability to detect cows' milk in a variety of cheeses. This simple approach provides high quality DNA, and is shown to be very sensitive, with a detection limit of less than 0·1% of cows' milk. Analysis of an agarose gel digital image allows a rough estimation of the percentage of cows' milk used in adulteration.
650 $aAdulterated products
650 $aCattle
650 $aCheese milk
650 $aCheeses
650 $aDetection
650 $aFood analysis
650 $aFood chemistry
650 $aFood contamination
650 $aGenetic polymorphism
650 $aGoat cheese
650 $aGoat milk
650 $aGoats
650 $aMilk proteins
650 $aMitochondrial DNA
650 $aPolymerase chain reaction
650 $aBovino
650 $aCaprino
650 $aCitogenética Animal
650 $aDNA
650 $aLeite de Cabra
650 $aPolimorfismo
650 $aQueijo
653 $aCow milk
653 $aDetecção
653 $aDeteccao em PCR
653 $aDNA Mitochondrial
653 $aDNA mitocondrial
653 $aGenética mitocondrial
653 $aLeite de Vaca
653 $aPCR
700 1 $aTABERLET, P.
773 $tJournal of Dairey Research$gv. 68, n. 2, p. 229-235, May, 2001.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
03/01/2006 |
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Data da última atualização: |
03/01/2006 |
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Autoria: |
MARTIN-NETO, L.; NASCIMENTO, O. R.; TABAK, M.; CARACELLI, I. |
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Título: |
The mechanism of reaction of nitrosyl with met- and oxymyoglobin: an ESR study. |
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Ano de publicação: |
1988 |
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Fonte/Imprenta: |
Biochimica et Biophysica Acta, Amsterdam, v.956, p. 189-196, 1988. |
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Idioma: |
Inglês |
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Conteúdo: |
In this ESR work we have studied the pentacoordinate symmetry in horse, whale and sperm-whale myoglobin (Mb) in different physical states such as solution and powder. Experiments were performed in which the following parameters were varied: the sample temperature, pH, reactiohn time with NO, and NO concentration. The results enabled us to explain the NO reaction mechanism in the oxy and met forms of myoglobin. The study of powder samples at different degrees of hydration allowed us to identify the diamagnetic intermediate species existent in the reaction of NO with met-Mb proposed in the literature. The results presented explain adequately the pH effect and temperature dependence observed in the ESR spectra obtained using the met-Mb sample solutions form Sigma Chemical Co., which consist of a mixture (13%) of Mb-O2. |
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Palavras-Chave: |
ESR; Mb; Nitrosyl myoglobin; Reaction mechanism. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01397naa a2200205 a 4500
001 1029171
005 2006-01-03
008 1988 bl --- 0-- u #d
100 1 $aMARTIN-NETO, L.
245 $aThe mechanism of reaction of nitrosyl with met- and oxymyoglobin$ban ESR study.
260 $c1988
520 $aIn this ESR work we have studied the pentacoordinate symmetry in horse, whale and sperm-whale myoglobin (Mb) in different physical states such as solution and powder. Experiments were performed in which the following parameters were varied: the sample temperature, pH, reactiohn time with NO, and NO concentration. The results enabled us to explain the NO reaction mechanism in the oxy and met forms of myoglobin. The study of powder samples at different degrees of hydration allowed us to identify the diamagnetic intermediate species existent in the reaction of NO with met-Mb proposed in the literature. The results presented explain adequately the pH effect and temperature dependence observed in the ESR spectra obtained using the met-Mb sample solutions form Sigma Chemical Co., which consist of a mixture (13%) of Mb-O2.
653 $aESR
653 $aMb
653 $aNitrosyl myoglobin
653 $aReaction mechanism
700 1 $aNASCIMENTO, O. R.
700 1 $aTABAK, M.
700 1 $aCARACELLI, I.
773 $tBiochimica et Biophysica Acta, Amsterdam$gv.956, p. 189-196, 1988.
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